1. Serum interleukin-1 receptor antagonist is an early indicator of colitis onset in Galphai2-deficient mice.
- Author
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Hultgren OH, Berglund M, Bjursten M, and Hultgren Hörnquist E
- Subjects
- Animals, Biomarkers, Colitis diagnosis, GTP-Binding Protein alpha Subunit, Gi2 genetics, GTP-Binding Protein alpha Subunit, Gi2 physiology, Inflammatory Bowel Diseases diagnosis, Interleukin 1 Receptor Antagonist Protein, Male, Mice, Mice, Inbred C57BL, Organ Size, Spleen pathology, Colitis blood, GTP-Binding Protein alpha Subunit, Gi2 deficiency, Interleukin-18 blood, Sialoglycoproteins blood
- Abstract
Aim: To study the serum concentration of IL-1beta, IL-1 receptor antagonist (IL-1Ra) and IL-18 in Galphai2-deficient mice at the age of 6 (healthy), 12 (pre-colitic) and 24 wk (colitic) and in healthy control mice., Methods: At the time of killing, serum samples were collected and IL-1beta, IL-1Ra and IL-18 levels were measured using enzyme-linked immunosorbent assays., Results: Serum concentration of IL-1Ra was significantly increased in pre-colitic (median: 524 ng/L; P=0.02) and colitic (450 ng/L; P=0.01), but not in healthy (196 ng/L) Galphai2-deficient mice as compared with controls (217 ng/L). Serum concentrations of IL-1beta did not differ between Galphai2-deficient mice and their controls, irrespective of age, IL-18 was significantly increased in colitic, but not in pre-colitic mice compared with controls (510 ng/L vs 190 ng/L; P=0.05)., Conclusion: The increased serum concentrations of IL-18 and IL-1Ra in established diseases are suggested as markers of ongoing colitis. Interestingly, the significantly increased serum concentration of IL-1Ra in pre-colitic mice is found to be an early marker of disease progression.
- Published
- 2006
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