1. In vivo and in vitro effect of the Haemophilus influenzae lipopolysaccharide on ciliated respiratory epithelium.
- Author
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Raichvarg D, Siou G, Dubreuil A, Bonnaire Y, Brossard C, and Boudène C
- Subjects
- Animals, Epithelium physiology, Movement drug effects, Rabbits, Rats, Trachea drug effects, Cilia physiology, Haemophilus influenzae analysis, Lipopolysaccharides pharmacology, Trachea physiology
- Abstract
The lipopolysaccharide of Haemophilus influenzae is presumed to have a toxic effect on the tracheal epithelium, and then induce a bronchial obstruction. This activity of LPS was studied in vitro on the ciliary beat using a photo-oscillographic apparatus, and in vivo on the rabbit trachea. Neither modification of ciliary beat frequency, nor epithelial damage in the rabbit trachea was observed after a single administration of LPS. In contrast, histopathologic changes were observed in vivo when the intratracheal administration of H. influenzae LPS was followed 24 h later by an intravenous injection of the same LPS. These experimental models seem thus to implicate a Shwartzman type cellular necrosis in the trachea in vivo in the absence of a direct toxic effect of endotoxin itself on trachea in vivo or in vitro.
- Published
- 1982