1. Neutrophil-mediated mechanisms in non-vascular Behçet’s syndrome
- Author
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Alessandra Bettiol, MATTEO BECATTI, Elena Slvestri, Flavia Rita Argento, Eleonora Fini, Amanda Mannucci, Silvia Galora, Irene Mattioli, Maria Letizia Urban, Danilo Malandrino, Adalgisa Palermo, Niccolò Taddei, Giacomo Emmi, Domenico Prisco, and Claudia Fiorillo
- Abstract
Objective: Behçet’s syndrome (BS) is a systemic vasculitis with several clinical manifestations. Neutrophils hyperactivation mediates vascular BS involvement, via a massive production of reactive oxygen species (ROS) and the release of neutrophil extracellular traps (NETs). We investigated neutrophil-mediated mechanisms of damage in non-vascular BS manifestations and explored in vitro the effects of colchicine in counteracting these mechanisms. Methods: NETs and intracellular ROS production was assessed in blood samples from 80 BS patients (46 with active non-vascular BS, 34 with inactive disease) and 80 healthy controls. Moreover, isolated neutrophils were incubated for 1 hour with an oxidating agent (2,2′-azobis (2-amidinopropane) dihydrochloride; 250nM), and the ability of pure colchicine pre-treatment (100ng/ml) to counteract oxidation-induced damage was assessed. Results: Patients with active non-vascular BS had remarkably increased NET levels [21.2 (IQR 18.3-25.9) mU/ml] compared to patients with inactive disease [16.8 (13.3-20.2) mU/ml] and to controls [7.1 (5.1-8.7) mU/ml], p
- Published
- 2021