1. 3D printing of maize protein by fused deposition modeling
- Author
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Didier Marion, Laurent Chaunier, Bénédicte Bakan, Eric Leroy, Bernard Madec, Denis Lourdin, Guy Della Valle, Michèle Dalgalarrondo, Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de la Recherche Agronomique (INRA), Laboratoire de génie des procédés - environnement - agroalimentaire (GEPEA), Institut Universitaire de Technologie - Nantes (IUT Nantes), Université de Nantes (UN)-Université de Nantes (UN)-Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Institut Universitaire de Technologie Saint-Nazaire (IUT Saint-Nazaire), Université de Nantes (UN)-Ecole Polytechnique de l'Université de Nantes (EPUN), Université de Nantes (UN)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Centre National de la Recherche Scientifique (CNRS)-Université Bretagne Loire (UBL)-IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Institut Universitaire de Technologie - La Roche-sur-Yon (IUT La Roche-sur-Yon), Université de Nantes (UN), Matrices Aliments Procédés Propriétés Structure - Sensoriel (GEPEA-MAPS2), Université de Nantes (UN)-Institut Universitaire de Technologie - Nantes (IUT Nantes), École Centrale de Nantes (ECN), Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Université Bretagne Loire (UBL)-IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Université Bretagne Loire (UBL)-IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Centre National de la Recherche Scientifique (CNRS)-Ecole Polytechnique de l'Université de Nantes (EPUN), Université de Nantes (UN)-Université de Nantes (UN)-Institut Universitaire de Technologie - Nantes (IUT Nantes), Université de Nantes (UN)-Institut Universitaire de Technologie Saint-Nazaire (IUT Saint-Nazaire), Université de Nantes (UN)-Institut Universitaire de Technologie - La Roche-sur-Yon (IUT La Roche-sur-Yon), Université de Nantes (UN)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Université Bretagne Loire (UBL), Polymer Processing Society. Akron, Ohio, USA., and Chaunier, Laurent
- Subjects
Biopolymer ,FDM ,Materials science ,Biocompatibility ,Polymers ,Additive Manufacturing ,Zein ,02 engineering and technology ,3D Printing ,Corn ,Protein ,Endosperm ,law.invention ,chemistry.chemical_compound ,0404 agricultural biotechnology ,law ,Glycerol ,[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering ,Relative humidity ,Elastic modulus ,ComputingMilieux_MISCELLANEOUS ,chemistry.chemical_classification ,Fused deposition modeling ,04 agricultural and veterinary sciences ,Polymer ,021001 nanoscience & nanotechnology ,040401 food science ,Polymères ,[CHIM.POLY]Chemical Sciences/Polymers ,chemistry ,Chemical engineering ,0210 nano-technology ,Glass transition - Abstract
National audience; Additive Manufacturing (AM) opens new perspectives for biopolymers to obtain functional parts, like biomedical devices, by exploiting their biocompatibility and resorbability. Plasticized materials from zein, a storage protein from maize seed endosperm, display thermomechanical properties that could match with Fused Deposition Modeling (FDM). The objective of this work was to evaluate their thenno-rheological behavior and their structural modifications during processing. 20% glycerol was added to conunercial zein, containing naturally about 4% lipids and 5% water. After storage at intermediate relative humidity (RH=59%). its glass transition temperature, measured by DSC, was T(g)=42°C. The main mechanical relaxation, measured by DMA, was found at Tα=50°C, leading to a drop of the elastic modulus from E'=1.1GPa, at ambient temperature, to E'=0.6MPa at Tα+100°C. These values are in a similar range as those of standard polymers used for AM-FDM processing, such as PLA and ABS. The structure of zein was characterized at different scales by SDS-PAGE, reversed-phase HPLC, FTIR and WAXS, at each processing stage: (i) the initial formulation of the plasticized powdery material, (ii) after extrusion at 130°C for shaping printable filaments, and (iii) after deposition through the 3D printer nozzle (Ø(nozzle)=0.5mm, T(printing)=130°C, v(printing)≈10mm/s). The presence of disulfide bond cross-links was evidenced in extruded filaments and the level after printing. WAXS showed that, in these conditions, no molecular orientation was obtained in the deposited material. By tuning zein-based printable compositions, these results open the field of their processing as resorbable printed parts, with a controlled geometry and a designed tridimensional structure.
- Published
- 2017