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Healthy drinking water is a source of life and comes from many sources, most notably groundwater which is infectious in both chemical and biological forms. One of the biological factors is parasitic infections, especially protozoa, causing deaths or erosion of the physical and mental power of infected individuals annually. The purpose of this study is to investigate the prevalence of parasites in drinking water wells in the city of Rumeshgan. Cross-sectional study is designed and 132 samples of 2.5 liters of drinking water from wells in the city of Rumeshgan are collected haphazardly. The parasites in each of the samples are concentrated on the surface of the filter paper using a suction pump, then, by washing the filter paper and centrifuging the solution of the rinse, the condensed parasites in the sediment are stained with Logel and Zill-Nelson methods, using an optical microscope the survey was conducted. The parasites in each of the samples are concentrated on the surface of the filter paper using a suction pump. Then, by washing the filter paper and centrifuging the solution of the washer, the condensed parasites are stained in the sediments by Logel and Zill-Nelson methods and surveyed by optical microscope. Data analysis is performed using descriptive and analytical statistics. In current study, 14.4% of drinking water wells are infected with protozoa and pathogenic parasites and the most common parasitic infection is cryptosporidium that has appropriated of 9.8% of water wells. Parasitic contamination with the well water depth of [OR = 5. 2 95% CI (2-6.8)] and the distance of water well to the sewage well [OR = 3. 5 95% CI (1-3.5)] had a significant relationship. SIG <0. 05 Interpretation: Contamination of 14.4% of drinking water from wells to pathogens causes at least annual referring of Consumer population to the physician and to the imposition of economic and health damage especially in hot seasons. Statistical results, along with other reasons, indicate that drinking water pollution is due to leakage of sewage sludge, which is suggested to provide water for plumbing or controlling for people. Otherwise, the epidemic of water borne diseases is not unexpected. [ABSTRACT FROM AUTHOR]