1. Caracterización molecular y bioinformática de la proteína CagA de Helicobacter pylori a partir de biopsias gástricas de pacientes colombianos.
- Author
-
Amador, Paula Nicole Acosta, Delgado, María del Pilar, Ortiz, María Camila Montealegre, de Polanco, María Magdalena Echeverry, and Henao, Carlos Jaramillo
- Subjects
- *
BIOINFORMATICS , *MOLECULAR biology , *HELICOBACTER pylori , *DIETARY proteins , *GASTRIC diseases , *BIOPSY , *PHOSPHORYLATION , *TYROSINE - Abstract
Helicobacter pylori CagA protein, the cagA gen product, has been considered as a virulence factor associated with a considerable increase risk for develops severe gastric illness. The purpose of this research was to design a molecular and bioinformatics strategy that allowed the establishment of phosphorylation status of the tyrosine residue of the CagA protein. The amplification and sequencing of the variable fragment region of cagA in the positive CagA samples were used to do the bioinformatics analysis in order to establish the characteristics of the EPIYA motifs. The presence of the EPIYA-A and EPIYA-B motifs, followed by one or two EPIYA-C repetitions, similar to those reported previously for occidental countries were set up. From the different bioinformatics applications that were employed only one group of tools proved to be useful to characterize the repeated units presents in the CagA protein. [ABSTRACT FROM AUTHOR]
- Published
- 2009