1. Silencing of Lysyl Oxidase Gene Expression by RNA Interference Suppresses Metastasis of Breast Cancer
- Author
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Wei Wei, Wei Tang, Jianlun Liu, Jing-tao Li, Xiao Zhou, Yi Jiang, and Hua-Wei Yang
- Subjects
Cancer Research ,Epidemiology ,Breast Neoplasms ,Lysyl oxidase ,Biology ,Real-Time Polymerase Chain Reaction ,Transfection ,Metastasis ,Protein-Lysine 6-Oxidase ,Breast cancer ,Cell Movement ,RNA interference ,Cell Line, Tumor ,medicine ,Humans ,Gene silencing ,Neoplasm Invasiveness ,Gene Silencing ,RNA, Messenger ,skin and connective tissue diseases ,Public Health, Environmental and Occupational Health ,Cell migration ,Middle Aged ,medicine.disease ,Molecular biology ,Real-time polymerase chain reaction ,Matrix Metalloproteinase 9 ,Oncology ,Lymphatic Metastasis ,Matrix Metalloproteinase 2 ,Immunohistochemistry ,Female ,RNA Interference - Abstract
Objective: The aim of this study was to investigate possible mechanisms of LOX gene effects on invasion and metastasis of breast cancer cells by RNA interference. Methods: LOX-RNAi-LV was designed, synthesized, and then transfected into a breast cancer cell line (MDA-MB-231). Expression of LOX, MMP-2 and MMP-9 was determined by real-time PCR, and protein expression of LOX by Western blotting. Cell migration and invasiveness were assessed with Transwell chambers. A total of 111 cases of breast cancer tissues, cancer-adjacent normal breast tissues, and 20 cases of benign lesion tissues were assessed by immunohistochemistry. Results: Expression of LOX mRNA and protein was suppressed, and the expression of MMP-2 and MMP-9 was significantly lower in the RNAi group than the control group (P
- Published
- 2012
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