1. Towards Better Insect Management Strategy: Restriction Of Insecticidal Gene Expression To Biting Sites In Transgenic Cotton
- Author
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Allah Bakhsh, Surendra Barpete, Emine Anayol, Omer Karakoc, Deniz Köm, Khalid Mahmood Khawar, Cengiz Sancak, Burak Önol, Selma Onarıcı, S. Özcan, Levent Ünlü, Saber Delpasand Khabbazi, Sebahattin Özcan, Muhammad Aasim, 0-Belirlenecek, Aasim, Muhammad, khawar, khalid mahmood -- 0000-0001-5110-6014, Bakhsh, Allah -- 0000-0003-3561-7863, Sancak, Cengiz -- 0000-0002-3091-2639, Aasim, Muhammad -- 0000-0002-8524-9029, Khabbazi, Saber Delpasand -- 0000-0002-1697-9327, Barpete, Surendra -- 0000-0002-5547-8488, and [Anayol, Emine -- Ozcan, Sancar Fatih] Minist Food Agr & Livestock, Cent Res Inst Field Crops, Ankara, Turkey -- [Bakhsh, Allah] Nigde Univ, Dept Agr Genet Engn, Nigde, Turkey -- [Karakoc, Omer Cem] Univ Cankiri Karatekin, Yaprakli Vocat Sch, Cankiri, Turkey -- [Onarici, Selma] Sci & Tech Council Turkey, Genet Engn & Biotechnol Inst, Gebze, Turkey -- [Kom, Deniz -- Barpete, Surendra -- Khabbazi, Saber D. -- Onol, Burak -- Sancak, Cengiz -- Khawar, Khalid M. -- Ozcan, Sebahattin] Ankara Univ, Fac Agr, Dept Field Crops, TR-06100 Ankara, Turkey -- [Aasim, Muhammad] Univ Karamanoglu Mehmet Bey, Dept Biol, Karaman, Turkey -- [Unlu, Levent] Selcuk Univ, Dept Plant Protect, Konya, Turkey
- Subjects
Confined expression ,0106 biological sciences ,0301 basic medicine ,Genetics ,biology ,Period (gene) ,Transgene ,fungi ,Insect management ,food and beverages ,Plant Science ,Genetically modified crops ,Agrobacterium tumefaciens ,Spodoptera ,biology.organism_classification ,01 natural sciences ,03 medical and health sciences ,030104 developmental biology ,Cry1Ac ,Genetic modification ,Gene expression ,Gene ,Insect resistance ,010606 plant biology & botany ,Biotechnology - Abstract
WOS: 000373886900005, Most of the commercialized Bt crops express cry genes under 35S promoter that induces strong gene expression in all plant parts. However, targeted foreign gene expression in plants is esteemed more important as public may be likely to accept 'less intrusive' expression of transgene. We developed plant expression constructs harboring cry1Ac gene under control of wound-inducible promoter (AoPR1) to confine Bt gene expression in insect wounding parts of the plants in comparison with cry1Ac gene under the control of 35S promoter. The constructs were used to transform four Turkish cotton cultivars (GSN-12, STN-468, Ozbek-100 and Ayhan-107) through Agrobacterium tumefaciens strains GV2260 containing binary vectors p35SAcBAR.101 and AoPR1AcBAR.101 harboring cry1Ac gene under control of 35S and AoPR1, respectively. Phosphinothricin (PPT) was used at concentration of 5 mg L-1 for selection of primary transformants. The primary transformants were analyzed for transgene presence and expression standard molecular techniques. The transformants exhibited appreciable mortality rates against larvae of Spodoptera exigua and S. littoralis. It was found that mechanical wounding of T (1) transgenic plants was effective in inducing expression of cry1Ac protein as accumulated levels of cry1Ac protein increased during post-wounding period. We conclude that use of wound-inducible promoter to drive insecticidal gene(s) can be regarded as a valuable insect-resistant management strategy since the promoter activity is limited to insect biting sites of plant. There is no Bt toxin accumulation in unwounded plant organs, seed and crop residues, cotton products and by-products, thus minimizing food and environmental concerns., Scientific and Technological Research Council of Turkey TUBITAK [111O254], The work on development of transgenic cotton in our laboratory is being supported by grants from Scientific and Technological Research Council of Turkey TUBITAK (Project No. 111O254). The authors acknowledge contribution and support of TUBITAK. The authors are also thankful to Leicester University (UK) for giving permission to use AoPR1 promoter for research purposes.
- Published
- 2016