1. Insights From Simulations Into The Mechanism Of Human Topoisomerase I: Explanation For A Seeming Controversy In Experiments
- Author
-
Levent Sari, Neslihan Ucuncuoglu, and Ioan Andricioaei
- Subjects
Models, Molecular ,Protein Conformation ,Stereochemistry ,Mutant ,Hinge ,Quantitative Structure-Activity Relationship ,Molecular Dynamics Simulation ,Biology ,chemistry.chemical_compound ,Molecular dynamics ,Materials Chemistry ,Humans ,Physical and Theoretical Chemistry ,Spectroscopy ,chemistry.chemical_classification ,Topoisomerase ,Cellular Regulation ,DNA ,Computer Graphics and Computer-Aided Design ,Molecular Docking Simulation ,Enzyme ,DNA Topoisomerases, Type I ,chemistry ,biology.protein ,Biophysics ,Nucleic Acid Conformation ,DNA supercoil ,Protein Binding - Abstract
Human topoisomerase-I is a vital enzyme involved in cellular regulation of DNA supercoiling. We extend our previous work on wild type enzyme [13] to study how different enzyme mutants with various parts of the protein clamped by disulfide mutations affect DNA rotation. Three different mutants have been simulated; they are clamped enzyme-DNA systems in which the disulfide bridge is formed by replacing His367 and Ala499, Gly365 and Ser534, and, respectively, Leu429 and Lys436 with Cys pairs. The first of these mutants, a 'distally clamped' enzyme, mimics the experimental study of Carey et al. [11], which reports DNA rotation within the clamped enzyme. The second one, a 'proximal clamp', mimics the study of Woo et al. [12], who do not observe DNA rotation. The third is a newly suggested mutant that clamps the hinge for protein opening; we use it to test a hypothesis on negative supercoil relaxation. Our simulations show that the helical domain alpha 5 totally melts in relaxation of positive supercoils when the enzyme is proximally clamped, while it preserves its structure very well within the distally clamped one. Moreover, a distally clamped protein permits DNA rotations in both directions, while the proximal clamp allows rotations only for negatively supercoiled DNA. These observations reconcile the two seemingly contradictory experimental findings, suggesting that subtle changes in the location of the disulfide bridge alter the mechanism significantly. (C) 2013 Elsevier Inc. All rights reserved.
- Published
- 2013