1. The Role Of Cell Cycle Progression For The Apoptosis Of Cancer Cells Induced By Palladium(Ii)-Saccharinate Complexes Of Terpyridine
- Author
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Engin Ulukaya, Nazli Arda, Omer Kacar, Veysel T. Yilmaz, Ceyda Acilan, Ibrahim Hatipoglu, Buse Cevatemre, Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Biyokimya Anabilim Dalı., Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü., Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü., Cevatemre, Buse, Ulukaya, Engin, Yılmaz, Veysel Turan, AHD-2050-2022, K-5792-2018, and L-7238-2018
- Subjects
0301 basic medicine ,Unclassified drug ,Biochemistry & molecular biology ,Cytotoxicity ,Pyridine ,Clinical Biochemistry ,Ines ,Pharmaceutical Science ,Apoptosis ,Saccharinate complex ,Biochemistry ,Palladium(II) complex ,Cell cycle specific ,HeLa ,In-vitro ,Cancer cell line ,chemistry.chemical_compound ,0302 clinical medicine ,HCT 116 cell line ,Coordination Complexes ,Chemotherapeutic-agents ,Drug Discovery ,Mimosine ,Chemistry, organic ,Cancer ,Fluorescence microscopy ,biology ,Chemistry ,Nocodazole ,Anticancer therapy ,Cell Cycle ,MDA-MB-231 cell line ,HeLa cell line ,Cell cycle ,Metal based anticancer agents ,Cell biology ,Antineoplastic agent ,Drug screening ,030220 oncology & carcinogenesis ,Combination ,Palladium complex ,Molecular Medicine ,Chemistry, medicinal ,Coordination compound ,Human ,Aphidicolin ,inorganic chemicals ,Antineoplastic Agents ,Thymidine Kinase 1 ,Tumor Biomarkers ,G1 phase cell cycle checkpoint ,Article ,Saccharinate(2,2'-6',2'-terpyridine)palladium(II) ,Cell cycle arrest ,03 medical and health sciences ,Cell cycle progression ,Cell Line, Tumor ,Humans ,MTT assay ,Terpyridine ,Molecular Biology ,Mitosis ,Drug effects ,Cytotoxic activity ,Pharmacology & pharmacy ,Protein ,Organic Chemistry ,Demecolcine ,Tumor cell line ,biology.organism_classification ,030104 developmental biology ,Microscopy, Fluorescence ,Cancer cell ,Mitosis inhibitioc ,Cisplatin ,Drug Screening Assays, Antitumor ,Controlled study ,Thymidine - Abstract
Objectives: Palladium complexes are potent and less toxic molecules in comparison to other metal based agents. Here, we characterized two palladium(II) saccharinate complexes with terpyridine for their cell cycle specificity. Materials and methods: Cells were arrested at Gl, Gl/S boundary or mitosis using mimosine, double-Thymidine block, aphidicolin, nocodazole or colcemid, and evaluated based on morphology and flow cytometry. Synchronized cells were treated with the Pd(II) complexes, and viability was measured via MTT assay. Results: While treatment of arrested cells with the Pd(II) complexes resulted in no significant change in cell death in HCT-116 and MDA-MB-231 cells, HeLa cells were more sensitive in S/G1. The main form of cell death was found to be apoptosis. Conclusions: Pd(II) complexes appear to be cell-cycle non-specific, while cell line dependent differences may be observed. Cells die through apoptosis regardless of the cell cycle stage, which makes these complexes more promising as anti-cancer agents. İstanbul Üniversitesi - 36696
- Published
- 2017