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Copyright of Journal of Faculty of Pharmacy of Ankara University / Ankara Üniversitesi Eczacilik Fakültesi Dergisi is the property of Ankara University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Pulmonary veno-occlusive disease (PVOD) is an uncommon disease in humans and animals caused by pulmonary hypertension (PH). The aim of this study was to investigate pathomorphological findings of PVOD in a cat. A mass was detected in the right atrium with echocardiography in an 11-year-old male cat. Following treatments, necropsy of the cat was performed after death due to respiratory failure. Macroscopically, masses were detected in mesenteric lymph node, mediastinal lymph node and, heart. Congestion and consolidated areas with edema were seen in the examination of the lung. Histopathologically, neoplastic lymphoid cells were observed in intestine, lymph nodes and heart. In lung, vein lumens were almost completely occluded by mostly loose intimal fibrosis. Near affected veins, increasing in number of the capillaries was observed. While intimal fibrosis was detected by Masson's trichrome staining, the occluded veins were demonstrated by Verhoeff-van Gieson staining. In this case report, pathomorphological evaluation of PVOD was performed and as a result of clinicopathological examinations; PVOD, intestinal lymphoma, and cardiac metastasis were described in a male cat. [ABSTRACT FROM AUTHOR]

To date, there is no report on molecular characterisation of Myxobolus ichkeulensis in Mugil cephalus (Mugilidae) in Turkish marine waters. The aim of the current research was to provide the first molecular data on M. ichkeulensis from M. cephalus in Turkish Black Sea coasts. A total of 40 M. cephalus specimens were examined for the presence of M. ichkeulensis in the Turkish Black Sea coasts during January 2017 and April 2017. In the present study, M. ichkeulensis was identified based upon the myxospore morphology, host and tissue specificity, and SSU rRNA sequence. Phylogenetic analysis using neighbor-joining also revealed that our isolate (#GZP-2018-Samsun1) was clustered with other Myxobolus species that infect M. cephalus. The current study includes the morphological and histopathological descriptions and first molecular data on M. ichkeulensis in M. cephalus in Turkish marine waters. M. ichkeulensis was also reported from the M. cephalus in the coast of the Black Sea in Turkey for the first time. Moreover, our current genetic data was recorded as the new valid SSU rRNA sequence (MH374629) for M. ichkeulensis in the GenBank database. [ABSTRACT FROM AUTHOR]

The carcasses of three 1-year-old, Leghorn chickens were presented for necropsy; one of the chickens was told to have shown neurological signs before death. At necropsy, meningeal and cerebral hyperemia were observed in the carcass that had neurological symptoms, and diarrhea was evident in the other two chickens. Histopathological examination revealed nonsuppurative meningoencephalitis, myositis in skeletal and cardiac muscles, and focal nonsuppurative hepatitis, and nephritis. Bradyzoites and tachyzoites were present in the meninges and brain. The present case is the first report of Toxoplasma gondii infection in a chicken in Turkey. [ABSTRACT FROM AUTHOR]

Common magpies (Corvidae: Pica pica) distribute through rural and urban areas of Turkey. Because of their distribution in urbanised regions, magpies may have some potential infectious agents which may relate to domestic animals and humans. In this study, eight common magpies brought to the animal hospital in need of medical intervention were examined for endoparasites and bacteria in a one-year period. Additionally, histopathologic examinations with related organs were carried out along with endo-parasitical, cytological and microbial examination the following necropsy. As results of the necropsies, three parasite species including two helminths and one protozoan (Passerilepis sp., Brachylaima sp., Isospora rochalimai, respectively) were identified, while Staphylococcus xylosus, S. sciuri, Escherichia coli, Klebsiella pneumonia, Salmonella spp. were isolated after microbiological examination. Histopathology revealed that subacute focal mycotic pneumonia, chronic nonpurulent granulomatous gastroenteritis, verminous enteritis, and the presence of paratyphoid nodules in liver. Both of the parasites and bacteria are the first records for Turkey's helminth/bacterial fauna in wild birds. [ABSTRACT FROM AUTHOR]

The aim of this study is to determine C. parvum in small intestinal samples of lambs (up to 30 days old) with enteritis. For this aim, fresh intestinal smears prepared from 60 lambs (euthanized due to diarrhea) and stained with Modified Ziehl- Neelsen (MZN). In addition to these 60 fresh samples, 85 different small intestinal samples obtained from dead lambs (total 145 small intestinal samples) were embedded in paraffin for histopathology, immunofluorescence (IF) and immunohistochemistry (IHC). Slides were stained according to protocols (MZN, hematoxylin-eosin, IHC and IF). In MZN staining, 13.3% of smear samples were found positive for Cryptosporidium spp. Besides, C. parvum positivity was detected in the rate of 13.8% in IF and IHC stains. In the histopathologic examination, desquamation and necrosis of intestinal epithelium, villus atrophy, Cryptosporidium spp. along the surface of villus epithelium, hyperemia, mononuclear cellular infiltration, mitosis in crypt epithelium, intraepithelial lymphocytes, dilatation in the lymphatic canal and Eimeria spp. in some cases were observed in varying degree. In conclusion, IF, IHC and MZN stains were found effective in revealing cryptosporidiosis in lambs. [ABSTRACT FROM AUTHOR]

In this study the detection of Mycobacterium bovis with classical and molecular techniques and comparisons of the test results in the thirty slaughtered cattle positive to intradermal tuberculin test (PPD) was aimed. The classical methods include macroscopic examination of the organs, microscopic examination of tissues and blood samples that were stained by HE, ZN, immunohistochemistry and immunocytochemistry and PCR as molecular investigation. Characteristic tubercle development was observed in 26 animals' macroscopic examination yet 27 and 20 cases were found compatible with tuberculosis in HE and ZN respectively. Tuberculosis positive results were also obtained from lungs, mediastinal lymph nodes and liver via immunohistochemistry (IHC), PCRtissue, immunocytochemistry of the buffy coat (ICCbc) and PCR of the buffy coat (PCRbc) in 26, 18, 3 and 14 cows respectively. The agents were observed extracytoplasmically and intracytoplasmically in macrophages, giant cells, lymphocytes and fibrocytes in IHC. ICCbc positivity was observed within the cytoplasm of mononuclear leukocytes. Furthermore, the agent was not always detected via ZN staining, which verifies the necessity of ancillary tests. Among these methods IHC has revealed significant sensitivity compared to those of PCRbc and ICCbc (Mc nemar; p<0.05 ve 0.01 respectively), and exhibited compatibility with HE and ZN (Cohen kappa; p<0.001 and 0.05 respectively). These results confirm the fact that rather than one single golden technique, combination of various techniques is necessary for accurate and rapid diagnosis of bovine tuberculosis. It has also been concluded that further investigation is necessary to clarify the efficient routine usage of molecular techniques (PCRtissue/bc) in place of other diagnostic techniques. [ABSTRACT FROM AUTHOR]

Three male calves with a dark mass, hanging down on the skin at various parts of the body, were presented for clinical examination. All masses were present at the birth of the calves and their size increased with time. The masses were extirpated by a routine surgical operation and presented for histopathological examination. Tumour cells having cytoplasmic melanin granules showed a round or spindle-shaped nuclei. The mean mitotic rate was 5-7 at a high power field. Neoplastic melanocytes displayed an evident cytoplasmic immunolabeling to Melan A, HMB-45, S-100, NSE, and Vimentin. Tumour cells showed a nuclear positivity for Ki-67. Nuclear pleomorphism and a high mitotic rate along with the positive reaction of neoplastic cells to the melanocytic markers were indicative of melanoma. Nevertheless, neoplasms originating from melanocytes always need advanced imaging techniques along with histopathological and immunohistochemical examination while assessing biological behavior of the tumour. [ABSTRACT FROM AUTHOR]

In this study, juvenile common carp (Cyprinus carpio) were subjected to sublethal concentrations of the synthetic pyrethroids cypermethrin (2 µg/L) and cyphenothrin (5 µg/L) for 75 days. The toxic effects of these substances were analyzed pathomorphologically by conducting necropsies on 7 fish on days 15, 30, 45, 60, and 75. In addition, histopathological examination of the gonads was performed by exposing to 17β-estradiol (E2) (25 µ;g/L) as a positive control group to assess the endocrine disrupting effects of cypermethrin and cyphenothrin. Hyperemia, telangiectasia, epithelial lifting, and branchitis were observed in the gills of the fish from both treatment groups, and hemorrhage in the kidneys and hyperemia in the brain were also detected. Only 1 fish from each of the cypermethrin and E2 groups was observed to have degeneration of the testicles and edema of the ovary. One fish from the cyphenothrin group had degeneration of the testicles and another had oophoritis of the ovary. Based on the hyperemia and telangiectasia observed in the gills, it was concluded that both cypermethrin and cyphenothrin may lead to shortness of breath in these fish. However, only minor lesions were detected in the gonads; thus, there was no evidence that these synthetic pyrethroids affect the endocrine system. [ABSTRACT FROM AUTHOR]

The material of this study was, cytopathologic and histopathologic specimens obtained from 43 of dogs suffering from skin tumors. Samples were submitted by Ankara University, Faculty of Veterinary Medicine or private clinics of veterinary in Ankara. Cytologic specimens were prepared by fine needle aspiration biopsy (FNAB) technique and stained with May-Grünwald Giemsa (MGG). For histopatho logic examinations tissue samples were obtained by surgery. Samples were fixed in 10% neutral formalin solution and embedded in paraffin by routine procedures and stained with Hematoxylin-Eosin (H&E). Tumors were obtained from 29 male dogs and 14 female dogs. The age with highest incidence in dogs was between 8 and 15. The breeds of dogs more frequently affected were 14 terrier, 14 mongrel and 15 another different breeds. Localizations of tumors were in the followed regions, head, perianal regions and legs. Cytopathologically, 30 (69,76%) malignant epithelial tumors, 5 (11,62%) benign epithelial tumors, 6 (13,95%) suspect epithelial tumors and 2 (4,65%) few malignant epithelial tumors were diagnosed. Histopathological diagnosis determined 12 (27,9%) squoamous cell carcinoma, 9 (20,9%) hepatoid gland tumours, 7 (16,3%) basal cell carcinoma, 7 (16,3%) sweat gland tumours, 5 (11,6%) sebaceous gland tumours, 2 (4,7%) papilloma and 1 (2,3%) malignant pilomatricoma. The diagnoses were in agreement cytopathologically and histopatho logically in 86,05% of cases. The results obtained in the present study suggest that cytopatho logy is a practical tool for the early diagnosis of canine skin tumours. [ABSTRACT FROM AUTHOR]

In this study, cytological and histological findings of canine hepatoid gland tumors were evaluated for accuracy of cytologic diagnosis. Thirty two samples from dogs of different breed, sex and age were used in the study. Cytologic speciments were prepared by Fine Needle Aspiration Cytology (FNAC) and stained with May-Grünwald Giemsa (MGG). On the other hand, histopathologic tissue samples were obtained surgically. Samples were fixed in 10% neutral formalin solution and embedded in paraffin by routine procedures and stained with hematoxylin-eosin (H&E). In 84.37% of the samples taken from the 32 dogs included in the study, matching cytological and histopathological diagnoses were made, while 5 of the cases were misdiagnosed. Based on cytological examination, from 27 of the samples taken, 18 (66,7%) were diagnosed as Suspect adenoma, 6 (22,2%) as Suspect carcinoma and 3 (11,1%) as Suspect epithelioma. Based on histopathological examination, of the total 32 cases, 22 (68,7%) were diagnosed as Hepatoid gland adenoma (HGA), 7 (21,9%) as Hepatoid gland carcinoma (HGC) and 3 (9,4%) as Hepatoid gland epithelioma (HGE). These results suggest that the FNAC technique and cytological examination could be of use in the early diagnosis of canine hepatoid gland tumors. [ABSTRACT FROM AUTHOR]

Radioactive iodine (131I) is a known radionuclide which is used both for diagnostic and therapeutic purposes in the treatment of hyperthyroidism and thyroid cancer. Montelukast sodium (Cysteinyl leukotriene receptor-1 antagonist) is also a well-known antioxidant drug. This study aimed to evaluate the histopathological changes in rat livers at the third month following 131I treatment and the radioprotective effect of Montelukast sodium (ML) against I-related liver damage. Thirty female Wistar Albino rats were randomly divided into three groups as control group (n=10), untreated rats; second (RAI) group (n=10), oral radioiodine (111 MBq) administrated rats, and third (ML) group (n=10), oral radioiodine and ML administrated rats. In the third group, ML administration was started 3 days before and ended 10 days after RAI administration. In the third month of radioiodine (RAI) administration, the animals were decapitated and the livers were removed for histopathological examination. The histopathologic data were evaluated comparatively by using Mann Whitney U and Fisher's Exact Tests Differences were determined in all the parameters in terms of intensity between the control and 131I groups, which showed the harmful effects of 131I. In the comparison of the 131I and ML groups, hyperemia was determined respectively 80% to 40%, the presence of inflammatory cells 70% to 60% and capsule thickening 70% to 40%. Montelukast sodium was observed to have a protective effect especially on hyperemia and capsule thickening. According to the study results, radioactive iodine (131I) treatment seems to cause morphological damage to the rat liver, and Montelukast sodium effectively protects the liver against damage. [ABSTRACT FROM AUTHOR]

In the case, calcinosis circumscripta are described with macroscopical and microscopical findings on proximal of right limb in a 2.5 years old, male Rotweiller dog. Macroscopically, all masses were covered with skin and had firm. The cut sections were seen lobullary and composed of well defined areas and vicious and white fluid. Microscopically, it was detected that numbered of restricted areas, where the center had calcification in dark blue stained as haematoxylin eosin (HE) and red stained with Alizerine red S., connective tissue and mononuclear cell infiltration consisting of macrophages, lymphocytes and plasma cells and giant cells. [ABSTRACT FROM AUTHOR]

In the case, diffuse malignant seminoma was pathologically described in both testicles of 6 years old, male Terrier. Testicles had firmness consistency and multilobullary appearence. On cut sections, a wide brownish-black colored and haemorragic area were restricted by gray colored necrotic areas. Microscopically, multiple masses in different size which composed of polihedral shaped neoplastic cells that had vesicular nuclei and basophilic cytoplasm. [ABSTRACT FROM AUTHOR]