1. Improving the Quality and Reproducibility of Flow Cytometry in the Lung. An Official American Thoracic Society Workshop Report
- Author
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Hideki Nakano, Alexander V. Misharin, William J. Zacharias, Susanne Herold, Suchitra Swaminathan, Benjamin D. Singer, Elizabeth F. Redente, Claudia Jakubzick, Ryan Duggan, William J. Janssen, Anne I. Sperling, Christine M. Freeman, Jeffrey L. Curtis, Ryan R. Brinkman, Robert M. Tighe, and Yen-Rei A. Yu
- Subjects
0301 basic medicine ,Pulmonary and Respiratory Medicine ,Lung Diseases ,medicine.medical_specialty ,media_common.quotation_subject ,Clinical Biochemistry ,Apoptosis ,Cell Separation ,lung biology ,Flow cytometry ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Animals ,Humans ,Quality (business) ,Myeloid Cells ,Intensive care medicine ,Molecular Biology ,Pulmonary flow ,reproducibility ,Lung ,Organ system ,Societies, Medical ,media_common ,American Thoracic Society Documents ,Reproducibility ,medicine.diagnostic_test ,business.industry ,flow cytometry ,Reproducibility of Results ,Cell Biology ,Limiting ,Congresses as Topic ,United States ,030104 developmental biology ,medicine.anatomical_structure ,Phenotype ,030228 respiratory system ,Practice Guidelines as Topic ,cells ,business ,Cytometry - Abstract
Defining responses of the structural and immune cells in biologic systems is critically important to understanding disease states and responses to injury. This requires accurate and sensitive methods to define cell types in organ systems. The principal method to delineate the cell populations involved in these processes is flow cytometry. Although researchers increasingly use flow cytometry, technical challenges can affect its accuracy and reproducibility, thus significantly limiting scientific advancements. This challenge is particularly critical to lung immunology, as the lung is readily accessible and therefore used in preclinical and clinical studies to define potential therapeutics. Given the importance of flow cytometry in pulmonary research, the American Thoracic Society convened a working group to highlight issues and technical challenges to the performance of high-quality pulmonary flow cytometry, with a goal of improving its quality and reproducibility.
- Published
- 2019