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Start Over Author "Lee John S" Publisher american society of tropical medicine and hygiene
8 results on '"Lee John S"'

2. Field Detection of Tembusu Virus in Western Thailand by RT-PCR and Vector Competence Determination of Select Culex Mosquitoes for Transmission of the Virus

Author

O'Guinn, Monica L., primary, Coleman, Russell E., additional, Endy, Timothy P., additional, Lee, John S., additional, Turell, Michael J., additional, Kengluecha, Ampornpan, additional, Jaichapor, Boonsong, additional, Kankaew, Prasan, additional, Jones, James W., additional, and Miller, R. Scott, additional

Published
2013
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3. Evaluation of a Field-Portable DNA Microarray Platform and Nucleic Acid Amplification Strategies for the Detection of Arboviruses, Arthropods, and Bloodmeals

Author

Grubaugh, Nathan D., primary, Kengluecha, Ampornpan, additional, Lee, John S., additional, Pisarcik, Sarah E., additional, O'Guinn, Monica L., additional, Melanson, Vanessa R., additional, Jaichapor, Boonsong, additional, Petz, Lawrence N., additional, McMenamy, Scott S., additional, Long, Lewis S., additional, and Turell, Michael J., additional

Published
2013
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4. Rift Valley Fever Virus Epidemic in Kenya, 2006/2007: The Entomologic Investigations

Author

Sang, Rosemary, primary, Hanafi, Hanafi, additional, Warigia, Marion, additional, Breiman, Robert F., additional, Godsey, Marvin, additional, Hoel, David, additional, Lutomiah, Joel, additional, Koka, Hellen, additional, O'Guinn, Monica, additional, Miller, Barry, additional, Ochieng, Caroline, additional, Lee, John S., additional, Schnabel, David, additional, Kioko, Elizabeth, additional, and Richardson, Jason, additional

Published
2010
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8. Field detection of eastern equine encephalitis virus in the Amazon Basin region of Peru using reverse transcription-polymerase chain reaction adapted for field identification of arthropod-borne pathogens.

Author

O'Guinn ML, Lee JS, Kondig JP, Fernandez R, and Carbajal F

Subjects
Animals, DNA, Complementary chemistry, DNA, Complementary metabolism, Encephalitis Virus, Eastern Equine classification, Encephalitis Virus, Eastern Equine genetics, Peru, Phylogeny, RNA, Viral genetics, RNA, Viral isolation & purification, Culex virology, Encephalitis Virus, Eastern Equine isolation & purification, Insect Vectors virology, Ochlerotatus virology, Reverse Transcriptase Polymerase Chain Reaction methods
Abstract

In support of efforts to develop rapid diagnostic assays for use in the field, reverse transcription-polymerase chain reaction (RT-PCR) assays were developed to detect arboviruses circulating in the Amazon Basin region of Peru. Previous knowledge of arthropod/pathogen relationships allowed a focused evaluation to be conducted in November 2000 that assessed the feasibility and reliability of a mobile, rapid, field-expedient RT-PCR diagnostic system aimed at detecting eastern equine encephalitis virus (EEEV) in Culex (Melanoconion) pedroi mosquitoes. Modifications were made to a commercially available mobile molecular laboratory kit and assay procedures were tailored for use under harsh environmental conditions with field-collected and field-processed mosquitoes. From CO2 baited mosquito light traps, 3,227 Cx. (Mel.) pedroi mosquitoes were collected and sorted into 117 pools. The pools were processed and assayed in the field by RT-PCR and five of those pools were found positive for EEEV. Laboratory sequence analysis confirmed the presence of two distinct subtypes of EEEV.

Published
2004

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