1. Comparison of Thick Films, in Vitro Culture and DNA Hybridization Probes for Detecting Plasmodium falciparum Malaria
- Author
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McLaughlin Gl, Dyann F. Wirth, Barker Rj, Chulay Jd, Zolg Jw, and David E. Lanar
- Subjects
Plasmodium falciparum ,Parasitemia ,Azure Stains ,Giemsa stain ,Virology ,parasitic diseases ,medicine ,Animals ,Humans ,False Positive Reactions ,biology ,DNA–DNA hybridization ,Hybridization probe ,Nucleic Acid Hybridization ,DNA ,medicine.disease ,biology.organism_classification ,In vitro ,Malaria ,Blood ,Infectious Diseases ,Induced malaria ,Parasitology ,DNA Probes - Abstract
Using blood from volunteers with sporozoite induced malaria, a comparison was made of the sensitivity and specificity of Giemsa stained thick film examination, in vitro culture, and 4 different DNA probes for detecting parasitemia. Between 9 and 13 days after sporozoite inoculation, patent parasitemia (4-550 parasites/microliters) was detected by thick film examination of 0.5 microliters blood in 7 volunteers. Cultures of 1 ml blood obtained 7 days after sporozoite inoculation were positive in all volunteers who eventually developed patent parasitemia. The DNA hybridization probes detected parasites in only 5-28% of smear- or culture-positive samples.
- Published
- 1989
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