5 results on '"Zhang, Dajian"'
Search Results
2. OsmiR396d-Regulated OsGRFs Function in Floral Organogenesis in Rice through Binding to Their Targets OsJMJ706 and OsCR41[W][OPEN]
- Author
-
Liu, Huanhuan, Guo, Siyi, Xu, Yunyuan, Li, Chunhua, Zhang, Zeyong, Zhang, Dajian, Xu, Shujuan, Zhang, Cui, and Chong, Kang
- Subjects
Base Sequence ,Transcription, Genetic ,Organogenesis ,Molecular Sequence Data ,Oryza ,Articles ,Flowers ,Plants, Genetically Modified ,MicroRNAs ,Phenotype ,Gene Expression Regulation, Plant ,Gene Knockdown Techniques ,Mutation ,Protein Interaction Mapping ,Amino Acid Sequence ,Inflorescence ,Genome, Plant ,In Situ Hybridization ,Plant Proteins ,Protein Binding - Abstract
Inflorescence and spikelet development determine grain yields in cereals. Although multiple genes are known to be involved in the regulation of floral organogenesis, the underlying molecular network remains unclear in cereals. Here, we report that the rice (Oryza sativa) microRNA396d (OsmiR396d) and its Os Growth Regulating Factor (OsGRF) targets, together with Os Growth Regulating Factor-Interacting Factor1 (OsGIF1), are involved in the regulation of floral organ development through the rice JMJD2 family jmjC gene 706 (OsJMJ706) and crinkly4 receptor-like kinase (OsCR4). Transgenic knockdown lines of OsGRF6, a predicted target of OsmiR396d, and overexpression lines of OsmiR396d showed similar defects in floral organ development, including open husks, long sterile lemmas, and altered floral organ morphology. These defects were almost completely rescued by overexpression of OsGRF6. OsGRF6 and its ortholog OsGRF10 were the most highly expressed OsGRF family members in young inflorescences, and the grf6/grf10 double mutant displayed abnormal florets. OsGRF6/OsGRF10 localized to the nucleus, and electrophoretic mobility shift assays revealed that both OsGRF6 and OsGRF10 bind the GA response element in the promoters of OsJMJ706 and OsCR4, which were reported to participate in the regulation of floral organ development. In addition, OsGRF6 and OsGRF10 could transactivate OsJMJ706 and OsCR4, an activity that was enhanced in the presence of OsGIF1, which can bind both OsGRF6 and OsGRF10. Together, our results suggest that OsmiR396d regulates the expression of OsGRF genes, which function with OsGIF1 in floret development through targeting of JMJ706 and OsCR4. This work thus reveals a microRNA-mediated regulation module for controlling spikelet development in rice.
- Published
- 2014
3. The F-Box Protein OsFBK12 Targets OsSAMS1 for Degradation and Affects Pleiotropic Phenotypes, Including Leaf Senescence, in Rice1[W][OPEN]
- Author
-
Chen, Yuan, Xu, Yunyuan, Luo, Wei, Li, Wenxuan, Chen, Na, Zhang, Dajian, and Chong, Kang
- Subjects
Cell Nucleus ,F-Box Proteins ,food and beverages ,Cell Count ,Genetic Pleiotropy ,Germination ,Oryza ,Organ Size ,Plants, Genetically Modified ,Models, Biological ,Article ,Endosperm ,Plant Leaves ,Phenotype ,Gene Expression Regulation, Plant ,Gene Knockdown Techniques ,Plant Cells ,Proteolysis ,Seeds ,sense organs ,Cell Size ,Plant Proteins ,Protein Binding - Abstract
Leaf senescence is related to the grain-filling rate and grain weight in cereals. Many components involved in senescence regulation at either the genetic or physiological level are known. However, less is known about molecular regulation mechanisms. Here, we report that OsFBK12 (an F-box protein containing a Kelch repeat motif) interacts with S-ADENOSYL-l-METHIONINE SYNTHETASE1 (SAMS1) to regulate leaf senescence and seed size as well as grain number in rice (Oryza sativa). Yeast two-hybrid, pull-down, and bimolecular fluorescence complementation assays indicate that OsFBK12 interacts with Oryza sativa S-PHASE KINASE-ASSOCIATED PROTEIN1-LIKE PROTEIN and with OsSAMS1. Biochemical and physiological data showed that OsFBK12 targets OsSAMS1 for degradation. OsFBK12-RNA interference lines and OsSAMS1 overexpression lines showed increased ethylene levels, while OsFBK12-OX lines and OsSAMS1-RNA interference plants exhibited decreased ethylene. Phenotypically, overexpression of OsFBK12 led to a delay in leaf senescence and germination and increased seed size, whereas knockdown lines of either OsFBK12 or OsSAMS1 promoted the senescence program. Our results suggest that OsFBK12 is involved in the 26S proteasome pathway by interacting with Oryza sativa S-PHASE KINASE-ASSOCIATED PROTEIN1-LIKE PROTEIN and that it targets the substrate OsSAMS1 for degradation, triggering changes in ethylene levels for the regulation of leaf senescence and grain size. These data have potential applications in the molecular breeding of rice.
- Published
- 2013
4. OsmiR396d-regulated OsGRFs function in floral organogenesis in rice through binding to their targets OsJMJ706 and OsCR4.
- Author
-
Liu H, Guo S, Xu Y, Li C, Zhang Z, Zhang D, Xu S, Zhang C, and Chong K
- Subjects
- Amino Acid Sequence, Base Sequence, Gene Expression Regulation, Plant, Gene Knockdown Techniques, Genome, Plant, In Situ Hybridization, Inflorescence genetics, Inflorescence growth & development, MicroRNAs genetics, Molecular Sequence Data, Mutation, Phenotype, Plant Proteins chemistry, Plant Proteins genetics, Plants, Genetically Modified, Protein Binding, Protein Interaction Mapping, Transcription, Genetic, Flowers genetics, Flowers growth & development, MicroRNAs metabolism, Organogenesis genetics, Oryza genetics, Oryza growth & development, Plant Proteins metabolism
- Abstract
Inflorescence and spikelet development determine grain yields in cereals. Although multiple genes are known to be involved in the regulation of floral organogenesis, the underlying molecular network remains unclear in cereals. Here, we report that the rice (Oryza sativa) microRNA396d (OsmiR396d) and its Os Growth Regulating Factor (OsGRF) targets, together with Os Growth Regulating Factor-Interacting Factor1 (OsGIF1), are involved in the regulation of floral organ development through the rice JMJD2 family jmjC gene 706 (OsJMJ706) and crinkly4 receptor-like kinase (OsCR4). Transgenic knockdown lines of OsGRF6, a predicted target of OsmiR396d, and overexpression lines of OsmiR396d showed similar defects in floral organ development, including open husks, long sterile lemmas, and altered floral organ morphology. These defects were almost completely rescued by overexpression of OsGRF6. OsGRF6 and its ortholog OsGRF10 were the most highly expressed OsGRF family members in young inflorescences, and the grf6/grf10 double mutant displayed abnormal florets. OsGRF6/OsGRF10 localized to the nucleus, and electrophoretic mobility shift assays revealed that both OsGRF6 and OsGRF10 bind the GA response element in the promoters of OsJMJ706 and OsCR4, which were reported to participate in the regulation of floral organ development. In addition, OsGRF6 and OsGRF10 could transactivate OsJMJ706 and OsCR4, an activity that was enhanced in the presence of OsGIF1, which can bind both OsGRF6 and OsGRF10. Together, our results suggest that OsmiR396d regulates the expression of OsGRF genes, which function with OsGIF1 in floret development through targeting of JMJ706 and OsCR4. This work thus reveals a microRNA-mediated regulation module for controlling spikelet development in rice.
- Published
- 2014
- Full Text
- View/download PDF
5. The F-box protein OsFBK12 targets OsSAMS1 for degradation and affects pleiotropic phenotypes, including leaf senescence, in rice.
- Author
-
Chen Y, Xu Y, Luo W, Li W, Chen N, Zhang D, and Chong K
- Subjects
- Cell Count, Cell Nucleus metabolism, Cell Size, Endosperm cytology, Endosperm metabolism, Endosperm ultrastructure, F-Box Proteins genetics, Gene Expression Regulation, Plant, Gene Knockdown Techniques, Germination, Models, Biological, Organ Size genetics, Oryza genetics, Oryza metabolism, Oryza ultrastructure, Phenotype, Plant Cells metabolism, Plant Proteins genetics, Plants, Genetically Modified, Protein Binding, Seeds anatomy & histology, F-Box Proteins metabolism, Genetic Pleiotropy, Oryza growth & development, Plant Leaves growth & development, Plant Leaves metabolism, Plant Proteins metabolism, Proteolysis
- Abstract
Leaf senescence is related to the grain-filling rate and grain weight in cereals. Many components involved in senescence regulation at either the genetic or physiological level are known. However, less is known about molecular regulation mechanisms. Here, we report that OsFBK12 (an F-box protein containing a Kelch repeat motif) interacts with S-ADENOSYL-l-METHIONINE SYNTHETASE1 (SAMS1) to regulate leaf senescence and seed size as well as grain number in rice (Oryza sativa). Yeast two-hybrid, pull-down, and bimolecular fluorescence complementation assays indicate that OsFBK12 interacts with Oryza sativa S-PHASE KINASE-ASSOCIATED PROTEIN1-LIKE PROTEIN and with OsSAMS1. Biochemical and physiological data showed that OsFBK12 targets OsSAMS1 for degradation. OsFBK12-RNA interference lines and OsSAMS1 overexpression lines showed increased ethylene levels, while OsFBK12-OX lines and OsSAMS1-RNA interference plants exhibited decreased ethylene. Phenotypically, overexpression of OsFBK12 led to a delay in leaf senescence and germination and increased seed size, whereas knockdown lines of either OsFBK12 or OsSAMS1 promoted the senescence program. Our results suggest that OsFBK12 is involved in the 26S proteasome pathway by interacting with Oryza sativa S-PHASE KINASE-ASSOCIATED PROTEIN1-LIKE PROTEIN and that it targets the substrate OsSAMS1 for degradation, triggering changes in ethylene levels for the regulation of leaf senescence and grain size. These data have potential applications in the molecular breeding of rice.
- Published
- 2013
- Full Text
- View/download PDF
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