25 results on '"Kim, Woo Taek"'
Search Results
2. Arabidopsis PUB22 and PUB23 are homologous U-box E3 ubiquitin ligases that play combinatory roles in response to drought stress
- Author
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Cho, Seok Keun, Ryu, Moon Young, Song, Charlotte, Kwak, June M., and Kim, Woo Taek
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Ubiquitin -- Reports ,Abscisic acid -- Reports ,Arabidopsis thaliana -- Reports ,Aquatic resources -- Reports ,Ligases -- Reports ,Biological sciences ,Science and technology - Published
- 2008
3. Further characterization of a rice AGL12 group MADS-box gene, OsMADS26
- Author
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Lee, Shinyoung, Woo, Young-Min, Ryu, Sung-Il, Shin, Young-Duck, Kim, Woo Taek, Park, Ky Young, Lee, In-Jung, and An, Gynheung
- Subjects
Plant genetics -- Genetic aspects ,Plant genetics -- Chemical properties ,Rice -- Genetic aspects ,Rice -- Chemical properties ,Biological sciences ,Science and technology - Published
- 2008
4. Suppression of RICE TELOMERE BINDING PROTEIN1 results in severe and gradual developmental defects accompanied by genome instability in rice
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Hong, Jong-Pil, Byun, Mi Young, Koo, Dal-Hoe, An, Kyungsook, Bang, Jae-Wook, Chung, In Kwon, An, Gynheung, and Kim, Woo Taek
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Rice -- Physiological aspects ,Rice -- Genetic aspects ,Telomeres -- Properties ,Binding proteins -- Properties ,Binding proteins -- Control ,Genomics -- Research ,Plant physiology -- Research ,Mutation (Biology) -- Influence ,Biological sciences ,Science and technology - Published
- 2007
5. Heterologous expression and molecular and cellular characterization of CaPUB1 encoding a hot pepper U-box E3 ubiquitin ligase homolog (1)[C]
- Author
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Cho, Seok Keun, Chung, Hoo Sun, Ryu, Moon Young Park, Mi Jin, Lee, Myeong Min, Bahk, Young-Yil, Kim, Jungmook, Pai, Hyun Sook, and Kim, Woo Taek
- Subjects
Plant cells and tissues -- Research ,Eukaryotes -- Research ,Gene expression -- Research ,Ubiquitin-proteasome system -- Research ,Biological sciences ,Science and technology - Published
- 2006
6. Mitochondria-associated hexokinases play a role in the control of programmed cell death in nicotiana benthamiana ([W])
- Author
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Kim, Moonil, Lim, Jeong-Hwa, Ahn, Chang Sook, Park, Kyoungsook, Kim, Gyung Tae, Kim, Woo Taek, and Pai, Hyun-Sook
- Subjects
Apoptosis -- Research ,Hexokinases -- Research ,Tobacco (Plant) -- Research ,Biological sciences ,Science and technology - Published
- 2006
7. Perturbation of NgTRF1 expression induces apoptosis-like cell death in tobacco BY-2 cells and implicates NgTRF1 in the control of telomere length and stability
- Author
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Yang, Seong Wook, Kim, Sung Keun, and Kim, Woo Taek
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Telomeres -- Structure ,Telomeres -- Reorganization and restructuring ,Apoptosis -- Research ,Company restructuring/company reorganization ,Company organization ,Biological sciences ,Science and technology - Published
- 2004
8. The pepper transcription factor CaPF1 confers pathogen and freezing tolerance in Arabidopsis (1)
- Author
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Yi, So Young, Kim, Jee-Hyub, Joung, Young-Hee, Lee, Sanghyeob, Kim, Woo-Taek, Yu, Seung Hun, and Choi, Doil
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Arabidopsis thaliana -- Research ,Arabidopsis thaliana -- Genetic aspects ,Gene expression -- Research ,Genetically modified plants -- Research ,Messenger RNA -- Research ,Pathogenic microorganisms -- Research ,Pepper -- Research ,Biological sciences ,Science and technology - Published
- 2004
9. Capsicum annuum tobacco mosaic virus-induced clone 1 expression perturbation alters the plant's response to ethylene and interferes with the redox homeostasis (1)
- Author
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Shin, Ryoung, An, Jong-Min, Park, Chang-Jin, Kim, Young Jin, Joo, Sunjoo, Kim, Woo Taek, and Paek, Kyung-Hee
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Tobacco mosaic virus -- Genetic aspects ,Sweet peppers -- Diseases and pests ,Sweet peppers -- Genetic aspects ,Plant immunology -- Genetic aspects ,Biological sciences ,Science and technology - Published
- 2004
10. Generation and analysis of end sequence database for T-DNA tagging lines in rice (1)
- Author
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An, Suyoung, Park, Sunhee, Jeong, Dong-Hoon, Lee, Dong-Yeon, Kang, Hong-Gyu, Yu, Jung-Hwa, Hur, Junghe, Kim, Sung-Ryul, Kim, Young-Hea, Lee, Miok, Han, Soonki, Kim, Soo-Jin, Yang, Jungwon, Kim, Eunjoo, Wi, Soo Jin, Chung, Hoo Sun, Hong, Jong-Pil, Choe, Vitnary, Lee, Hak-Kyung, Choi, Jung-Hee, Nam, Jongmin, Kim, Seong-Ryong, Park, Phun-Bum, Park, Ky Young, Kim, Woo Taek, Choe, Sunghwa, Lee, Chin-Bum, and An, Gynheung
- Subjects
Rice -- Genetic aspects ,Mutagenesis -- Analysis ,DNA -- Research ,Biological sciences ,Science and technology - Published
- 2003
11. Molecular and biochemical characterization of VR-EILs encoding mung bean ETHYLENE INSENSITIVE3-LIKE proteins (1)
- Author
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Lee, Jae-Hoon and Kim, Woo Taek
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Beans -- Genetic aspects ,Legumes -- Genetic aspects ,Mimosaceae -- Genetic aspects ,Plant molecular genetics -- Research ,Polymerase chain reaction -- Research ,Ethylene -- Chemical properties ,Biological sciences ,Science and technology - Published
- 2003
12. Arabidopsis Small Rubber Particle Protein Homolog SRPs Play Dual Roles as Positive Factors for Tissue Growth and Development and in Drought Stress Responses1[OPEN]
- Author
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Kim, Eun Yu, Park, Ki Youl, Seo, Young Sam, and Kim, Woo Taek
- Subjects
Arabidopsis ,Plant Roots ,Polymerization ,Plant Growth Regulators ,Cell Wall ,Gene Expression Regulation, Plant ,Stress, Physiological ,Tobacco ,Amino Acid Sequence ,Cell Proliferation ,Plant Proteins ,Arabidopsis Proteins ,technology, industry, and agriculture ,food and beverages ,Gene Expression Regulation, Developmental ,Articles ,Lipid Droplets ,Antigens, Plant ,Plants, Genetically Modified ,Droughts ,Plant Leaves ,Seedlings ,Mutation ,Seeds ,psychological phenomena and processes ,Abscisic Acid - Abstract
Lipid droplets (LDs) act as repositories for fatty acids and sterols, which are used for various cellular processes such as energy production and membrane and hormone synthesis. LD-associated proteins play important roles in seed development and germination, but their functions in postgermination growth are not well understood. Arabidopsis (Arabidopsis thaliana) contains three SRP homologs (SRP1, SRP2, and SRP3) that share sequence identities with small rubber particle proteins of the rubber tree (Hevea brasiliensis). In this report, the possible cellular roles of SRPs in postgermination growth and the drought tolerance response were investigated. Arabidopsis SRPs appeared to be LD-associated proteins and displayed polymerization properties in vivo and in vitro. SRP-overexpressing transgenic Arabidopsis plants (35S:SRP1, 35S:SRP2, and 35S:SRP3) exhibited higher vegetative and reproductive growth and markedly better tolerance to drought stress than wild-type Arabidopsis. In addition, constitutive over-expression of SRPs resulted in increased numbers of large LDs in postgermination seedlings. In contrast, single (srp1, 35S:SRP2-RNAi, and srp3) and triple (35S:SRP2-RNAi/srp1srp3) loss-of-function mutant lines exhibited the opposite phenotypes. Our results suggest that Arabidopsis SRPs play dual roles as positive factors in postgermination growth and the drought stress tolerance response. The possible relationships between LD-associated proteins and the drought stress response are discussed.
- Published
- 2016
13. Roles of Four Arabidopsis U-Box E3 Ubiquitin Ligases in Negative Regulation of Abscisic Acid-Mediated Drought Stress Responses1[C][W][OA]
- Author
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Seo, Dong Hye, Ryu, Moon Young, Jammes, Fabien, Hwang, Jae Hwan, Turek, Michelle, Kang, Bin Goo, Kwak, June M., and Kim, Woo Taek
- Subjects
Chlorophyll ,Arabidopsis Proteins ,Ubiquitin-Protein Ligases ,fungi ,Genetic Complementation Test ,Molecular Sequence Data ,Arabidopsis ,food and beverages ,Hydrogen Peroxide ,Genes, Plant ,Plants, Genetically Modified ,Adaptation, Physiological ,Plant Roots ,Systems Biology, Molecular Biology, and Gene Regulation ,Droughts ,Phenotype ,Gene Expression Regulation, Plant ,Stress, Physiological ,Plant Stomata ,Calcium ,Mannitol ,Amino Acid Sequence ,Abscisic Acid ,Signal Transduction - Abstract
AtPUB18 and AtPUB19 are homologous U-box E3 ubiquitin ligases in Arabidopsis (Arabidopsis thaliana). AtPUB19 is a negative regulator of abscisic acid (ABA)-mediated drought responses, whereas the role of AtPUB18 in drought responses is unknown. Here, loss-of-function and overexpression tests identified AtPUB18 as a negative regulator in ABA-mediated stomatal closure and water stress responses. The atpub18-2atpub19-3 double mutant line displayed more sensitivity to ABA and enhanced drought tolerance than each single mutant plant; therefore, AtPUB18 and AtPUB19 are agonistic. Stomatal closure of the atpub18-2atpub19-3 mutant was hypersensitive to hydrogen peroxide (H(2)O(2)) but not to calcium, suggesting that AtPUB18 and AtPUB19 exert negative effects on the ABA signaling pathway downstream of H(2)O(2) and upstream of calcium. AtPUB22 and AtPUB23 are other U-box E3 negative regulators of drought responses. Although atpub22atpub23 was more tolerant to drought stress relative to wild-type plants, its ABA-mediated stomatal movements were highly similar to those of wild-type plants. The atpub18-2atpub19-3atpub22atpub23 quadruple mutant exhibited enhanced tolerance to drought stress as compared with each atpub18-2atpub19-3 and atpub22atpub23 double mutant progeny; however, its stomatal behavior was almost identical to the atpub18-2atpub19-3 double mutant in the presence of ABA, H(2)O(2), and calcium. Overexpression of AtPUB18 and AtPUB19 in atpub22atpub23 effectively hindered ABA-dependent stomatal closure, but overexpression of AtPUB22 and AtPUB23 in atpub18-2atpub19-3 did not inhibit ABA-enhanced stomatal closure, highlighting their ABA-independent roles. Overall, these results suggest that AtPUB18 has a linked function with AtPUB19, but is independent from AtPUB22 and AtPUB23, in negative regulation of ABA-mediated drought stress responses.
- Published
- 2012
14. The Arabidopsis C3H2C3-Type RING E3 Ubiquitin Ligase AtAIRP1 Is a Positive Regulator of an Abscisic Acid-Dependent Response to Drought Stress1[C][W][OA]
- Author
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Ryu, Moon Young, Cho, Seok Keun, and Kim, Woo Taek
- Subjects
DNA, Bacterial ,Sequence Homology, Amino Acid ,Arabidopsis Proteins ,Ubiquitin-Protein Ligases ,fungi ,Molecular Sequence Data ,Arabidopsis ,food and beverages ,Systems Biology, Molecular Biology, and Gene Regulation ,Droughts ,Up-Regulation ,Stress, Physiological ,Mutation ,Amino Acid Sequence ,Abscisic Acid - Abstract
Ubiquitination is a eukaryotic posttranslational protein modification that is mediated by the cascade of E1, E2, and E3 ubiquitin (Ub) ligases and is involved in regulating numerous cellular functions. In this study, we obtained 100 different Arabidopsis (Arabidopsis thaliana) T-DNA insertion mutant plants in which RING E3 Ub ligase genes were suppressed and monitored their phenotypes in the presence of exogenous abscisic acid (ABA), a plant stress hormone. One of these loss-of-function mutants displayed ABA-insensitive phenotypes at the germination stage and was named atairp1 (for Arabidopsis ABA-insensitive RING protein 1). AtAIRP1 encodes a cytosolic protein containing a single C3H2C3-type RING motif with in vitro E3 Ub ligase activity. AtAIRP1 was significantly induced by ABA and drought stress. In contrast to atairp1 mutant plants, AtAIRP1-overexpressing transgenic plants (35S:AtAIRP1-sGFP) were hypersensitive to exogenous ABA in terms of radicle emergence, cotyledon development, root elongation, and stomatal closure. Ectopic expression of AtAIRP1-sGFP in atairp1 effectively rescued the loss-of-function ABA-insensitive phenotype. Both 35S:AtAIRP1-sGFP and atairp1/35S:AtAIRP1-sGFP plants accumulated higher amounts of hydrogen peroxide in response to exogenous ABA than did wild-type and atairp1 mutant plants. AtAIRP1 overexpressors were markedly tolerant to severe drought stress, as opposed to atairp1, which was highly susceptible. The levels of drought stress-related genes and basic leucine zipper transcription factor genes were up-regulated in the 35S:AtAIRP1-sGFP lines relative to wild-type and atairp1 mutant plants in response to ABA. Overall, these results suggest that AtAIRP1, a C3H2C3-type RING E3 Ub ligase, is a positive regulator in the Arabidopsis ABA-dependent drought response.
- Published
- 2010
15. The Pepper Transcription Factor CaPF1 Confers Pathogen and Freezing Tolerance in Arabidopsis1
- Author
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Yi, So Young, Kim, Jee-Hyub, Joung, Young-Hee, Lee, Sanghyeob, Kim, Woo-Taek, Yu, Seung Hun, and Choi, Doil
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Binding Sites ,Base Sequence ,DNA, Plant ,Sequence Homology, Amino Acid ,fungi ,Molecular Sequence Data ,Arabidopsis ,food and beverages ,Gene Expression ,Pseudomonas syringae ,Genes, Plant ,Plants, Genetically Modified ,Recombinant Proteins ,RNA, Plant ,Freezing ,Tobacco ,Tissue Distribution ,Amino Acid Sequence ,RNA, Messenger ,Capsicum ,Phylogeny ,Research Article ,Transcription Factors - Abstract
An ERF/AP2-type transcription factor (CaPF1) was isolated by differential-display reverse transcription-PCR, following inoculation of the soybean pustule pathogen Xanthomonas axonopodis pv glycines 8ra, which induces hypersensitive response in pepper (Capsicum annuum) leaves. CaPF1 mRNA was induced under conditions of biotic and abiotic stress. Higher levels of CaPF1 transcripts were observed in disease-resistant tissue compared with susceptible tissue. CaPF1 expression was additionally induced using various treatment regimes, including ethephon, methyl jasmonate, and cold stress. To determine the role of CaPF1 in plants, transgenic Arabidopsis and tobacco (Nicotiana tabacum) plants expressing higher levels of CaPF1 were generated. Gene expression analyses of transgenic Arabidopsis and tobacco revealed that the CaPF1 level in transgenic plants affects expression of genes that contain either a GCC or a CRT/DRE box in their promoter regions. Furthermore, transgenic Arabidopsis plants expressing CaPF1 displayed tolerance against freezing temperatures and enhanced resistance to Pseudomonas syringae pv tomato DC3000. Disease tolerance was additionally observed in CaPF1 transgenic tobacco plants. The results collectively indicate that CaPF1 is an ERF/AP2 transcription factor in hot pepper plants that may play dual roles in response to biotic and abiotic stress in plants.
- Published
- 2004
16. E3 ligase AtAIRP5/GARU regulates drought stress response by stimulating SERINE CARBOXYPEPTIDASE-LIKE1 turnover.
- Author
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Cho NH, Woo OG, Kim EY, Park K, Seo DH, Yu SG, Choi YA, Lee JH, Lee JH, and Kim WT
- Subjects
- Abscisic Acid metabolism, Abscisic Acid pharmacology, Amino Acid Sequence, Carboxypeptidases, Droughts, Gene Expression Regulation, Plant, Plants, Genetically Modified metabolism, Stress, Physiological genetics, Ubiquitin-Protein Ligases genetics, Ubiquitin-Protein Ligases metabolism, Arabidopsis metabolism, Arabidopsis Proteins genetics, Arabidopsis Proteins metabolism
- Abstract
Ubiquitination is a major mechanism of eukaryotic posttranslational protein turnover that has been implicated in abscisic acid (ABA)-mediated drought stress response. Here, we isolated T-DNA insertion mutant lines in which ABA-insensitive RING protein 5 (AtAIRP5) was suppressed, resulting in hyposensitive ABA-mediated germination compared to wild-type Arabidopsis (Arabidopsis thaliana) plants. A homology search revealed that AtAIRP5 is identical to gibberellin (GA) receptor RING E3 ubiquitin (Ub) ligase (GARU), which downregulates GA signaling by degrading the GA receptor GID1, and thus AtAIRP5 was renamed AtAIRP5/GARU. The atairp5/garu knockout progeny were impaired in ABA-dependent stomatal closure and were markedly more susceptible to drought stress than wild-type plants, indicating a positive role for AtAIRP5/GARU in the ABA-mediated drought stress response. Yeast two-hybrid, pull-down, target ubiquitination, and in vitro and in planta degradation assays identified serine carboxypeptidase-like1 (AtSCPL1), which belongs to the clade 1A AtSCPL family, as a ubiquitinated target protein of AtAIRP5/GARU. atscpl1 single and atairp5/garu-1 atscpl1-2 double mutant plants were more tolerant to drought stress than wild-type plants in an ABA-dependent manner, suggesting that AtSCPL1 is genetically downstream of AtAIRP5/GARU. After drought treatment, the endogenous ABA levels in atscpl1 and atairp5/garu-1 atscpl1-2 mutant leaves were higher than those in wild-type and atairp5/garu leaves. Overall, our results suggest that AtAIRP5/GARU RING E3 Ub ligase functions as a positive regulator of the ABA-mediated drought response by promoting the degradation of AtSCPL1., (© American Society of Plant Biologists 2022. All rights reserved. For permissions, please email: journals.permissions@oup.com.)
- Published
- 2022
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17. Inverse Correlation Between MPSR1 E3 Ubiquitin Ligase and HSP90.1 Balances Cytoplasmic Protein Quality Control.
- Author
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Kim JH, Oh TR, Cho SK, Yang SW, and Kim WT
- Subjects
- Arabidopsis genetics, Arabidopsis Proteins genetics, Gene Expression Regulation, Plant, Gene Silencing, HSP90 Heat-Shock Proteins genetics, MicroRNAs genetics, MicroRNAs metabolism, Protein Folding, Stress, Physiological genetics, Transcriptome genetics, Arabidopsis metabolism, Arabidopsis Proteins metabolism, Cytoplasm metabolism, HSP90 Heat-Shock Proteins metabolism, Ubiquitin-Protein Ligases metabolism
- Abstract
MISFOLDED PROTEIN SENSING RING1 (MPSR1) is a chaperone-independent E3 ubiquitin ligase that participates in protein quality control by eliminating misfolded proteins in Arabidopsis ( Arabidopsis thaliana ). Here, we report that in the early stages of proteotoxic stress, cellular levels of MPSR1 increased immediately, whereas levels of HEAT SHOCK PROTEIN90.1 (AtHSP90.1) were unaltered despite massively upregulated transcription. At this stage, the gene-silencing pathway mediated by microRNA 414 (miR414) suppressed AtHSP90.1 translation. By contrast, under prolonged stress, AtHSP90.1 was not suppressed, and instead competed with MPSR1 to act on misfolded proteins, promoting the destruction of MPSR1. Deficiency or excess of MPSR1 significantly abolished or intensified the suppression of AtHSP90.1, respectively. Similar to the MPSR1 -overexpressing transgenic plants, the miR414 -overexpressing plants showed an increased tolerance to proteotoxic stress as compared to the wild-type plants. Although the functional relationship between MPSR1 and miR414 remains unclear, both MPSR1 and miR414 demonstrated negative modulation of the expression of AtHSP90.1. The inverse correlation between MPSR1 and AtHSP90.1 via miR414 may adjust the set-point of the HSP90-mediated protein quality control process in response to increasing stress intensity in Arabidopsis., (© 2019 American Society of Plant Biologists. All Rights Reserved.)
- Published
- 2019
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18. ROS1-Dependent DNA Demethylation Is Required for ABA-Inducible NIC3 Expression.
- Author
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Kim JS, Lim JY, Shin H, Kim BG, Yoo SD, Kim WT, and Huh JH
- Subjects
- Arabidopsis genetics, Arabidopsis Proteins genetics, Arabidopsis Proteins physiology, DNA Methylation, Epigenomics, Gene Expression Regulation, Plant, Metabolic Networks and Pathways genetics, Nicotinamidase genetics, Nicotinamidase metabolism, Nuclear Proteins genetics, Nuclear Proteins metabolism, Abscisic Acid metabolism, Arabidopsis Proteins metabolism, DNA Demethylation, Nuclear Proteins physiology
- Abstract
DNA methylation plays an important role in diverse developmental processes in many eukaryotes, including the response to environmental stress. Abscisic acid (ABA) is a plant hormone that is up-regulated under stress. The involvement of DNA methylation in the ABA response has been reported but is poorly understood. DNA demethylation is a reverse process of DNA methylation and often induces structural changes of chromatin leading to transcriptional activation. In Arabidopsis ( Arabidopsis thaliana) , active DNA demethylation depends on the activity of REPRESSOR OF SILENCING 1 (ROS1), which directly excises 5-methylcytosine from DNA. Here we showed that ros1 mutants were hypersensitive to ABA during early seedling development and root elongation. Expression levels of some ABA-inducible genes were decreased in ros1 mutants, and more than 60% of their proximal regions became hypermethylated, indicating that a subset of ABA-inducible genes are under the regulation of ROS1-dependent DNA demethylation. Notable among them is NICOTINAMIDASE 3 ( NIC3 ) that encodes an enzyme that converts nicotinamide to nicotinic acid in the NAD
+ salvage pathway. Many enzymes in this pathway are known to be involved in stress responses. The nic3 mutants display hypersensitivity to ABA, whereas overexpression of NIC3 restores normal ABA responses. Our data suggest that NIC3 is responsive to ABA but requires ROS1-mediated DNA demethylation at the promoter as a prerequisite to transcriptional activation. These findings suggest that ROS1-induced active DNA demethylation maintains the active state of NIC3 transcription in response to ABA., (© 2019 American Society of Plant Biologists. All Rights Reserved.)- Published
- 2019
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19. AtAIRP2 E3 Ligase Affects ABA and High-Salinity Responses by Stimulating Its ATP1/SDIRIP1 Substrate Turnover.
- Author
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Oh TR, Kim JH, Cho SK, Ryu MY, Yang SW, and Kim WT
- Subjects
- Arabidopsis drug effects, Arabidopsis genetics, Cell Compartmentation, Cytosol drug effects, Cytosol metabolism, Down-Regulation genetics, Epistasis, Genetic drug effects, Genetic Complementation Test, Germination drug effects, Models, Biological, Molecular Chaperones metabolism, Plant Epidermis cytology, Proteasome Endopeptidase Complex metabolism, Protein Binding drug effects, Protein Subunits metabolism, Seeds drug effects, Seeds growth & development, Subcellular Fractions metabolism, Substrate Specificity drug effects, Nicotiana cytology, Abscisic Acid pharmacology, Arabidopsis metabolism, Arabidopsis Proteins metabolism, Proton-Translocating ATPases metabolism, Salinity
- Abstract
AtAIRP2 is a cytosolic RING-type E3 ubiquitin ligase that positively regulates an abscisic acid (ABA) response in Arabidopsis ( Arabidopsis thaliana ). Yeast two-hybrid screening using AtAIRP2 as bait identified ATP1 (AtAIRP2 Target Protein1) as a substrate of AtAIRP2. ATP1 was found to be identical to SDIRIP1, which was reported recently to be a negative factor in ABA signaling and a target protein of the RING E3 ligase SDIR1. Accordingly, ATP1 was renamed ATP1/SDIRIP1. A specific interaction between AtAIRP2 and ATP1/SDIRIP1 and ubiquitination of ATP1/SDIRIP1 by AtAIRP2 were demonstrated in vitro and in planta. The turnover of ATP1/SDIRIP1 was regulated by AtAIRP2 in cell-free degradation and protoplast cotransfection assays. The ABA-mediated germination assay of 35S : ATP1/SDIRIP1-RNAi/atairp2 double mutant progeny revealed that ATP1/SDIRIP1 acts downstream of AtAIRP2. AtAIRP2 and SDIR1 reciprocally complemented the ABA- and salt-insensitive germination phenotypes of sdir1 and atairp2 mutants, respectively, indicating their combinatory roles in seed germination. Subcellular localization and bimolecular fluorescence complementation experiments in the presence of MG132, a 26S proteasome inhibitor, showed that AtAIRP2 and ATP1/SDIRIP1 were colocalized to the cytosolic spherical body, which lies in close proximity to the nucleus, in tobacco ( Nicotiana benthamiana ) leaf cells. The 26S proteasome subunits RPN12a and RPT1 and the molecular chaperones HSP70 and HSP101 were colocalized to these discrete punctae-like structures. These results raised the possibility that AtAIRP2 and ATP1/SDIRIP1 interact in the cytosolic spherical compartment. Collectively, our data suggest that the down-regulation of ATP1/SDIRIP1 by AtAIRP2 and SDIR1 RING E3 ubiquitin ligases is critical for ABA and high-salinity responses during germination in Arabidopsis., (© 2017 American Society of Plant Biologists. All Rights Reserved.)
- Published
- 2017
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20. Arabidopsis Small Rubber Particle Protein Homolog SRPs Play Dual Roles as Positive Factors for Tissue Growth and Development and in Drought Stress Responses.
- Author
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Kim EY, Park KY, Seo YS, and Kim WT
- Subjects
- Abscisic Acid metabolism, Amino Acid Sequence, Arabidopsis genetics, Arabidopsis growth & development, Arabidopsis ultrastructure, Arabidopsis Proteins genetics, Cell Proliferation, Cell Wall metabolism, Droughts, Gene Expression Regulation, Developmental, Lipid Droplets, Mutation, Plant Growth Regulators metabolism, Plant Leaves genetics, Plant Leaves growth & development, Plant Leaves physiology, Plant Leaves ultrastructure, Plant Roots genetics, Plant Roots growth & development, Plant Roots physiology, Plant Roots ultrastructure, Plants, Genetically Modified, Polymerization, Seedlings genetics, Seedlings growth & development, Seedlings physiology, Seedlings ultrastructure, Seeds genetics, Seeds growth & development, Seeds physiology, Seeds ultrastructure, Stress, Physiological, Nicotiana genetics, Nicotiana growth & development, Nicotiana physiology, Nicotiana ultrastructure, Antigens, Plant genetics, Arabidopsis physiology, Arabidopsis Proteins metabolism, Gene Expression Regulation, Plant, Plant Proteins genetics
- Abstract
Lipid droplets (LDs) act as repositories for fatty acids and sterols, which are used for various cellular processes such as energy production and membrane and hormone synthesis. LD-associated proteins play important roles in seed development and germination, but their functions in postgermination growth are not well understood. Arabidopsis (Arabidopsis thaliana) contains three SRP homologs (SRP1, SRP2, and SRP3) that share sequence identities with small rubber particle proteins of the rubber tree (Hevea brasiliensis). In this report, the possible cellular roles of SRPs in postgermination growth and the drought tolerance response were investigated. Arabidopsis SRPs appeared to be LD-associated proteins and displayed polymerization properties in vivo and in vitro. SRP-overexpressing transgenic Arabidopsis plants (35S:SRP1, 35S:SRP2, and 35S:SRP3) exhibited higher vegetative and reproductive growth and markedly better tolerance to drought stress than wild-type Arabidopsis. In addition, constitutive over-expression of SRPs resulted in increased numbers of large LDs in postgermination seedlings. In contrast, single (srp1, 35S:SRP2-RNAi, and srp3) and triple (35S:SRP2-RNAi/srp1srp3) loss-of-function mutant lines exhibited the opposite phenotypes. Our results suggest that Arabidopsis SRPs play dual roles as positive factors in postgermination growth and the drought stress tolerance response. The possible relationships between LD-associated proteins and the drought stress response are discussed., (© 2016 American Society of Plant Biologists. All Rights Reserved.)
- Published
- 2016
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21. The Arabidopsis RING E3 ubiquitin ligase AtAIRP3/LOG2 participates in positive regulation of high-salt and drought stress responses.
- Author
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Kim JH and Kim WT
- Subjects
- Abscisic Acid metabolism, Abscisic Acid pharmacology, Amino Acids metabolism, Arabidopsis drug effects, Arabidopsis physiology, Arabidopsis Proteins genetics, Cysteine Proteases metabolism, Droughts, Gene Expression Regulation, Plant, Germination genetics, Mutation, Plant Stomata drug effects, Plant Stomata physiology, Plants, Genetically Modified, Promoter Regions, Genetic, Salinity, Ubiquitin-Protein Ligases genetics, Ubiquitination, Arabidopsis Proteins metabolism, Stress, Physiological, Ubiquitin-Protein Ligases metabolism
- Abstract
Really Interesting New Gene (RING) E3 ubiquitin ligases have been implicated in cellular responses to the stress hormone abscisic acid (ABA) as well as to environmental stresses in higher plants. Here, an ABA-insensitive RING protein3 (atairp3) loss-of-function mutant line in Arabidopsis (Arabidopsis thaliana) was isolated due to its hyposensitivity to ABA during its germination stage as compared with wild-type plants. AtAIRP3 contains a single C3HC4-type RING motif, a putative myristoylation site, and a domain associated with RING2 (DAR2) domain. Unexpectedly, AtAIRP3 was identified as LOSS OF GDU2 (LOG2), which was recently shown to participate in an amino acid export system via interaction with GLUTAMINE DUMPER1. Thus, AtAIRP3 was renamed as AtAIRP3/LOG2. Transcript levels of AtAIRP3/LOG2 were up-regulated by drought, high salinity, and ABA, suggesting a role for this factor in abiotic stress responses. The atairp3/log2-2 knockout mutant and 35S:AtAIRP3-RNAi knockdown transgenic plants displayed impaired ABA-mediated seed germination and stomata closure. Cosuppression and complementation studies further supported a positive role for AtAIRP3/LOG2 in ABA responses. Suppression of AtAIRP3/LOG2 resulted in marked hypersensitive phenotypes toward high salinity and water deficit relative to wild-type plants. These results suggest that Arabidopsis RING E3 AtAIRP3/LOG2 is a positive regulator of the ABA-mediated drought and salt stress tolerance mechanism. Using yeast (Saccharomyces cerevisiae) two-hybrid, in vitro, and in vivo immunoprecipitation, cell-free protein degradation, and in vitro ubiquitination assays, RESPONSIVE TO DEHYDRATION21 was identified as a substrate protein of AtAIRP3/LOG2. Collectively, our data suggest that AtAIRP3/LOG2 plays dual functions in ABA-mediated drought stress responses and in an amino acid export pathway in Arabidopsis.
- Published
- 2013
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22. Roles of four Arabidopsis U-box E3 ubiquitin ligases in negative regulation of abscisic acid-mediated drought stress responses.
- Author
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Seo DH, Ryu MY, Jammes F, Hwang JH, Turek M, Kang BG, Kwak JM, and Kim WT
- Subjects
- Abscisic Acid metabolism, Adaptation, Physiological, Amino Acid Sequence, Arabidopsis drug effects, Arabidopsis genetics, Arabidopsis physiology, Arabidopsis Proteins genetics, Calcium metabolism, Chlorophyll analysis, Gene Expression Regulation, Plant, Genes, Plant, Genetic Complementation Test methods, Hydrogen Peroxide pharmacology, Mannitol pharmacology, Molecular Sequence Data, Phenotype, Plant Roots drug effects, Plant Roots metabolism, Plant Roots physiology, Plant Stomata metabolism, Plant Stomata physiology, Plants, Genetically Modified enzymology, Plants, Genetically Modified genetics, Plants, Genetically Modified metabolism, Plants, Genetically Modified physiology, Signal Transduction, Ubiquitin-Protein Ligases genetics, Abscisic Acid pharmacology, Arabidopsis enzymology, Arabidopsis Proteins metabolism, Droughts, Stress, Physiological, Ubiquitin-Protein Ligases metabolism
- Abstract
AtPUB18 and AtPUB19 are homologous U-box E3 ubiquitin ligases in Arabidopsis (Arabidopsis thaliana). AtPUB19 is a negative regulator of abscisic acid (ABA)-mediated drought responses, whereas the role of AtPUB18 in drought responses is unknown. Here, loss-of-function and overexpression tests identified AtPUB18 as a negative regulator in ABA-mediated stomatal closure and water stress responses. The atpub18-2atpub19-3 double mutant line displayed more sensitivity to ABA and enhanced drought tolerance than each single mutant plant; therefore, AtPUB18 and AtPUB19 are agonistic. Stomatal closure of the atpub18-2atpub19-3 mutant was hypersensitive to hydrogen peroxide (H(2)O(2)) but not to calcium, suggesting that AtPUB18 and AtPUB19 exert negative effects on the ABA signaling pathway downstream of H(2)O(2) and upstream of calcium. AtPUB22 and AtPUB23 are other U-box E3 negative regulators of drought responses. Although atpub22atpub23 was more tolerant to drought stress relative to wild-type plants, its ABA-mediated stomatal movements were highly similar to those of wild-type plants. The atpub18-2atpub19-3atpub22atpub23 quadruple mutant exhibited enhanced tolerance to drought stress as compared with each atpub18-2atpub19-3 and atpub22atpub23 double mutant progeny; however, its stomatal behavior was almost identical to the atpub18-2atpub19-3 double mutant in the presence of ABA, H(2)O(2), and calcium. Overexpression of AtPUB18 and AtPUB19 in atpub22atpub23 effectively hindered ABA-dependent stomatal closure, but overexpression of AtPUB22 and AtPUB23 in atpub18-2atpub19-3 did not inhibit ABA-enhanced stomatal closure, highlighting their ABA-independent roles. Overall, these results suggest that AtPUB18 has a linked function with AtPUB19, but is independent from AtPUB22 and AtPUB23, in negative regulation of ABA-mediated drought stress responses.
- Published
- 2012
- Full Text
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23. The Arabidopsis RING E3 ubiquitin ligase AtAIRP2 plays combinatory roles with AtAIRP1 in abscisic acid-mediated drought stress responses.
- Author
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Cho SK, Ryu MY, Seo DH, Kang BG, and Kim WT
- Subjects
- Amino Acid Sequence, Arabidopsis genetics, Arabidopsis physiology, Arabidopsis Proteins genetics, Cytosol enzymology, Cytosol metabolism, Droughts, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Plant, Germination, Molecular Sequence Data, Mutation, Plant Leaves enzymology, Plant Leaves genetics, Plant Leaves physiology, Plant Roots enzymology, Plant Roots genetics, Plant Roots physiology, Promoter Regions, Genetic, Seedlings enzymology, Seedlings genetics, Seedlings physiology, Seeds physiology, Sequence Alignment, Stress, Physiological, Ubiquitin-Protein Ligases genetics, Up-Regulation, Abscisic Acid metabolism, Arabidopsis enzymology, Arabidopsis Proteins metabolism, Ubiquitin-Protein Ligases metabolism
- Abstract
The ubiquitin (Ub)-26S proteasome pathway is implicated in various cellular processes in higher plants. AtAIRP1, a C3H2C3-type RING (for Really Interesting New Gene) E3 Ub ligase, is a positive regulator in the Arabidopsis (Arabidopsis thaliana) abscisic acid (ABA)-dependent drought response. Here, the AtAIRP2 (for Arabidopsis ABA-insensitive RING protein 2) gene was identified and characterized. AtAIRP2 encodes a cytosolic C3HC4-type RING E3 Ub ligase whose expression was markedly induced by ABA and dehydration stress. Thus, AtAIRP2 belongs to a different RING subclass than AtAIRP1 with a limited sequence identity. AtAIRP2-overexpressing transgenic (35S:AtAIRP2-sGFP) and atairp2 loss-of-function mutant plants exhibited hypersensitive and hyposensitive phenotypes, respectively, to ABA in terms of seed germination, root growth, and stomatal movement. 35S:AtAIRP2-sGFP plants were highly tolerant to severe drought stress, and atairp2 alleles were more susceptible to water stress than were wild-type plants. Higher levels of drought-induced hydrogen peroxide production were detected in 35S:AtAIRP2-sGFP as compared with atairp2 plants. ABA-inducible drought-related genes were up-regulated in 35S:AtAIRP2-sGFP and down-regulated in atairp2 progeny. The positive effects of AtAIRP2 on ABA-induced stress genes were dependent on SNF1-related protein kinases, key components of the ABA signaling pathway. Therefore, AtAIRP2 is involved in positive regulation of ABA-dependent drought stress responses. To address the functional relationship between AtAIRP1 and AtAIRP2, FLAG-AtAIRP1 and AtAIRP2-sGFP genes were ectopically expressed in atairp2-2 and atairp1 plants, respectively. Constitutive expression of FLAG-AtAIRP1 and AtAIRP2-sGFP in atairp2-2 and atairp1 plants, respectively, reciprocally rescued the loss-of-function ABA-insensitive phenotypes during germination. Additionally, atairp1/35S:AtAIRP2-sGFP and atairp2-2/35S:FLAG-AtAIRP1 complementation lines were more tolerant to dehydration stress relative to atairp1 and atairp2-2 single knockout plants. Overall, these results suggest that AtAIRP2 plays combinatory roles with AtAIRP1 in Arabidopsis ABA-mediated drought stress responses.
- Published
- 2011
- Full Text
- View/download PDF
24. The Arabidopsis C3H2C3-type RING E3 ubiquitin ligase AtAIRP1 is a positive regulator of an abscisic acid-dependent response to drought stress.
- Author
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Ryu MY, Cho SK, and Kim WT
- Subjects
- Amino Acid Sequence, Arabidopsis genetics, Arabidopsis Proteins chemistry, Arabidopsis Proteins genetics, DNA, Bacterial genetics, Molecular Sequence Data, Mutation, Sequence Homology, Amino Acid, Up-Regulation, Abscisic Acid metabolism, Arabidopsis enzymology, Arabidopsis Proteins physiology, Droughts, Stress, Physiological, Ubiquitin-Protein Ligases metabolism
- Abstract
Ubiquitination is a eukaryotic posttranslational protein modification that is mediated by the cascade of E1, E2, and E3 ubiquitin (Ub) ligases and is involved in regulating numerous cellular functions. In this study, we obtained 100 different Arabidopsis (Arabidopsis thaliana) T-DNA insertion mutant plants in which RING E3 Ub ligase genes were suppressed and monitored their phenotypes in the presence of exogenous abscisic acid (ABA), a plant stress hormone. One of these loss-of-function mutants displayed ABA-insensitive phenotypes at the germination stage and was named atairp1 (for Arabidopsis ABA-insensitive RING protein 1). AtAIRP1 encodes a cytosolic protein containing a single C3H2C3-type RING motif with in vitro E3 Ub ligase activity. AtAIRP1 was significantly induced by ABA and drought stress. In contrast to atairp1 mutant plants, AtAIRP1-overexpressing transgenic plants (35S:AtAIRP1-sGFP) were hypersensitive to exogenous ABA in terms of radicle emergence, cotyledon development, root elongation, and stomatal closure. Ectopic expression of AtAIRP1-sGFP in atairp1 effectively rescued the loss-of-function ABA-insensitive phenotype. Both 35S:AtAIRP1-sGFP and atairp1/35S:AtAIRP1-sGFP plants accumulated higher amounts of hydrogen peroxide in response to exogenous ABA than did wild-type and atairp1 mutant plants. AtAIRP1 overexpressors were markedly tolerant to severe drought stress, as opposed to atairp1, which was highly susceptible. The levels of drought stress-related genes and basic leucine zipper transcription factor genes were up-regulated in the 35S:AtAIRP1-sGFP lines relative to wild-type and atairp1 mutant plants in response to ABA. Overall, these results suggest that AtAIRP1, a C3H2C3-type RING E3 Ub ligase, is a positive regulator in the Arabidopsis ABA-dependent drought response.
- Published
- 2010
- Full Text
- View/download PDF
25. OsKu70 is associated with developmental growth and genome stability in rice.
- Author
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Hong JP, Byun MY, An K, Yang SJ, An G, and Kim WT
- Subjects
- Amino Acid Sequence, DNA Damage drug effects, DNA Damage physiology, DNA Repair, Gene Expression Regulation, Plant physiology, Genome, Plant, Molecular Sequence Data, Mutation, Oryza genetics, Plant Proteins genetics, Plants, Genetically Modified, Pollen cytology, Gene Expression Regulation, Developmental physiology, Oryza growth & development, Oryza metabolism, Plant Proteins metabolism
- Abstract
The cellular functions of Ku70 in repair of DNA double-stranded breaks and telomere regulation have been described in a wide range of organisms. In this study, we identified the rice (Oryza sativa) Ku70 homolog (OsKu70) from the rice genome database. OsKu70 transcript was detected constitutively in every tissue and developmental stage examined and also in undifferentiated callus cells in rice. Yeast two-hybrid and in vitro pull-down experiments revealed that OsKu70 physically interacts with OsKu80. We obtained loss-of-function osku70 T-DNA knockout mutant lines and constructed transgenic rice plants that overexpress the OsKu70 gene in the sense (35S:OsKu70) or antisense (35S:anti-OsKu70) orientation. The homozygous G2 osku70 mutant lines were more sensitive than wild-type plants to a DNA-damaging agent (0.01%-0.05% methyl-methane sulfonate), consistent with the notion that OsKu70 participates in the DNA repair mechanism. Terminal restriction fragment analysis revealed that telomeres in homozygous G2 osku70 mutants were markedly longer (10-20 kb) than those in wild-type plants (5-10 kb), whereas telomere length in heterozygous G2 osku70 mutant and T2 OsKu70-overexpressing transgenic (35S:OsKu70) rice resembled that of the wild-type plant. In contrast to what was observed in Arabidopsis (Arabidopsis thaliana) atku70 mutants, homozygous G2 osku70 rice plants displayed severe developmental defects in both vegetative and reproductive organs under normal growth conditions, resulting in sterile flowers. Analysis of meiotic progression in pollen mother cells demonstrated that up to 11.1% (seven of 63) of G2 mutant anaphase cells displayed one or more chromosomal fusions. These results suggest that OsKu70 is required for the maintenance of chromosome stability and normal developmental growth in rice plants.
- Published
- 2010
- Full Text
- View/download PDF
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