3 results on '"Ksienzyk B"'
Search Results
2. A clinically applicable gene expression-based score predicts resistance to induction treatment in acute myeloid leukemia.
- Author
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Moser C, Jurinovic V, Sagebiel-Kohler S, Ksienzyk B, Batcha AMN, Dufour A, Schneider S, Rothenberg-Thurley M, Sauerland CM, Görlich D, Berdel WE, Krug U, Mansmann U, Hiddemann W, Braess J, Spiekermann K, Greif PA, Vosberg S, Metzeler KH, Kumbrink J, and Herold T
- Subjects
- Cohort Studies, Cytogenetics, Gene Expression, Humans, Prognosis, Leukemia, Myeloid, Acute diagnosis, Leukemia, Myeloid, Acute drug therapy, Leukemia, Myeloid, Acute genetics
- Abstract
Prediction of resistant disease at initial diagnosis of acute myeloid leukemia (AML) can be achieved with high accuracy using cytogenetic data and 29 gene expression markers (Predictive Score 29 Medical Research Council; PS29MRC). Our aim was to establish PS29MRC as a clinically usable assay by using the widely implemented NanoString platform and further validate the classifier in a more recently treated patient cohort. Analyses were performed on 351 patients with newly diagnosed AML intensively treated within the German AML Cooperative Group registry. As a continuous variable, PS29MRC performed best in predicting induction failure in comparison with previously published risk models. The classifier was strongly associated with overall survival. We were able to establish a previously defined cutoff that allows classifier dichotomization (PS29MRCdic). PS29MRCdic significantly identified induction failure with 59% sensitivity, 77% specificity, and 72% overall accuracy (odds ratio, 4.81; P = 4.15 × 10-10). PS29MRCdic was able to improve the European Leukemia Network 2017 (ELN-2017) risk classification within every category. The median overall survival with high PS29MRCdic was 1.8 years compared with 4.3 years for low-risk patients. In multivariate analysis including ELN-2017 and clinical and genetic markers, only age and PS29MRCdic were independent predictors of refractory disease. In patients aged ≥60 years, only PS29MRCdic remained as a significant variable. In summary, we confirmed PS29MRC as a valuable classifier to identify high-risk patients with AML. Risk classification can still be refined beyond ELN-2017, and predictive classifiers might facilitate clinical trials focusing on these high-risk patients with AML., (© 2021 by The American Society of Hematology. Licensed under Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0), permitting only noncommercial, nonderivative use with attribution. All other rights reserved.)
- Published
- 2021
- Full Text
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3. Genetic heterogeneity of cytogenetically normal AML with mutations of CEBPA .
- Author
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Konstandin NP, Pastore F, Herold T, Dufour A, Rothenberg-Thurley M, Hinrichsen T, Ksienzyk B, Tschuri S, Schneider S, Hoster E, Berdel WE, Woermann BJ, Sauerland MC, Braess J, Bohlander SK, Klein HG, Hiddemann W, Metzeler KH, and Spiekermann K
- Subjects
- Adolescent, Adult, Aged, CCAAT-Enhancer-Binding Proteins metabolism, Female, Humans, Male, Middle Aged, Mutation, Prognosis, Young Adult, CCAAT-Enhancer-Binding Protein-alpha genetics, CCAAT-Enhancer-Binding Proteins genetics, Cytogenetics methods, Genetic Heterogeneity drug effects
- Abstract
Biallelic mutations of the CCAAT/enhancer binding protein α ( CEBPA ) gene define a distinct genetic entity of acute myeloid leukemia (AML) with favorable prognosis. The presence of GATA2 and CSF3R mutations that are specifically associated with this subgroup but not mutated in all samples suggests a genetic heterogeneity of bi CEBPA -mutated AML. We characterized the mutational landscape of CEBPA -mutated cytogenetically normal AML by targeted amplicon resequencing. We analyzed 48 biallelically mutated CEBPA (bi CEBPA ), 32 monoallelically mutated CEBPA (mo CEBPA ), and 287 wild-type CEBPA (wt CEBPA ) patient samples from German AML Cooperative Group studies or registry. Targeted sequencing of 42 genes revealed that mo CEBPA patients had significantly more additional mutations and additional mutated genes than bi CEBPA patients. Within the group of bi CEBPA patients, we identified 2 genetic subgroups defined by the presence or absence of mutations in chromatin/DNA modifiers (C), cohesin complex (C), and splicing (S) genes: bi CEBPA
CCSpos (25/48 [52%]) and bi CEBPACCSneg (23/48 [48%]). Equivalent subgroups were identified in 51 bi CEBPA patients from the Cancer Genome Project. Patients in the bi CEBPACCSpos group were significantly older and had poorer overall survival and lower complete remission rates following intensive chemotherapy regimens compared with patients in the bi CEBPACCSneg group. Patients with available remission samples from the bi CEBPACCSpos group cleared the bi CEBPA mutations, but most had persisting CCS mutations in complete remission, suggesting the presence of a preleukemic clone. In conclusion, CCS mutations define a distinct biological subgroup of bi CEBPA AML that might refine prognostic classification of AML. This trial was registered at www.clinicaltrials.gov as #NCT00266136 and NCT01382147., (© 2018 by The American Society of Hematology.)- Published
- 2018
- Full Text
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