1. Human blood monocytes and platelets share a cell surface component
- Author
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J J, Burckhardt, W H, Anderson, J F, Kearney, and M D, Cooper
- Subjects
Blood Platelets ,Mice, Inbred BALB C ,Immunology ,Antibodies, Monoclonal ,Haplorhini ,Cell Biology ,Hematology ,Biochemistry ,Monocytes ,Molecular Weight ,Mice ,Leukemia, Myeloid ,Pronase ,Antigens, Surface ,Animals ,Humans - Abstract
We describe a surface determinant shared by human monocytes and cells of the megakaryocytic axis that has been identified using a mouse monoclonal antibody. This monocyte-platelet antigen (MPA) is expressed on all (greater than 99%) of peripheral blood monocytes, platelets, and megakaryocytes. It is also expressed weakly on the monocytic cell line U937 and the promyelocytic line HL60 and is present on cells from 3 of 4 AML patients examined. It is absent from polymorphonuclear leukocytes, T and B lymphocytes, erythrocytes, and a panel of hematopoietic cell lines. MPA is stripped from monocyte membranes with pronase and is reexpressed overnight. The determinant is carried on a noncovalently linked biomolecular complex with molecular weights of 93,000 and 135,000.
- Published
- 1982
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