1. HLA DR associated invariant chain is highly expressed in chronic lymphocytic leukemia
- Author
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Franco NARNI, Kudo, J., Mars, W., Calabretta, B., Florine, D. L., Barlogie, B., and Saunders, G. F.
- Subjects
Paper ,chronic lymphocuytic leukemia ,HLA ,invariant chain ,Immunology ,Histocompatibility Antigens Class II ,Collodion ,Genetic Variation ,Nucleic Acid Hybridization ,HLA-DR Antigens ,Cell Biology ,Hematology ,Biochemistry ,Clone Cells ,Leukemia, Lymphoid ,hemic and lymphatic diseases ,Humans ,Electrophoresis, Polyacrylamide Gel ,RNA, Messenger ,Filtration - Abstract
Total RNA extracted from peripheral blood lymphocytes of a patient with B-cell chronic lymphocytic leukemia (CLL) and the poly (A+) RNA was purified. A cDNA library was constructed and approximately 4,000 clones were screened in order to identify genes preferentially expressed in CLL. A relatively low repetition frequency characterizes the majority of the abundant mRNA species present in CLL lymphocytes. One clone, corresponding to the mRNA encoding the HLA-DR-associated invariant chain, was selected and its expression was examined in different leukemic cell populations and in normal tissues. DNA-RNA hybridization studies showed that the invariant chain mRNA (In-mRNA) is detectable in RNA preparations from human blood cells and their precursors, whereas no In-mRNA is found in several other tissues examined. Among various normal and leukemic leukocyte populations, the highest levels of In- mRNA are found in CLL. Therefore, a role of In-chain mRNA as a marker of CLL is proposed. Our data support a relationship between high levels of invariant chain mRNA and the out of cycle condition of CLL peripheral blood lymphocytes.
- Published
- 1986