1. Cell Cycle Characteristics of Crenarchaeota: Unity among Diversity▿
- Author
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Laurence Malandrin, Magnus Lundgren, Stefan Eriksson, Rolf Bernander, Harald Huber, Uppsala University, UMR1300 Bio-agression, Epidémiologie et Analyse de Risque, Institut National de la Recherche Agronomique (INRA)-ENVN, and University of Regensburg
- Subjects
DNA Replication ,Time Factors ,Cell division ,PYROBACULUM-AEROPHILUM ,DNA Replication Timing ,Sulfolobus tokodaii ,Microbiology ,Microbial Cell Biology ,03 medical and health sciences ,INITIATION ,Crenarchaeota ,DNA-CONTENT ,Chromosome Segregation ,NUCLEOID STRUCTURE ,Aeropyrum pernix ,Microscopy, Phase-Contrast ,Molecular Biology ,030304 developmental biology ,Genetics ,0303 health sciences ,biology ,030306 microbiology ,Pyrobaculum ,Cell Cycle ,GENOME SEQUENCE ,biology.organism_classification ,Flow Cytometry ,GENUS SULFOLOBUS ,CHROMOSOME-REPLICATION ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,Microscopy, Fluorescence ,THERMOPHILIC ARCHAEA ,ORIGINS ,SP-NOV ,Archaea ,Acidianus - Abstract
The hyperthermophilic archaea Acidianus hospitalis , Aeropyrum pernix , Pyrobaculum aerophilum , Pyrobaculum calidifontis , and Sulfolobus tokodaii representing three different orders in the phylum Crenarchaeota were analyzed by flow cytometry and combined phase-contrast and epifluorescence microscopy. The overall organization of the cell cycle was found to be similar in all species, with a short prereplicative period and a dominant postreplicative period that accounted for 64 to 77% of the generation time. Thus, in all Crenarchaeota analyzed to date, cell division and initiation of chromosome replication occur in close succession, and a long time interval separates termination of replication from cell division. In Pyrobaculum , chromosome segregation overlapped with or closely followed DNA replication, and further genome separation appeared to occur concomitant with cellular growth. Cell division in P. aerophilum took place without visible constriction.
- Published
- 2008