1. Molecular Detection of Viable Bacterial Pathogens in Water by Ratiometric Pre-rRNA Analysis ▿ †
- Author
-
Kris M. Weigel, Clarita Lefthand-Begay, Gerard A. Cangelosi, and John Scott Meschke
- Subjects
DNA, Bacterial ,Molecular Sequence Data ,Colony Count, Microbial ,Biology ,Applied Microbiology and Biotechnology ,complex mixtures ,DNA, Ribosomal ,Polymerase Chain Reaction ,Microbiology ,law.invention ,Bacterial genetics ,chemistry.chemical_compound ,law ,RNA, Ribosomal, 16S ,Methods ,RNA Precursors ,Cloning, Molecular ,Gene ,Polymerase chain reaction ,Cloning ,Microbial Viability ,Ecology ,Bacteria ,Reverse Transcriptase Polymerase Chain Reaction ,Biodiversity ,Ribosomal RNA ,biology.organism_classification ,Culture Media ,enzymes and coenzymes (carbohydrates) ,RNA, Bacterial ,chemistry ,Genes, Bacterial ,RNA, Ribosomal ,Water Microbiology ,DNA ,Food Science ,Biotechnology - Abstract
Ratiometric pre-rRNA analysis (RPA) detects the replenishment of rRNA precursors that occurs rapidly upon nutritional stimulation of bacterial cells. In contrast to DNA detection by PCR, RPA distinguishes viable from inactivated bacteria. It exhibits promise as a molecular viability test for pathogens in water and other environmental samples.
- Published
- 2009