Search

Your search keyword '"Yen, Hui-Ling"' showing total 20 results
Start Over Author "Yen, Hui-Ling" Publisher american society for microbiology
20 results on '"Yen, Hui-Ling"'

1. Imprinted Anti-Hemagglutinin and Anti-Neuraminidase Antibody Responses after Childhood Infections of A(H1N1) and A(H1N1)pdm09 Influenza Viruses

Author

Daulagala, Pavithra, primary, Mann, Brian R., additional, Leung, Kathy, additional, Lau, Eric H. Y., additional, Yung, Louise, additional, Lei, Ruipeng, additional, Nizami, Sarea I. N., additional, Wu, Joseph T., additional, Chiu, Susan S., additional, Daniels, Rodney S., additional, Wu, Nicholas C., additional, Wentworth, David, additional, Peiris, Malik, additional, and Yen, Hui-Ling, additional

Published
2023
Full Text
View/download PDF

2. Neutralizing Monoclonal Antibodies That Target the Spike Receptor Binding Domain Confer Fc Receptor-Independent Protection against SARS-CoV-2 Infection in Syrian Hamsters

Author

Su, Wen, primary, Sia, Sin Fun, additional, Schmitz, Aaron J., additional, Bricker, Traci L., additional, Starr, Tyler N., additional, Greaney, Allison J., additional, Turner, Jackson S., additional, Mohammed, Bassem M., additional, Liu, Zhuoming, additional, Choy, Ka Tim, additional, Darling, Tamarand L., additional, Joshi, Astha, additional, Cheng, Ka Man, additional, Wong, Alvina Y. L., additional, Harastani, Houda H., additional, Nicholls, John M., additional, Whelan, Sean P. J., additional, Bloom, Jesse D., additional, Yen, Hui-Ling, additional, Ellebedy, Ali H., additional, and Boon, Adrianus C. M., additional

Published
2021
Full Text
View/download PDF

3. Phenotypic and Functional Characteristics of a Novel Influenza Virus Hemagglutinin-Specific Memory NK Cell

Author

Zheng, Jian, primary, Wen, Liyan, additional, Yen, Hui-Ling, additional, Liu, Ming, additional, Liu, Yinping, additional, Teng, Ooiean, additional, Wu, Wing-Fung, additional, Ni, Ke, additional, Lam, Kowk-Tai, additional, Huang, Chunyu, additional, Yang, Jiashuang, additional, Lau, Yu-Lung, additional, Perlman, Stanley, additional, Peiris, Malik, additional, and Tu, Wenwei, additional

Published
2021
Full Text
View/download PDF

4. Evaluation of a SARS-CoV-2 Surrogate Virus Neutralization Test for Detection of Antibody in Human, Canine, Cat, and Hamster Sera

Author

Perera, Ranawaka A. P. M., primary, Ko, Ronald, additional, Tsang, Owen T. Y., additional, Hui, David S. C., additional, Kwan, Mike Y. M., additional, Brackman, Christopher J., additional, To, Esther M. W., additional, Yen, Hui-ling, additional, Leung, Kathy, additional, Cheng, Samuel M. S., additional, Chan, Kin Ho, additional, Chan, Karl C. K., additional, Li, Ka-Chi, additional, Saif, Linda, additional, Barrs, Vanessa R., additional, Wu, Joseph T., additional, Sit, Thomas H. C., additional, Poon, Leo L. M., additional, and Peiris, Malik, additional

Published
2021
Full Text
View/download PDF

5. CLEC5A-Mediated Enhancement of the Inflammatory Response in Myeloid Cells Contributes to Influenza Virus Pathogenicity In Vivo

Author

Teng, Ooiean, primary, Chen, Szu-Ting, additional, Hsu, Tsui-Ling, additional, Sia, Sin Fun, additional, Cole, Suzanne, additional, Valkenburg, Sophie A., additional, Hsu, Tzu-Yun, additional, Zheng, Jian Teddy, additional, Tu, Wenwei, additional, Bruzzone, Roberto, additional, Peiris, Joseph Sriyal Malik, additional, Hsieh, Shie-Liang, additional, and Yen, Hui-Ling, additional

Published
2017
Full Text
View/download PDF

6. Transmission of H7N9 Influenza Viruses with a Polymorphism at PB2 Residue 627 in Chickens and Ferrets

Author

Luk, Geraldine S. M., primary, Leung, Connie Y. H., additional, Sia, Sin Fun, additional, Choy, Ka-Tim, additional, Zhou, Jie, additional, Ho, Candy C. K., additional, Cheung, Peter P. H., additional, Lee, Elaine F., additional, Wai, Chris K. L., additional, Li, Pamela C. H., additional, Ip, Sin-Ming, additional, Poon, Leo L. M., additional, Lindsley, William G., additional, Peiris, Malik, additional, and Yen, Hui-Ling, additional

Published
2015
Full Text
View/download PDF

8. Tissue Tropism of Swine Influenza Viruses and Reassortants in Ex Vivo Cultures of the Human Respiratory Tract and Conjunctiva

Author

Chan, Renee W. Y., primary, Kang, Sara S. R., additional, Yen, Hui-Ling, additional, Li, Alan C. L., additional, Tang, Lynsia L. S., additional, Yu, Wendy C. L., additional, Yuen, Kit M., additional, Chan, Icarus W. W., additional, Wong, Diana D. Y., additional, Lai, Wico W., additional, Kwong, Dora L. W., additional, Sihoe, Alan D. L., additional, Poon, Leo L. M., additional, Guan, Yi, additional, Nicholls, John M., additional, Peiris, J. S. Malik, additional, and Chan, Michael C. W., additional

Published
2011
Full Text
View/download PDF

14. Inefficient Transmission of H5N1 Influenza Viruses in a Ferret Contact Model

Author

Yen, Hui-Ling, primary, Lipatov, Aleksandr S., additional, Ilyushina, Natalia A., additional, Govorkova, Elena A., additional, Franks, John, additional, Yilmaz, Neziha, additional, Douglas, Alan, additional, Hay, Alan, additional, Krauss, Scott, additional, Rehg, Jerold E., additional, Hoffmann, Erich, additional, and Webster, Robert G., additional

Published
2007
Full Text
View/download PDF

16. Lethality to Ferrets of H5N1 Influenza Viruses Isolated from Humans and Poultry in 2004

Author

Govorkova, Elena A., primary, Rehg, Jerold E., additional, Krauss, Scott, additional, Yen, Hui-Ling, additional, Guan, Yi, additional, Peiris, Malik, additional, Nguyen, Tien D., additional, Hanh, Thi H., additional, Puthavathana, Pilaipanl, additional, Long, Hoang T., additional, Buranathai, Chantanee, additional, Lim, Wilina, additional, Webster, Robert G., additional, and Hoffman, Erich, additional

Published
2006
Full Text
View/download PDF

18. Lethality to Ferrets of H5N1 Influenza Viruses Isolated from Humans and Poultry in 2004

Author

Govorkova, Elena A., primary, Rehg, Jerold E., additional, Krauss, Scott, additional, Yen, Hui-Ling, additional, Guan, Yi, additional, Peiris, Malik, additional, Nguyen, Tien D., additional, Hanh, Thi H., additional, Puthavathana, Pilipan, additional, Long, Hoang T., additional, Buranathai, Chantanee, additional, Lim, Wilina, additional, Webster, Robert G., additional, and Hoffmann, Erich, additional

Published
2005
Full Text
View/download PDF

19. Robustness of the Ferret Model for Influenza Risk Assessment Studies: a Cross-Laboratory Exercise.

Author

Belser JA, Lau EHY, Barclay W, Barr IG, Chen H, Fouchier RAM, Hatta M, Herfst S, Kawaoka Y, Lakdawala SS, Lee LYY, Neumann G, Peiris M, Perez DR, Russell C, Subbarao K, Sutton TC, Webby RJ, Yang H, and Yen HL

Subjects
Animals, Ferrets, Humans, Laboratories, Lung, Risk Assessment, Influenza A Virus, H1N1 Subtype, Influenza, Human, Orthomyxoviridae Infections
Abstract

Past pandemic influenza viruses with sustained human-to-human transmissibility have emerged from animal influenza viruses. Employment of experimental models to assess the pandemic risk of emerging zoonotic influenza viruses provides critical information supporting public health efforts. Ferret transmission experiments have been utilized to predict the human-to-human transmission potential of novel influenza viruses. However, small sample sizes and a lack of standardized protocols can introduce interlaboratory variability, complicating interpretation of transmission experimental data. To assess the range of variation in ferret transmission experiments, a global exercise was conducted by 11 laboratories using two common stock H1N1 influenza viruses with different transmission characteristics in ferrets. Parameters known to affect transmission were standardized, including the inoculation route, dose, and volume, as well as a strict 1:1 donor/contact ratio for respiratory droplet transmission. Additional host and environmental parameters likely to affect influenza transmission kinetics were monitored and analyzed. The overall transmission outcomes for both viruses across 11 laboratories were concordant, suggesting the robustness of the ferret model for zoonotic influenza risk assessment. Among environmental parameters that varied across laboratories, donor-to-contact airflow directionality was associated with increased transmissibility. To attain high confidence in identifying viruses with moderate to high transmissibility or low transmissibility under a smaller number of participating laboratories, our analyses support the notion that as few as three but as many as five laboratories, respectively, would need to independently perform viral transmission experiments with concordant results. This exercise facilitates the development of a more homogenous protocol for ferret transmission experiments that are employed for the purposes of risk assessment. IMPORTANCE Following detection of a novel virus, rapid characterization efforts (both in vitro and in vivo ) are undertaken at numerous laboratories worldwide to evaluate the relative risk posed to human health. Aggregation of these data are critical, but the use of nonstandardized protocols can make interpretation of divergent results a challenge. For evaluation of virus transmissibility, a multifactorial trait which can only be evaluated in vivo , identifying intrinsic levels of variability between groups can improve the utility of these data, as well as ensure that experiments are performed with sufficient replication to ensure high confidence in compiled results. Using the ferret transmission model and two influenza A viruses, we conducted a multicenter standardization exercise to improve the interpretation of transmission data generated during risk assessment activities; this exercise serves as a model for future efforts employing both in vitro and in vivo models against possible pandemic pathogens.

Published
2022
Full Text
View/download PDF

20. CLEC5A-Mediated Enhancement of the Inflammatory Response in Myeloid Cells Contributes to Influenza Virus Pathogenicity In Vivo.

Author

Teng O, Chen ST, Hsu TL, Sia SF, Cole S, Valkenburg SA, Hsu TY, Zheng JT, Tu W, Bruzzone R, Peiris JSM, Hsieh SL, and Yen HL

Subjects
Animals, Antibodies pharmacology, Chemokine CXCL10 genetics, Chemokine CXCL10 immunology, Gene Expression Regulation, Hemagglutinin Glycoproteins, Influenza Virus genetics, Host-Pathogen Interactions, Humans, Influenza A Virus, H1N1 Subtype growth & development, Influenza A Virus, H1N1 Subtype immunology, Influenza A Virus, H5N1 Subtype growth & development, Influenza A Virus, H5N1 Subtype immunology, Interferon-alpha genetics, Interferon-alpha immunology, Lectins, C-Type antagonists & inhibitors, Lectins, C-Type genetics, Lentivirus genetics, Lentivirus immunology, Lung drug effects, Lung immunology, Lung virology, Macrophages drug effects, Macrophages immunology, Macrophages virology, Macrophages, Alveolar drug effects, Macrophages, Alveolar immunology, Macrophages, Alveolar virology, Mice, Mice, Inbred C57BL, Mice, Knockout, Orthomyxoviridae Infections genetics, Orthomyxoviridae Infections mortality, Orthomyxoviridae Infections virology, Primary Cell Culture, Protein Binding, Protein Isoforms genetics, Protein Isoforms immunology, RNA, Small Interfering genetics, RNA, Small Interfering metabolism, Receptors, Cell Surface antagonists & inhibitors, Receptors, Cell Surface genetics, Survival Analysis, Toll-Like Receptor 3 genetics, Toll-Like Receptor 3 immunology, Tumor Necrosis Factor-alpha genetics, Tumor Necrosis Factor-alpha immunology, Hemagglutinin Glycoproteins, Influenza Virus immunology, Influenza A Virus, H1N1 Subtype pathogenicity, Influenza A Virus, H5N1 Subtype pathogenicity, Lectins, C-Type immunology, Orthomyxoviridae Infections immunology, Receptors, Cell Surface immunology
Abstract

Human infections with influenza viruses exhibit mild to severe clinical outcomes as a result of complex virus-host interactions. Induction of inflammatory mediators via pattern recognition receptors may dictate subsequent host responses for pathogen clearance and tissue damage. We identified that human C-type lectin domain family 5 member A (CLEC5A) interacts with the hemagglutinin protein of influenza viruses expressed on lentiviral pseudoparticles through lectin screening. Silencing CLEC5A gene expression, blocking influenza-CLEC5A interactions with anti-CLEC5A antibodies, or dampening CLEC5A-mediated signaling using a spleen tyrosine kinase inhibitor consistently reduced the levels of proinflammatory cytokines produced by human macrophages without affecting the replication of influenza A viruses of different subtypes. Infection of bone marrow-derived macrophages from CLEC5A-deficient mice showed reduced levels of tumor necrosis factor alpha (TNF-α) and IP-10 but elevated alpha interferon (IFN-α) compared to those of wild-type mice. The heightened type I IFN response in the macrophages of CLEC5A-deficient mice was associated with upregulated TLR3 mRNA after treatment with double-stranded RNA. Upon lethal challenges with a recombinant H5N1 virus, CLEC5A-deficient mice showed reduced levels of proinflammatory cytokines, decreased immune cell infiltration in the lungs, and improved survival compared to the wild-type mice, despite comparable viral loads noted throughout the course of infection. The survival difference was more prominent at a lower dose of inoculum. Our results suggest that CLEC5A-mediated enhancement of the inflammatory response in myeloid cells contributes to influenza pathogenicity in vivo and may be considered a therapeutic target in combination with effective antivirals. Well-orchestrated host responses together with effective viral clearance are critical for optimal clinical outcome after influenza infections., Importance: Multiple pattern recognition receptors work in synergy to sense viral RNA or proteins synthesized during influenza replication and mediate host responses for viral control. Well-orchestrated host responses may help to maintain the inflammatory response to minimize tissue damage while inducing an effective adaptive immune response for viral clearance. We identified that CLEC5A, a C-type lectin receptor which has previously been reported to mediate flavivirus-induced inflammatory responses, enhanced induction of proinflammatory cytokines and chemokines in myeloid cells after influenza infections. CLEC5A-deficient mice infected with influenza virus showed reduced inflammation in the lungs and improved survival compared to that of the wild-type mice despite comparable viral loads. The survival difference was more prominent at a lower dose of inoculum. Collectively, our results suggest that dampening CLEC5A-mediated inflammatory responses in myeloid cells reduces immunopathogenesis after influenza infections., (Copyright © 2016 Teng et al.)

Published
2016
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results