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Your search keyword '"Spitzer, E"' showing total 17 results
Start Over Author "Spitzer, E" Publisher american society for microbiology
17 results on '"Spitzer, E"'

2. Detection and Serotyping of Dengue Virus in Serum Samples by Multiplex Reverse Transcriptase PCR-Ligase Detection Reaction Assay

Author

Das, S., primary, Pingle, M. R., additional, Muñoz-Jordán, J., additional, Rundell, M. S., additional, Rondini, S., additional, Granger, K., additional, Chang, G.-J. J., additional, Kelly, E., additional, Spier, E. G., additional, Larone, D., additional, Spitzer, E., additional, Barany, F., additional, and Golightly, L. M., additional

Published
2008
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3. Development of Multiplex PCR-Ligase Detection Reaction Assay for Detection of West Nile Virus

Author

Rondini, S., primary, Pingle, M. R., additional, Das, S., additional, Tesh, R., additional, Rundell, M. S., additional, Hom, J., additional, Stramer, S., additional, Turner, K., additional, Rossmann, S. N., additional, Lanciotti, R., additional, Spier, E. G., additional, Muñoz-Jordán, J., additional, Larone, D., additional, Spitzer, E., additional, Barany, F., additional, and Golightly, L. M., additional

Published
2008
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16. Multiplexed identification of blood-borne bacterial pathogens by use of a novel 16S rRNA gene PCR-ligase detection reaction-capillary electrophoresis assay.

Author

Pingle MR, Granger K, Feinberg P, Shatsky R, Sterling B, Rundell M, Spitzer E, Larone D, Golightly L, and Barany F

Subjects
Bacteria genetics, Bacteria isolation & purification, Bioterrorism, Blood-Borne Pathogens isolation & purification, Genes, rRNA, Humans, Ligase Chain Reaction, Polymerase Chain Reaction methods, Sensitivity and Specificity, Bacteria classification, Blood-Borne Pathogens classification, Electrophoresis, Capillary methods, RNA, Ribosomal, 16S genetics
Abstract

We have developed a novel high-throughput PCR-ligase detection reaction-capillary electrophoresis (PCR-LDR-CE) assay for the multiplexed identification of 20 blood-borne pathogens (Staphylococcus epidermidis, Staphylococcus aureus, Bacillus cereus, Enterococcus faecalis, Enterococcus faecium, Listeria monocytogenes, Streptococcus pneumoniae, Streptococcus pyogenes, Streptococcus agalactiae, Escherichia coli, Klebsiella pneumoniae, Haemophilus influenzae, Pseudomonas aeruginosa, Acinetobacter baumannii, Neisseria meningitidis, Bacteroides fragilis, Bacillus anthracis, Yersinia pestis, Francisella tularensis, and Brucella abortus), the last four of which are biothreat agents. The method relies on the amplification of two regions within the bacterial 16S rRNA gene, using universal PCR primers and querying the identity of specific single-nucleotide polymorphisms within the amplified regions in a subsequent LDR. The ligation products vary in color and size and are separated by CE. Each organism generates a specific pattern of ligation products, which can be used to distinguish the pathogens using an automated software program we developed for that purpose. The assay has been verified on 315 clinical isolates and demonstrated a detection sensitivity of 98%. Additionally, 484 seeded blood cultures were tested, with a detection sensitivity of 97.7%. The ability to identify geographically variant strains of the organisms was determined by testing 132 isolates obtained from across the United States. In summary, the PCR-LDR-CE assay can successfully identify, in a multiplexed fashion, a panel of 20 blood-borne pathogens with high sensitivity and specificity.

Published
2007
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17. Prevalence in Cryptococcus neoformans strains of a polysaccharide epitope which can elicit protective antibodies.

Author

Cleare W, Mukherjee S, Spitzer ED, and Casadevall A

Subjects
Agglutination, Animals, Antibodies, Monoclonal immunology, Cell Line immunology, Cryptococcus neoformans classification, Epitopes immunology, Fluorescent Antibody Technique, Indirect, Macrophages immunology, Mice, Phagocytosis immunology, Polysaccharides immunology, Serotyping, Antibodies, Fungal immunology, Cryptococcus neoformans immunology
Abstract

Monoclonal antibody (MAb) 2H1 binds to an epitope in the capsule of Cryptococcus neoformans that can elicit protective antibodies. The binding of MAb 2H1 to C. neoformans strains was studied by agglutination, immunofluorescence, and phagocytosis assays. The MAb 2H1 epitope was present in all 21 isolates studied, including those recovered from patients with recurrent infections.

Published
1994
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