1. Evaluation of Two New Membrane-Based and Microtiter Plate Enzyme-Linked Immunosorbent Assays for Detection of Campylobacter jejuni in Stools of Bangladeshi Children.
- Author
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Schnee AE, Haque R, Taniuchi M, Uddin MJ, and Petri WA Jr
- Subjects
- Antigens, Bacterial analysis, Bangladesh, Campylobacter coli immunology, Campylobacter jejuni immunology, Child, Preschool, Diagnostic Tests, Routine, Diarrhea diagnosis, Humans, Infant, Infant, Newborn, Polymerase Chain Reaction standards, Sensitivity and Specificity, Bacteriological Techniques methods, Campylobacter Infections diagnosis, Campylobacter coli isolation & purification, Campylobacter jejuni isolation & purification, Enzyme-Linked Immunosorbent Assay, Feces microbiology
- Abstract
Two new monoclonal antibody-based, sandwich enzyme immunoassays (EIAs) for fecal antigen detection of Campylobacter jejuni or Campylobacter coli were evaluated using diarrheal stool specimens from a cohort of children in Bangladesh. These children routinely harbor multiple enteric pathogens, often at levels that make it difficult to assign diarrheal symptoms to a causative agent. A panel of 158 PCR-positive specimens with a broad range of C. jejuni / C. coli DNA cycle threshold ( C
T ) values was used to assess the ability of the two tests to detect C. jejuni / C. coli antigen amounts that varied widely. A panel of 100 C. jejuni / C. coli PCR-negative specimens was used to verify that the assays correctly identified specimens as negative when the sample contained other enteric pathogens. Further analysis was conducted on a subset of 46 specimens that contained particularly substantial amounts of C. jejuni / C. coli ( CT of ≤19.7) that previous studies have ascribed as "diarrhea-associated." The Quik Chek rapid EIA and the Chek enzyme-linked immunosorbent assays (ELISAs) had a sensitivity of 95.7% for these specimens (specificities, 97% and 96%, respectively), supporting the usefulness of the new Chek and Quik Chek assays in symptomatic presentations, where Campylobacter is the likely etiology., (Copyright © 2018 Schnee et al.)- Published
- 2018
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