1. The Isolation of Orientia tsutsugamushi and Rickettsia typhi from Human Blood through Mammalian Cell Culture: a Descriptive Series of 3,227 Samples and Outcomes in the Lao People’s Democratic Republic
- Author
-
Davanh Sengdatka, Tamalee Roberts, Paul N. Newton, Matthew T. Robinson, Vanheuang Phommadeechack, Damien K. Ming, Weerawat Phuklia, Vilada Chansamouth, Stuart D. Blacksell, and Phonepasith Panyanivong
- Subjects
0301 basic medicine ,Microbiology (medical) ,clinical methods ,Orientia tsutsugamushi ,Isolation (health care) ,Chlamydiology and Rickettsiology ,030231 tropical medicine ,030106 microbiology ,Cell Culture Techniques ,Murine typhus ,Microbiology ,Serology ,03 medical and health sciences ,Mice ,0302 clinical medicine ,07 Agricultural and Veterinary Sciences ,Rickettsia typhi ,Medicine ,Animals ,Humans ,Rickettsia ,11 Medical and Health Sciences ,Rapid diagnostic test ,Orientia ,biology ,business.industry ,06 Biological Sciences ,biology.organism_classification ,medicine.disease ,bacterial infections and mycoses ,Virology ,3. Good health ,culture ,Scrub Typhus ,Laos ,business ,isolation - Abstract
In the Lao People’s Democratic Republic (Laos), rickettsial infections, including scrub and murine typhus, account for a significant burden of fevers. The Mahosot Hospital Microbiology Laboratory in Vientiane, Laos, routinely performs rickettsial isolation from hospitalized patients with suspected rickettsioses using mammalian cell culture systems. We review the clinical and laboratory factors associated with successful Orientia tsutsugamushi and Rickettsia typhi isolations from this laboratory over a period of 6 years between 2008 and 2014., In the Lao People’s Democratic Republic (Laos), rickettsial infections, including scrub and murine typhus, account for a significant burden of fevers. The Mahosot Hospital Microbiology Laboratory in Vientiane, Laos, routinely performs rickettsial isolation from hospitalized patients with suspected rickettsioses using mammalian cell culture systems. We review the clinical and laboratory factors associated with successful Orientia tsutsugamushi and Rickettsia typhi isolations from this laboratory over a period of 6 years between 2008 and 2014. The overall isolation success was 7.9% for all samples submitted and 17.3% for samples for which the patient had a positive O. tsutsugamushi or R. typhi rapid diagnostic test (RDT), serology, or PCR. The frequency of successful isolation was highest for samples submitted in November, at the end of the wet season (28.3%). A longer median duration of reported illness, a positive result for a concurrent Orientia or Rickettsia spp. quantitative PCR, and the use of antibiotics by the patient in the week before admission were significantly associated with isolation success (P
- Published
- 2020