1. Role of dendritic cells in the pathogenesis of Whipple's disease.
- Author
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Schinnerling K, Geelhaar-Karsch A, Allers K, Friebel J, Conrad K, Loddenkemper C, Kühl AA, Erben U, Ignatius R, Moos V, and Schneider T
- Subjects
- Adult, Aged, Aged, 80 and over, Antigens, CD biosynthesis, B7-2 Antigen biosynthesis, CD11c Antigen biosynthesis, Cell Adhesion Molecules biosynthesis, Cell Proliferation, Duodenum immunology, Duodenum microbiology, Female, Flow Cytometry, Humans, Immunoglobulins biosynthesis, Interleukin-12 Subunit p35 immunology, Interleukin-12 Subunit p35 metabolism, Lectins, C-Type biosynthesis, Lymph Nodes immunology, Lymphocyte Activation immunology, Macrophages immunology, Macrophages microbiology, Male, Membrane Glycoproteins biosynthesis, Middle Aged, Receptors, CCR7 biosynthesis, Receptors, Cell Surface biosynthesis, Tropheryma immunology, Tropheryma pathogenicity, Whipple Disease microbiology, Whipple Disease mortality, CD83 Antigen, Dendritic Cells immunology, Interleukin-12 Subunit p35 biosynthesis, Th1 Cells immunology, Whipple Disease immunology
- Abstract
Accumulation of Tropheryma whipplei-stuffed macrophages in the duodenum, impaired T. whipplei-specific Th1 responses, and weak secretion of interleukin-12 (IL-12) are hallmarks of classical Whipple's disease (CWD). This study addresses dendritic cell (DC) functionality during CWD. We documented composition, distribution, and functionality of DC ex vivo or after in vitro maturation by fluorescence-activated cell sorting (FACS) and by immunohistochemistry in situ. A decrease in peripheral DC of untreated CWD patients compared to healthy donors was due to reduced CD11c(high) myeloid DC (M-DC). Decreased maturation markers CD83, CD86, and CCR7, as well as low IL-12 production in response to stimulation, disclosed an immature M-DC phenotype. In vitro-generated monocyte-derived DC from CWD patients showed normal maturation and T cell-stimulatory capacity under proinflammatory conditions but produced less IL-12 and failed to activate T. whipplei-specific Th1 cells. In duodenal and lymphoid tissues, T. whipplei was found within immature DC-SIGN(+) DC. DC and proliferating lymphocytes were reduced in lymph nodes of CWD patients compared to levels in controls. Our results indicate that dysfunctional IL-12 production by DC provides suboptimal conditions for priming of T. whipplei-specific T cells during CWD and that immature DC carrying T. whipplei contribute to the dissemination of the bacterium., (Copyright © 2015, American Society for Microbiology. All Rights Reserved.)
- Published
- 2015
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