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Your search keyword '"Rachek, Lyudmila I."' showing total 2 results
Start Over Author "Rachek, Lyudmila I." Publisher american society for microbiology
2 results on '"Rachek, Lyudmila I."'

1. Transformation of Rickettsia prowazekii to rifampin resistance

Author

Rachek, Lyudmila I., Tucker, Aimee M., Winkler, Herbert H., and Wood, David O.

Subjects
Rickettsia -- Genetic aspects, Drug resistance in microorganisms -- Genetic aspects, Bacterial transformation -- Methods, Biological sciences
Abstract

Genetic transformation of the epidemic typhus-causing intracellular pathogen Ricketssia prowazekii was effected by electroporation. The target gene was rpoB encoding the beta subunit of RNA polymerase. The mutant gene inserted in the transformed Rickettsia was the rifampin-resistant RNA polymerase gene. Transformation was effected through homologous recombination. This is the first report of a genetic system in R. prowazekii.

Published
1998

2. Transformation of Rickettsia prowazekii to erythromycin resistance encoded by the Escherichia coli ereB gene

Author

Rachek, Lyudmila I., Hines, Andria, Tucker, Aimee M., Winkler, Herbert H., and Wood, David O.

Subjects
Bacteriology -- Research, Escherichia coli -- Research, Eukaryotic cells -- Analysis, Erythromycin -- Research, Gene expression -- Research, Biological sciences
Abstract

Research has been conducted on the etiologic agent of epidemic typhus Rickettsia prowazekii. The expression of Escherichia coli ereB gene in this parasitic bacterium has been performed.

Published
2000

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