1. A fast and sensitive method for detecting specific viral RNA in mammalian cells
- Author
-
P R De Stasio, Milton W. Taylor, and U Paeratakul
- Subjects
Simplexvirus ,food.ingredient ,Transcription, Genetic ,viruses ,Immunology ,Biology ,Microbiology ,Cell Line ,Nucleic acid thermodynamics ,food ,Interferon ,Viral entry ,Virology ,Viral structural protein ,medicine ,Animals ,Humans ,Encephalomyocarditis virus ,Vero Cells ,Regulation of gene expression ,fungi ,Nucleic Acid Hybridization ,food and beverages ,Gene Expression Regulation ,Glutaral ,Cell culture ,Insect Science ,DNA, Viral ,Vero cell ,RNA, Viral ,Interferons ,Research Article ,medicine.drug - Abstract
A quick and sensitive method to quantitate viral RNA synthesis has been developed. Utilizing glutaraldehyde to fix infected cells onto nitrocellulose paper, viral RNA can be probed directly in situ. Viral message can be detected from as few as 10(4) infected cells. This technique can be used to study viral gene expression and can be adapted to screen the activity of antiviral agents such as interferon.
- Published
- 1988