1. Analysis of Genes Encoding Penicillin-Binding Proteins in Clinical Isolates of Acinetobacter baumannii
- Author
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María-Cruz Rodríguez, Jordi Vila, Felipe Fernández-Cuenca, Álvaro Pascual, Juan A. Ayala, Paula Espinal, Luis Martínez-Martínez, Rodrigo Cayô, Alain A. Ocampo-Sosa, Instituto de Salud Carlos III, Red Española de Investigación en Patología Infecciosa, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Ministerio de Economía y Competitividad (España), [Cayô,R, Rodríguez,MC, Ocampo-Sosa,AA, Martínez-Martínez,L] Service of Microbiology, University Hospital Marqués de Valdecilla—IFIMAV [Espinal,P, Vila,J] Service of Microbiology, Hospital Clinic, School of Medicine, University of Barcelona, Barcelona. [Fernández-Cuenca,F, Pascual,A] Service of Microbiology, University Hospital Virgen Macarena, Seville. [Ayala,JA] Centro de Biología Molecular Severo Ochoa—CSIC-UAM, Campus de Cantoblanco, Madrid, Spain. [Rodríguez,MC, Martínez-Martínez,L] Department of Molecular Biology, School of Medicine, University of Cantabria, Santander., and This work was partially supported by the Instituto de Salud Carlos III, Spanish Network for Research in Infectious Diseases (REIPI, RD06/0008), and FIS (PI080209). We are grateful to the Coordenacao de Aperfeicoamento de Pessoal de Nível Superior (CAPES), which gave a PDEE grant to R.C. (protocol 4149/08-4). We acknowledge the funding of MICINN (BFU2009-09200) to J.A.A. L. Dijkshoorn is thanked for providing A. baumannii strains RUH-134 and RUH-875.
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Acinetobacter baumannii ,Penicillin binding proteins ,Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings] ,polycyclic compounds ,Pharmacology (medical) ,Insertion sequence ,Peptide sequence ,Geographicals::Geographic Locations::Europe::Spain [Medical Subject Headings] ,Genetics ,biology ,Phenomena and Processes::Microbiological Phenomena::Bacterial Physiological Phenomena::Drug Resistance, Bacterial::beta-Lactam Resistance [Medical Subject Headings] ,Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Penicillin-Binding Proteins [Medical Subject Headings] ,Anti-Bacterial Agents ,Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Microbiological Techniques::Microbial Sensitivity Tests [Medical Subject Headings] ,Infectious Diseases ,Phenomena and Processes::Genetic Phenomena::Genetic Variation::Mutation::Point Mutation [Medical Subject Headings] ,Chemicals and Drugs::Organic Chemicals::Amides::Lactams::beta-Lactams::Carbapenems [Medical Subject Headings] ,Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Therapeutic Uses::Anti-Infective Agents::Anti-Bacterial Agents [Medical Subject Headings] ,Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Sequence Analysis::Sequence Analysis, DNA [Medical Subject Headings] ,Acinetobacter Infections ,Silent mutation ,Sequence analysis ,Molecular Sequence Data ,Health Care::Health Care Facilities, Manpower, and Services::Health Facilities::Academic Medical Centers::Hospitals, Teaching [Medical Subject Headings] ,Análisis de Secuencia de ADN ,Microbial Sensitivity Tests ,beta-Lactam Resistance ,Microbiology ,Secuencia de Aminoácidos ,Organisms::Bacteria::Gram-Negative Bacteria::Gram-Negative Aerobic Bacteria::Gram-Negative Aerobic Rods and Cocci::Moraxellaceae::Acinetobacter::Acinetobacter baumannii [Medical Subject Headings] ,Diseases::Bacterial Infections and Mycoses::Bacterial Infections::Gram-Negative Bacterial Infections::Moraxellaceae Infections::Acinetobacter Infections [Medical Subject Headings] ,Mechanisms of Resistance ,Consensus sequence ,Humans ,Penicillin-Binding Proteins ,Point Mutation ,Amino Acid Sequence ,Hospitals, Teaching ,Gene ,Pharmacology ,Proteínas de Unión a las Penicilinas ,Hospitales Escuela ,Sequence Analysis, DNA ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,Information Science::Information Science::Information Services::Documentation::Molecular Sequence Data [Medical Subject Headings] ,Carbapenems ,Spain ,Resistencia beta-Lactámica ,Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Molecular Structure::Amino Acid Sequence [Medical Subject Headings] ,Pruebas de Sensibilidad Microbiana - Abstract
et al., There is limited information on the role of penicillin-binding proteins (PBPs) in the resistance of Acinetobacter baumannii to β-lactams. This study presents an analysis of the allelic variations of PBP genes in A. baumannii isolates. Twenty-six A. baumannii clinical isolates (susceptible or resistant to carbapenems) from three teaching hospitals in Spain were included. The antimicrobial susceptibility profile, clonal pattern, and genomic species identification were also evaluated. Based on the six complete genomes of A. baumannii, the PBP genes were identified, and primers were designed for each gene. The nucleotide sequences of the genes identified that encode PBPs and the corresponding amino acid sequences were compared with those of ATCC 17978. Seven PBP genes and one monofunctional transglycosylase (MGT) gene were identified in the six genomes, encoding (i) four high-molecular-mass proteins (two of class A, PBP1a [ponA] and PBP1b [mrcB], and two of class B, PBP2 [pbpA or mrdA] and PBP3 [ftsI]), (ii) three low-molecular-mass proteins (two of type 5, PBP5/6 [dacC] and PBP6b [dacD], and one of type 7 (PBP7/8 [pbpG]), and (iii) a monofunctional enzyme (MtgA [mtgA]). Hot spot mutation regions were observed, although most of the allelic changes found translated into silent mutations. The amino acid consensus sequences corresponding to the PBP genes in the genomes and the clinical isolates were highly conserved. The changes found in amino acid sequences were associated with concrete clonal patterns but were not directly related to susceptibility or resistance to β-lactams. An insertion sequence disrupting the gene encoding PBP6b was identified in an endemic carbapenem-resistant clone in one of the participant hospitals., This work was partially supported by the Instituto de Salud Carlos III, Spanish Network for Research in Infectious Diseases (REIPI, RD06/0008), and FIS (PI080209). We are grateful to the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), which gave a PDEE grant to R.C. (protocol 4149/08-4). We acknowledge the funding of MICINN (BFU2009-09200) to J.A.A.
- Published
- 2011