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Your search keyword '"Nagashima, Kunio"' showing total 41 results
Start Over Author "Nagashima, Kunio" Publisher american society for microbiology
41 results on '"Nagashima, Kunio"'

4. Restricted Replication of Xenotropic Murine Leukemia Virus-Related Virus in Pigtailed Macaques

Author

Del Prete, Gregory Q., primary, Kearney, Mary F., additional, Spindler, Jon, additional, Wiegand, Ann, additional, Chertova, Elena, additional, Roser, James D., additional, Estes, Jacob D., additional, Hao, Xing Pei, additional, Trubey, Charles M., additional, Lara, Abigail, additional, Lee, KyeongEun, additional, Chaipan, Chawaree, additional, Bess, Julian W., additional, Nagashima, Kunio, additional, Keele, Brandon F., additional, Macallister, Rhonda, additional, Smedley, Jeremy, additional, Pathak, Vinay K., additional, KewalRamani, Vineet N., additional, Coffin, John M., additional, and Lifson, Jeffrey D., additional

Published
2012
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5. Innate Immunity against Granulibacter bethesdensis, an Emerging Gram-Negative Bacterial Pathogen

Author

Zarember, Kol A., primary, Marshall-Batty, Kimberly R., additional, Cruz, Anna R., additional, Chu, Jessica, additional, Fenster, Michael E., additional, Shoffner, Adam R., additional, Rogge, Larissa S., additional, Whitney, Adeline R., additional, Czapiga, Meggan, additional, Song, Helen H., additional, Shaw, Pamela A., additional, Nagashima, Kunio, additional, Malech, Harry L., additional, DeLeo, Frank R., additional, Holland, Steven M., additional, Gallin, John I., additional, and Greenberg, David E., additional

Published
2012
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8. The TY3 Gag3 Spacer Controls Intracellular Condensation and Uncoating

Author

Clemens, Kristina, primary, Larsen, Liza, additional, Zhang, Min, additional, Kuznetsov, Yurii, additional, Bilanchone, Virginia, additional, Randall, Arlo, additional, Harned, Adam, additional, DaSilva, Rhonda, additional, Nagashima, Kunio, additional, McPherson, Alexander, additional, Baldi, Pierre, additional, and Sandmeyer, Suzanne, additional

Published
2011
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14. Ty3 Nucleocapsid Controls Localization of Particle Assembly

Author

Larsen, Liza S. Z., primary, Beliakova-Bethell, Nadejda, additional, Bilanchone, Virginia, additional, Zhang, Min, additional, Lamsa, Anne, additional, DaSilva, Rhonda, additional, Hatfield, G. Wesley, additional, Nagashima, Kunio, additional, and Sandmeyer, Suzanne, additional

Published
2008
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17. Ty3 Capsid Mutations Reveal Early and Late Functions of the Amino-Terminal Domain

Author

Larsen, Liza S. Z., primary, Zhang, Min, additional, Beliakova-Bethell, Nadejda, additional, Bilanchone, Virginia, additional, Lamsa, Anne, additional, Nagashima, Kunio, additional, Najdi, Rani, additional, Kosaka, Kathryn, additional, Kovacevic, Vuk, additional, Cheng, Jianlin, additional, Baldi, Pierre, additional, Hatfield, G. Wesley, additional, and Sandmeyer, Suzanne, additional

Published
2007
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19. Proteomic and Biochemical Analysis of Purified Human Immunodeficiency Virus Type 1 Produced from Infected Monocyte-Derived Macrophages

Author

Chertova, Elena, primary, Chertov, Oleg, additional, Coren, Lori V., additional, Roser, James D., additional, Trubey, Charles M., additional, Bess, Julian W., additional, Sowder, Raymond C., additional, Barsov, Eugene, additional, Hood, Brian L., additional, Fisher, Robert J., additional, Nagashima, Kunio, additional, Conrads, Thomas P., additional, Veenstra, Timothy D., additional, Lifson, Jeffrey D., additional, and Ott, David E., additional

Published
2006
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21. Functional Correlation between a Novel Amino Acid Insertion at Codon 19 in the Protease of Human Immunodeficiency Virus Type 1 and Polymorphism in the p1/p6 Gag Cleavage Site in Drug Resistance and Replication Fitness

Author

Brann, Terrence W., primary, Dewar, Robin L., additional, Jiang, Min-Kan, additional, Shah, Akram, additional, Nagashima, Kunio, additional, Metcalf, Julia A., additional, Falloon, Judith, additional, Lane, H. Clifford, additional, and Imamichi, Tomozumi, additional

Published
2006
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39. Cooperative Effect of Gag Proteins p12 and Capsid during Early Events of Murine Leukemia Virus Replication.

Author

Sook-Kyung Lee, Nagashima, Kunio, and Wei-Shau Hu

Subjects
*MOUSE leukemia viruses, *RETROVIRUSES, *ONCOGENIC viruses, *RNA viruses, *MIRIDAE, *VIRAL replication, *VIROLOGY, *MICROBIOLOGY
Abstract

The Gag polyprutein of murine leukemia virus (MLV) is processed into matrix (MA), p12, capsid (CA), and nucleocapsid (NC) proteins, p12 affects early events of virus replication and contains a PPPY motif important for virus release. To probe the functions of p12 in the early steps of MLV replication, we tested whether p12 can be replaced by spleen necrosis virus (SNV) p18, human immunodeficiency virus type 1 p6, or Rous sarcoma virus p2b. Analyses revealed that all chimeras generated virions at levels similar to that of MLV gag-pol; however, none of them could support MLV vector replication, and all of them exhibited severely reduced DNA synthesis upon virus infection. Because a previously reported SNV gag-MLV pol chimera, but not the MLV hybrid with SNV p18, can support replication of an MLV vector, we hypothesized that other Gag proteins act cooperatively with p12 during the early phase of virus replication. To test this hypothesis, we generated three more MLV-based chimeras containing SNV CA, p18-CA, or p18-CA-NC. We found that the MLV chimera containing SNV p18-CA or p18-CA-NC could support MLV vector replication, but the chimera containing SNV CA could not. Furthermore, viruses derived from the MLV chimera with SNV CA could synthesize viral DNA upon infection but were blocked at a post-reverse-transcription step and generated very little two long terminal repeat circle DNA, thereby producing a phenotype similar to that of the provirus formation-defective p12 mutants. Taken together, our data indicate that when p12/p18 or CA was from different viruses, despite abundant virus production and proper Gag processing, the resulting viruses were not infectious. However, when p12/p18 and CA were from the same virus, even though they were from SNV and not MLV, the resulting viruses were infectious. Therefore, these results suggest a cooperative effect of p12 and CA during the early events of MLV replication. [ABSTRACT FROM AUTHOR]

Published
2005
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40. Quantitation of HLA Class II Protein Incorporated into Human Immunodeficiency Types 1 Virions Purified by Anti-CD45 Immunodeficiency Type 1 Virions Purified by Anti-CD45 Immunoaffinity Depletion of Microvesicles.

Author

Trubey, Charles M., Chertova, Elena, Coren, Lori V., Hilburn, Joanne M., Hixson, Catherine V., Nagashima, Kunio, Lifson, Jeffrey D., and Ott, David E.

Subjects
*HIV, *VIRAL proteins, *VIRAL genetics
Abstract

Among the many host cell-derived proteins found in human immunodeficiency virus type 1 (HIV-1), HLA class II (HLA-II) appears to be selectively incorporated onto virions and may contribute to mechanisms of indirect imunopathogenesis in HIV infection and AIDS. However, the amount of HLA-II on the surface of HIV-1 particles has not been reliably determined due to contamination of virus preparations by microvesicles containing host cell proteins, including HLA-II. Even rigorous sucrose density centrifugation is unable to completely separate HIV-1 from microvesicles. CD45, a leukocyte integral membrane protein, is found on microvesicles, yet appears to be excluded from HIV-1 particles. Exploiting this observation, we have developed a CD45-based immunoaffinity depletion method for removing CD45-containing microvesicles that yields highly purified preparations of virions. Examination of CD45-depleted HIV-1[sub MN] by high-pressure liquid chromatography, protein sequencing, and amino acid analyses determined a molar ratio of HLA-II to Gag of 0.04 to 0.05 in the purified virions, corresponding to an estimated average of 50 to 63 native HLA-II complexes (i.e., a dimer of α and β heterodimers) per virion. These values are approximately 5- to 10-fold lower than those previously determined for other virion preparations that contained microvesicles. Our observations demonstrate the utility of CD45 immunoaffinity-based approaches for producing highly purified retrovirus preparations for applications that would benefit from the use of virus that is essentially free of microvesicles. [ABSTRACT FROM AUTHOR]

Published
2003
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41. Restricted Replication of Xenotropic Murine Leukemia Virus-Related Virus in Pigtailed Macaques.

Author

Prete, Gregory Q. Del, Kearney, Mary F., Spindler, Jon, Wiegand, Ann, Chertova, Elena, Roser, James D., Estes, Jacob D., Xing Pei Hao, Trubey, Charles M., Lara, Abigail, KyeongEun Lee, Chaipan, Chawaree, Bess Jr., Julian W., Nagashima, Kunio, Keele, Brandon F., Macallister, Rhonda, Smedley, Jeremy, Pathak, Vinay K., KewalRamani, Vineet N., and Coffin, John M.

Subjects
*MOUSE leukemia viruses, *VIRAL replication, *DNA viruses, *CELLULAR immunity, *IN situ hybridization, *CELL membranes, *MOUSE diseases, *VIRUS diseases
Abstract

Although xenotropic murine leukemia virus-related virus (XMRV) has been previously linked to prostate cancer and myalgic encephalomyelitis/chronic fatigue syndrome, recent data indicate that results interpreted as evidence of human XMRV infection reflect laboratory contamination rather than authentic in vivo infection. Nevertheless, XMRV is a retrovirus of undefined pathogenic potential that is able to replicate in human cells. Here we describe a comprehensive analysis of two male pigtailed macaques (Macaca nemestrina) experimentally infected with XMRV. Following intravenous inoculation with >1010 RNA copy equivalents of XMRV, viral replication was limited and transient, peaking at ≤2,200 viral RNA (vRNA) copies/ml plasma and becoming undetectable by 4 weeks postinfection, though viral DNA (vDNA) in peripheral blood mononuclear cells remained detectable through 119 days of follow-up. Similarly, vRNA was not detectable in lymph nodes by in situ hybridization despite detectable vDNA. Sequencing of cell-associated vDNA revealed extensive G-to-A hypermutation, suggestive of APOBEC-mediated viral restriction. Consistent with limited viral replication, we found transient upregulation of type I interferon responses that returned to baseline by 2 weeks postinfection, no detectable cellular immune responses, and limited or no spread to prostate tissue. Antibody responses, including neutralizing antibodies, however, were detectable by 2 weeks postinfection and maintained throughout the study. Both animals were healthy for the duration of follow-up. These findings indicate that XMRV replication and spread were limited in pigtailed macaques, predominantly by APOBEC-mediated hypermutation. Given that human APOBEC proteins restrict XMRV infection in vitro, human XMRV infection, if it occurred, would be expected to be characterized by similarly limited viral replication and spread. [ABSTRACT FROM AUTHOR]

Published
2012
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