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Your search keyword '"Koh, Tse-Hsien"' showing total 34 results
Start Over Author "Koh, Tse-Hsien" Publisher american society for microbiology
34 results on '"Koh, Tse-Hsien"'

4. Determining the Development of Persisters in Extensively Drug-Resistant Acinetobacter baumannii upon Exposure to Polymyxin B-Based Antibiotic Combinations Using Flow Cytometry

Author

Wong, Fiona Hui-Sian, primary, Cai, Yiying, additional, Leck, Hui, additional, Lim, Tze-Peng, additional, Teo, Jocelyn Qi-Min, additional, Lee, Winnie, additional, Koh, Tse Hsien, additional, Tan, Thuan Tong, additional, Tan, Kar Wai, additional, and Kwa, Andrea Lay-Hoon, additional

Published
2020
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8. Evaluating Polymyxin B-Based Combinations against Carbapenem-Resistant Escherichia coli in Time-Kill Studies and in a Hollow-Fiber Infection Model

Author

Cai, Yiying, primary, Lim, Tze-Peng, additional, Teo, Jocelyn Qi-Min, additional, Sasikala, Suranthran, additional, Chan, Eric Chun Yong, additional, Hong, Yan Jun, additional, Lee, Winnie, additional, Tan, Thean Yen, additional, Tan, Thuan Tong, additional, Koh, Tse Hsien, additional, Hsu, Li Yang, additional, and Kwa, Andrea L., additional

Published
2017
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11. In Vitro Activity of Polymyxin B in Combination with Various Antibiotics against Extensively Drug-Resistant Enterobacter cloacae with Decreased Susceptibility to Polymyxin B

Author

Cai, Yiying, primary, Lim, Tze-Peng, additional, Teo, Jocelyn, additional, Sasikala, Suranthran, additional, Lee, Winnie, additional, Hong, Yanjun, additional, Chan, Eric Chun Yong, additional, Tan, Thean Yen, additional, Tan, Thuan-Tong, additional, Koh, Tse Hsien, additional, Hsu, Li Yang, additional, and Kwa, Andrea L., additional

Published
2016
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14. In Vitro Activities of Ceftazidime-Avibactam, Aztreonam-Avibactam, and a Panel of Older and Contemporary Antimicrobial Agents against Carbapenemase-Producing Gram-Negative Bacilli

Author

Vasoo, Shawn, primary, Cunningham, Scott A., additional, Cole, Nicolynn C., additional, Kohner, Peggy C., additional, Menon, Sanjay R., additional, Krause, Kevin M., additional, Harris, Kelly A., additional, De, Partha P., additional, Koh, Tse Hsien, additional, and Patel, Robin, additional

Published
2015
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16. In Vitro Pharmacodynamics of Various Antibiotics in Combination against Extensively Drug-Resistant Klebsiella pneumoniae

Author

Lim, Tze-Peng, primary, Cai, Yiying, additional, Hong, Yanjun, additional, Chan, Eric Chun Yong, additional, Suranthran, Sasikala, additional, Teo, Jocelyn Qi-Min, additional, Lee, Winnie Huiling, additional, Tan, Thean-Yen, additional, Hsu, Li-Yang, additional, Koh, Tse-Hsien, additional, Tan, Thuan-Tong, additional, and Kwa, Andrea Lay-Hoon, additional

Published
2015
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19. Arginine Catabolic Mobile Element in Methicillin-Resistant Staphylococcus aureus (MRSA) Clonal Group ST239-MRSA-III Isolates in Singapore: Implications for PCR-Based Screening Tests

Author

Hon, Pei Yun, primary, Chan, Kian Sing, additional, Holden, Matthew T., additional, Harris, Simon R., additional, Tan, Thean Yen, additional, Zu, Yan-Bing, additional, Krishnan, Prabha, additional, Oon, Lynette L., additional, Koh, Tse Hsien, additional, and Hsu, Li Yang, additional

Published
2013
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30. Antecedent Carbapenem Exposure as a Risk Factor for Non-Carbapenemase-Producing Carbapenem-Resistant Enterobacteriaceae and Carbapenemase-Producing Enterobacteriaceae.

Author

Marimuthu K, Ng OT, Cherng BPZ, Fong RKC, Pada SK, De PP, Ooi ST, Smitasin N, Thoon KC, Krishnan PU, Ang MLT, Chan DSG, Kwa ALH, Deepak RN, Chan YK, Chan YFZ, Huan X, Zaw Linn K, Tee NWS, Tan TY, Koh TH, Lin RTP, Hsu LY, Sengupta S, Paterson DL, Perencevich E, Harbarth S, Teo J, and Venkatachalam I

Subjects
Carbapenem-Resistant Enterobacteriaceae drug effects, Carbapenem-Resistant Enterobacteriaceae metabolism, Case-Control Studies, Enterobacteriaceae metabolism, Enterobacteriaceae Infections drug therapy, Escherichia coli drug effects, Female, Humans, Klebsiella pneumoniae drug effects, Male, Microbial Sensitivity Tests methods, Risk Factors, Anti-Bacterial Agents adverse effects, Bacterial Proteins metabolism, Carbapenems adverse effects, Enterobacteriaceae drug effects, beta-Lactamases metabolism
Abstract

Carbapenem-resistant Enterobacteriaceae (CRE) can be mechanistically classified into carbapenemase-producing Enterobacteriaceae (CPE) and non-carbapenemase-producing carbapenem nonsusceptible Enterobacteriaceae (NCPCRE). We sought to investigate the effect of antecedent carbapenem exposure as a risk factor for NCPCRE versus CPE. Among all patients with CRE colonization and infection, we conducted a case-control study comparing patients with NCPCRE (cases) and patients with CPE (controls). The presence of carbapenemases was investigated with phenotypic tests followed by PCR for predominant carbapenemase genes. We included 843 unique patients with first-episode CRE, including 387 (45.9%) NCPCRE and 456 (54.1%) CPE. The resistance genes detected in CPEs were bla NDM (42.8%), bla KPC (38.4%), and bla OXA-48-like (12.1%). After adjusting for confounders and clustering at the institutional level, the odds of prior 30-day carbapenem exposure was three times higher among NCPCRE than CPE patients (adjusted odds ratio [aOR], 3.48; 95% confidence interval [CI], 2.39 to 5.09; P  < 0.001). The odds of prior carbapenem exposure and NCPCRE detection persisted in stratified analyses by Enterobacteriaceae species ( Klebsiella pneumoniae and Escherichia coli ) and carbapenemase gene ( bla NDM and bla KPC ). CPE was associated with male gender (aOR, 1.45; 95% CI, 1.07 to 1.97; P  = 0.02), intensive care unit stay (aOR, 1.84; 95% CI, 1.24 to 2.74; P  = 0.003), and hospitalization in the preceding 1 year (aOR, 1.42; 95% CI, 1.01 to 2.02; P  = 0.05). In a large nationwide study, antecedent carbapenem exposure was a significant risk factor for NCPCRE versus CPE, suggesting a differential effect of antibiotic selection pressure., (Copyright © 2019 American Society for Microbiology.)

Published
2019
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31. Evaluating Polymyxin B-Based Combinations against Carbapenem-Resistant Escherichia coli in Time-Kill Studies and in a Hollow-Fiber Infection Model.

Author

Cai Y, Lim TP, Teo JQ, Sasikala S, Chan EC, Hong YJ, Lee W, Tan TY, Tan TT, Koh TH, Hsu LY, and Kwa AL

Subjects
Drug Resistance, Bacterial genetics, Escherichia coli enzymology, Microbial Sensitivity Tests, Minocycline analogs & derivatives, Minocycline pharmacology, Tigecycline, Anti-Bacterial Agents pharmacology, Carbapenems pharmacology, Escherichia coli drug effects, Polymyxin B pharmacology
Abstract

Polymyxin B-based combinations have emerged as a mainstay treatment against carbapenem-resistant Escherichia coli (CREC). We investigated the activity of polymyxin B-based two-antibiotic combinations against CREC using time-kill studies (TKS) and validated the findings in a hollow-fiber infection model (HFIM). TKS were conducted using 5 clinical CREC strains at 5 log 10 CFU/ml against 10 polymyxin B-based two-antibiotic combinations at maximum clinically achievable concentrations. HFIMs simulating dosing regimens with polymyxin B (30,000U/kg/day) and tigecycline (100 mg every 12 h) alone and in combination were conducted against two CREC strains at 5 log 10 CFU/ml over 120 h. Emergence of resistance was quantified using antibiotic-containing media. Phenotypic characterization (growth rate and stability of resistant phenotypes) of the resistant isolates was performed. All five CREC strains harbored carbapenemases. Polymyxin B and tigecycline MICs ranged from 0.5 mg/liter to 2 mg/liter and from 0.25 mg/liter to 8 mg/liter, respectively. All antibiotics alone did not have bactericidal activity at 24 h in the TKS, except for polymyxin B against two strains. In combination TKS, only polymyxin B plus tigecycline demonstrated both bactericidal activity and synergy in two out of five strains. In the HFIM, polymyxin B alone was bactericidal against both CREC strains before regrowth was observed at 8 h. Phenotypically stable polymyxin B-resistant mutants were observed for both strains, with a reduced growth rate observed in one strain. Tigecycline alone resulted in a slow reduction in bacterial counts. Polymyxin B plus tigecycline resulted in rapid and sustained bactericidal killing up to 120 h. Polymyxin B plus tigecycline is a promising combination against CREC. The clinical relevance of our results warrants further investigations., (Copyright © 2016 American Society for Microbiology.)

Published
2016
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32. mcr-1 in Multidrug-Resistant blaKPC-2-Producing Clinical Enterobacteriaceae Isolates in Singapore.

Author

Teo JQ, Ong RT, Xia E, Koh TH, Khor CC, Lee SJ, Lim TP, and Kwa AL

Subjects
Bacterial Proteins genetics, Drug Resistance, Bacterial, Enterobacteriaceae drug effects, Enterobacteriaceae genetics, Enterobacteriaceae isolation & purification, Humans, Plasmids genetics, Sequence Analysis, DNA, Singapore, Anti-Bacterial Agents pharmacology, Enterobacteriaceae enzymology, Enterobacteriaceae Infections microbiology, Genome, Bacterial genetics, Polymyxins pharmacology, beta-Lactamases genetics
Published
2016
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33. Multilocus sequence types of carbapenem-resistant Pseudomonas aeruginosa in Singapore carrying metallo-beta-lactamase genes, including the novel bla(IMP-26) gene.

Author

Koh TH, Khoo CT, Tan TT, Arshad MA, Ang LP, Lau LJ, Hsu LY, and Ooi EE

Subjects
Bacterial Typing Techniques, Electrophoresis, Gel, Pulsed-Field, Genotype, Humans, Molecular Sequence Data, Sequence Analysis, DNA methods, Singapore, beta-Lactam Resistance genetics, Bacterial Proteins genetics, Carbapenems pharmacology, Pseudomonas aeruginosa drug effects, Pseudomonas aeruginosa genetics, beta-Lactamases genetics
Abstract

Nine imipenem-resistant Pseudomonas aeruginosa isolates were found to contain a variety of metallo-beta-lactamase genes, including bla(IMP-1), bla(IMP-7), bla(VIM-2), bla(VIM-6), and the novel bla(IMP-26). Multilocus sequence typing showed a diversity of sequence types. Comparison with isolates from an earlier study showed that the epidemic clones from 2000 have not become established.

Published
2010
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34. IMP-1 and a novel metallo-beta-lactamase, VIM-6, in fluorescent pseudomonads isolated in Singapore.

Author

Koh TH, Wang GC, and Sng LH

Subjects
Amino Acid Sequence, Blotting, Southern, Conjugation, Genetic, DNA, Bacterial genetics, Drug Resistance, Bacterial, Isoelectric Focusing, Molecular Sequence Data, Plasmids genetics, Pseudomonas Infections microbiology, Pseudomonas fluorescens drug effects, Pseudomonas putida drug effects, Reverse Transcriptase Polymerase Chain Reaction, Singapore, Pseudomonas fluorescens enzymology, Pseudomonas putida enzymology, beta-Lactamases metabolism
Abstract

Four carbapenem-resistant Pseudomonas spp. were isolated from patients in Singapore. One Pseudomonas putida isolate contained a bla(IMP-1) identical to that first described in Japan. The sequence of a variant bla(IMP-1) in Pseudomonas fluorescens contained four silent mutations compared with the original sequence. The remaining P. putida isolates contained bla(VIM-6), a novel VIM gene variant.

Published
2004
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