1. Characterization of CIA-1, an Ambler class A extended-spectrum β-lactamase from Chryseobacterium indologenes.
- Author
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Matsumoto T, Nagata M, Ishimine N, Kawasaki K, Yamauchi K, Hidaka E, Kasuga E, Horiuchi K, Oana K, Kawakami Y, and Honda T
- Subjects
- Amino Acid Sequence, Chromosomes, Bacterial genetics, Chryseobacterium drug effects, Chryseobacterium isolation & purification, Cloning, Molecular, Escherichia coli, Flavobacteriaceae Infections microbiology, Humans, Microbial Sensitivity Tests, Molecular Sequence Data, Recombinant Proteins classification, Recombinant Proteins genetics, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Amino Acid, beta-Lactamases classification, Anti-Bacterial Agents administration & dosage, Chryseobacterium genetics, Flavobacteriaceae Infections drug therapy, beta-Lactam Resistance, beta-Lactamases genetics
- Abstract
An Ambler class A β-lactamase gene, bla(CIA-1), was cloned from the reference strain Chryseobacterium indologenes ATCC 29897 and expressed in Escherichia coli BL21. The bla(CIA-1) gene encodes a novel extended-spectrum β-lactamase (ESBL) that shared 68% and 60% identities with the CGA-1 and CME-1 β-lactamases, respectively. bla(CIA-1)-like genes were detected from clinical isolates. In addition to the metallo-β-lactamase IND of Ambler class B, C. indologenes has a class A ESBL gene, bla(CIA-1), located on the chromosome.
- Published
- 2012
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