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Your search keyword '"Ishihara, Kazuyuki"' showing total 13 results
Start Over Author "Ishihara, Kazuyuki" Publisher american society for microbiology
13 results on '"Ishihara, Kazuyuki"'

1. Dentilisin activity affects the organization of the outer sheath of Treponema denticola

Author

Ishihara, Kazuyuki, Kuramitsu, Howard K., Miura, Tadashi, and Okuda, Katsuji

Subjects
Periodontal disease -- Research, Pathogenic microorganisms -- Research, Virulence (Microbiology) -- Research, Bacterial proteins -- Research, Biological sciences
Abstract

Increased levels of the helically shaped microorganism Treponema denticola have been found to parallel the destruction of human periodontal tissue. These and other observations suggest that the microorganism may be a pathogen involved in periodontitis. Proteases are believed to be important variables in periodontal disease. A prtP-deficient mutant was constructed to gauge the physiological function of the protease in the expression of cell surface proteins and its possible function in virulence.

Published
1998

2. Cloning and expression of the aspartate carbamoyltransferase gene from Treponema denticola

Author

Ishihara, Kazuyuki, Ishihara, Michiyo, Takazoe, Ichiro, and Okuda, Katsuji

Subjects
Periodontal disease -- Research, Transferases -- Genetic aspects, Biological sciences
Abstract

An investigation was conducted to clone and characterize antigenic proteins of Treponema denticola, an oral spirochete associated with the subgingival plaque in periodontitis. One clone chosen for further analysis coded for a protein with 33.8% homology to the aspartate carbamoyltransferase (ATCase) of Escherichia coli. The protein-coding region was isolated and expressed in E. coli, and the expressed product was partially purified. Enzyme assays on the partially purified product revealed the presence of ATCase activity. Furthermore, the protein reacted with antisera against T. denticola, demonstrating its antigenicity.

Published
1992

11. Complete genome sequence of Fusobacterium vincentii strain TDC100 isolated from an apical periodontitis lesion.

Author

Yonezawa H, Kokubu E, Kikuchi Y, and Ishihara K

Abstract

This study reports the complete genome sequence of Fusobacterium vincentii strain TDC100. The complete circular chromosome of strain TDC100 was obtained and assembled using a combination of short- and long-read sequencing., Competing Interests: The authors declare no conflict of interest.

Published
2024
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12. The Treponema denticola surface protease dentilisin degrades interleukin-1 beta (IL-1 beta), IL-6, and tumor necrosis factor alpha.

Author

Miyamoto M, Ishihara K, and Okuda K

Subjects
Bacterial Proteins, Chymotrypsin genetics, Electrophoresis, Polyacrylamide Gel, Humans, Immunoblotting, Interleukin-1 genetics, Interleukin-6 genetics, Mutation, Peptide Hydrolases, RNA, Messenger metabolism, Recombinant Proteins genetics, Recombinant Proteins metabolism, Reverse Transcriptase Polymerase Chain Reaction, Treponema denticola genetics, Treponema denticola pathogenicity, Tumor Necrosis Factor-alpha genetics, Chymotrypsin physiology, Interleukin-1 metabolism, Interleukin-6 metabolism, Treponema denticola enzymology, Tumor Necrosis Factor-alpha metabolism
Abstract

Dentilisin is a major surface protease and virulence factor of the bacterium Treponema denticola. In this study, we found that T. denticola reduced inflammatory cytokines, including interleukin-1beta (IL-1beta), IL-6, and tumor necrosis factor alpha, in peripheral blood mononuclear cells through degradation by dentilisin.

Published
2006
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13. Correlation between detection rates of periodontopathic bacterial DNA in coronary stenotic artery plaque [corrected] and in dental plaque samples.

Author

Ishihara K, Nabuchi A, Ito R, Miyachi K, Kuramitsu HK, and Okuda K

Subjects
Aggregatibacter actinomycetemcomitans isolation & purification, Bacteroides isolation & purification, Campylobacter rectus isolation & purification, DNA, Ribosomal genetics, Humans, Porphyromonas gingivalis isolation & purification, RNA, Ribosomal, 16S genetics, Sensitivity and Specificity, Treponema isolation & purification, Bacterial Infections diagnosis, Carotid Stenosis microbiology, DNA, Bacterial isolation & purification, Dental Plaque microbiology, Polymerase Chain Reaction methods
Abstract

Utilizing PCR, the 16S rRNA detection rates for Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans, Bacteroides forsythus, Treponema denticola, and Campylobacter rectus in samples of stenotic coronary artery plaques were determined to be 21.6, 23.3, 5.9, 23.5, and 15.7%, respectively. The detection rates for P. gingivalis and C. rectus correlated with their presence in subgingival plaque.

Published
2004
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