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The different phenotypes of isozymes for 101 strains of Gibberella fujikuroi and two strains of G. nygamai were investigated by isolation and resolution methods. Six enzymes were monomorphic across six of the mating populations while eight enzymes were polymorphic within at least one of the mating populations. The C and D mating populations showed high similarity, while the E population was the least similar to the other populations. Isozyme data revealed that certain biological species share the same phenotypes but are reproductively isolated from one another.