10 results on '"Howard, Wendy"'
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2. The Effect of the PB2 Mutation 627K on Highly Pathogenic H5N1 Avian Influenza Virus Is Dependent on the Virus Lineage
- Author
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Long, Jason S., primary, Howard, Wendy A., additional, Núñez, Alejandro, additional, Moncorgé, Olivier, additional, Lycett, Samantha, additional, Banks, Jill, additional, and Barclay, Wendy S., additional
- Published
- 2013
- Full Text
- View/download PDF
3. A Complicated Message: Identification of a Novel PB1-Related Protein Translated from Influenza A Virus Segment 2 mRNA
- Author
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Wise, Helen M., primary, Foeglein, Agnes, additional, Sun, Jiechao, additional, Dalton, Rosa Maria, additional, Patel, Sheetal, additional, Howard, Wendy, additional, Anderson, Emma C., additional, Barclay, Wendy S., additional, and Digard, Paul, additional
- Published
- 2009
- Full Text
- View/download PDF
4. Protective Immune Responses to the 42-Kilodalton (kDa) Region ofPlasmodium yoeliiMerozoite Surface Protein 1 Are Induced by the C-Terminal 19-kDa Region but Not by the Adjacent 33-kDa Region
- Author
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Ahlborg, Niklas, primary, Ling, Irene T., additional, Howard, Wendy, additional, Holder, Anthony A., additional, and Riley, Eleanor M., additional
- Published
- 2002
- Full Text
- View/download PDF
5. Chicken and duck myotubes are highly susceptible and permissive to influenza virus infection
- Author
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Baquero-Perez, Belinda, Kuchipudi, Suresh V., Ho, Jemima, Sebastian, Sujith, Puranik, Anita, Howard, Wendy, Brookes, Sharon M., Brown, Ian H., Chang, Kin-Chow, Baquero-Perez, Belinda, Kuchipudi, Suresh V., Ho, Jemima, Sebastian, Sujith, Puranik, Anita, Howard, Wendy, Brookes, Sharon M., Brown, Ian H., and Chang, Kin-Chow
- Abstract
Skeletal muscle, at 30 to 40% of body mass, is the most abundant soft tissue in the body. Besides its primary function in movement and posture, skeletal muscle is a significant innate immune organ with the capacity to produce cytokines and chemokines and respond to proinflammatory cytokines. Little is known about the role of skeletal muscle during systemic influenza A virus infection in any host and particularly avian species. Here we used primary chicken and duck multinucleated myotubes to examine their susceptibility and innate immune response to influenza virus infections. Both chicken and duck myotubes expressed avian and human sialic acid receptors and were readily susceptible to low-pathogenicity (H2N3 A/mallard duck/England/7277/06) and high-pathogenicity (H5N1 A/turkey/England/50-92/91 and H5N1 A/turkey/Turkey/1/05) avian and human H1N1 (A/USSR/77) influenza viruses. Both avian host species produced comparable levels of progeny H5N1 A/turkey/Turkey/1/05 virus.Notably, the rapid accumulation of viral nucleoprotein and matrix (M) gene RNA in chicken and duck myotubes was accompanied by extensive cytopathic damage with marked myotube apoptosis (widespread microscopic blebs, caspase 3/7 activation, and annexin V binding at the plasma membrane). Infected chicken myotubes produced significantly higher levels of proinflammatory cytokines than did the corresponding duck cells. Additionally, in chicken myotubes infected with H5N1 viruses, the induction of interferon beta (IFN-beta) and IFN-inducible genes, including the melanoma differentiation-associated protein 5 (MDA-5) gene, was relatively weak compared to infection with the corresponding H2N3 virus. Our findings highlight that avian skeletal muscle fibers are capable of productive influenza virus replication and are a potential tissue source of infection.
- Full Text
- View/download PDF
6. Chicken and duck myotubes are highly susceptible and permissive to influenza virus infection
- Author
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Baquero-Perez, Belinda, Kuchipudi, Suresh V., Ho, Jemima, Sebastian, Sujith, Puranik, Anita, Howard, Wendy, Brookes, Sharon M., Brown, Ian H., Chang, Kin-Chow, Baquero-Perez, Belinda, Kuchipudi, Suresh V., Ho, Jemima, Sebastian, Sujith, Puranik, Anita, Howard, Wendy, Brookes, Sharon M., Brown, Ian H., and Chang, Kin-Chow
- Abstract
Skeletal muscle, at 30 to 40% of body mass, is the most abundant soft tissue in the body. Besides its primary function in movement and posture, skeletal muscle is a significant innate immune organ with the capacity to produce cytokines and chemokines and respond to proinflammatory cytokines. Little is known about the role of skeletal muscle during systemic influenza A virus infection in any host and particularly avian species. Here we used primary chicken and duck multinucleated myotubes to examine their susceptibility and innate immune response to influenza virus infections. Both chicken and duck myotubes expressed avian and human sialic acid receptors and were readily susceptible to low-pathogenicity (H2N3 A/mallard duck/England/7277/06) and high-pathogenicity (H5N1 A/turkey/England/50-92/91 and H5N1 A/turkey/Turkey/1/05) avian and human H1N1 (A/USSR/77) influenza viruses. Both avian host species produced comparable levels of progeny H5N1 A/turkey/Turkey/1/05 virus.Notably, the rapid accumulation of viral nucleoprotein and matrix (M) gene RNA in chicken and duck myotubes was accompanied by extensive cytopathic damage with marked myotube apoptosis (widespread microscopic blebs, caspase 3/7 activation, and annexin V binding at the plasma membrane). Infected chicken myotubes produced significantly higher levels of proinflammatory cytokines than did the corresponding duck cells. Additionally, in chicken myotubes infected with H5N1 viruses, the induction of interferon beta (IFN-beta) and IFN-inducible genes, including the melanoma differentiation-associated protein 5 (MDA-5) gene, was relatively weak compared to infection with the corresponding H2N3 virus. Our findings highlight that avian skeletal muscle fibers are capable of productive influenza virus replication and are a potential tissue source of infection.
- Full Text
- View/download PDF
7. Chicken and duck myotubes are highly susceptible and permissive to influenza virus infection
- Author
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Baquero-Perez, Belinda, Kuchipudi, Suresh V., Ho, Jemima, Sebastian, Sujith, Puranik, Anita, Howard, Wendy, Brookes, Sharon M., Brown, Ian H., Chang, Kin-Chow, Baquero-Perez, Belinda, Kuchipudi, Suresh V., Ho, Jemima, Sebastian, Sujith, Puranik, Anita, Howard, Wendy, Brookes, Sharon M., Brown, Ian H., and Chang, Kin-Chow
- Abstract
Skeletal muscle, at 30 to 40% of body mass, is the most abundant soft tissue in the body. Besides its primary function in movement and posture, skeletal muscle is a significant innate immune organ with the capacity to produce cytokines and chemokines and respond to proinflammatory cytokines. Little is known about the role of skeletal muscle during systemic influenza A virus infection in any host and particularly avian species. Here we used primary chicken and duck multinucleated myotubes to examine their susceptibility and innate immune response to influenza virus infections. Both chicken and duck myotubes expressed avian and human sialic acid receptors and were readily susceptible to low-pathogenicity (H2N3 A/mallard duck/England/7277/06) and high-pathogenicity (H5N1 A/turkey/England/50-92/91 and H5N1 A/turkey/Turkey/1/05) avian and human H1N1 (A/USSR/77) influenza viruses. Both avian host species produced comparable levels of progeny H5N1 A/turkey/Turkey/1/05 virus.Notably, the rapid accumulation of viral nucleoprotein and matrix (M) gene RNA in chicken and duck myotubes was accompanied by extensive cytopathic damage with marked myotube apoptosis (widespread microscopic blebs, caspase 3/7 activation, and annexin V binding at the plasma membrane). Infected chicken myotubes produced significantly higher levels of proinflammatory cytokines than did the corresponding duck cells. Additionally, in chicken myotubes infected with H5N1 viruses, the induction of interferon beta (IFN-beta) and IFN-inducible genes, including the melanoma differentiation-associated protein 5 (MDA-5) gene, was relatively weak compared to infection with the corresponding H2N3 virus. Our findings highlight that avian skeletal muscle fibers are capable of productive influenza virus replication and are a potential tissue source of infection.
- Full Text
- View/download PDF
8. Chicken and duck myotubes are highly susceptible and permissive to influenza virus infection
- Author
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Baquero-Perez, Belinda, Kuchipudi, Suresh V., Ho, Jemima, Sebastian, Sujith, Puranik, Anita, Howard, Wendy, Brookes, Sharon M., Brown, Ian H., Chang, Kin-Chow, Baquero-Perez, Belinda, Kuchipudi, Suresh V., Ho, Jemima, Sebastian, Sujith, Puranik, Anita, Howard, Wendy, Brookes, Sharon M., Brown, Ian H., and Chang, Kin-Chow
- Abstract
Skeletal muscle, at 30 to 40% of body mass, is the most abundant soft tissue in the body. Besides its primary function in movement and posture, skeletal muscle is a significant innate immune organ with the capacity to produce cytokines and chemokines and respond to proinflammatory cytokines. Little is known about the role of skeletal muscle during systemic influenza A virus infection in any host and particularly avian species. Here we used primary chicken and duck multinucleated myotubes to examine their susceptibility and innate immune response to influenza virus infections. Both chicken and duck myotubes expressed avian and human sialic acid receptors and were readily susceptible to low-pathogenicity (H2N3 A/mallard duck/England/7277/06) and high-pathogenicity (H5N1 A/turkey/England/50-92/91 and H5N1 A/turkey/Turkey/1/05) avian and human H1N1 (A/USSR/77) influenza viruses. Both avian host species produced comparable levels of progeny H5N1 A/turkey/Turkey/1/05 virus.Notably, the rapid accumulation of viral nucleoprotein and matrix (M) gene RNA in chicken and duck myotubes was accompanied by extensive cytopathic damage with marked myotube apoptosis (widespread microscopic blebs, caspase 3/7 activation, and annexin V binding at the plasma membrane). Infected chicken myotubes produced significantly higher levels of proinflammatory cytokines than did the corresponding duck cells. Additionally, in chicken myotubes infected with H5N1 viruses, the induction of interferon beta (IFN-beta) and IFN-inducible genes, including the melanoma differentiation-associated protein 5 (MDA-5) gene, was relatively weak compared to infection with the corresponding H2N3 virus. Our findings highlight that avian skeletal muscle fibers are capable of productive influenza virus replication and are a potential tissue source of infection.
- Full Text
- View/download PDF
9. Chicken and duck myotubes are highly susceptible and permissive to influenza virus infection.
- Author
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Baquero-Perez B, Kuchipudi SV, Ho J, Sebastian S, Puranik A, Howard W, Brookes SM, Brown IH, and Chang KC
- Subjects
- Animals, Apoptosis, Cells, Cultured, Chickens, Cytokines metabolism, Cytopathogenic Effect, Viral, Ducks, Gene Expression Profiling, RNA, Messenger biosynthesis, RNA, Viral biosynthesis, Receptors, Virus analysis, Sialic Acids analysis, Influenza A Virus, H1N1 Subtype growth & development, Influenza A Virus, H3N2 Subtype growth & development, Influenza A Virus, H5N1 Subtype growth & development, Muscle Fibers, Skeletal virology
- Abstract
Unlabelled: Skeletal muscle, at 30 to 40% of body mass, is the most abundant soft tissue in the body. Besides its primary function in movement and posture, skeletal muscle is a significant innate immune organ with the capacity to produce cytokines and chemokines and respond to proinflammatory cytokines. Little is known about the role of skeletal muscle during systemic influenza A virus infection in any host and particularly avian species. Here we used primary chicken and duck multinucleated myotubes to examine their susceptibility and innate immune response to influenza virus infections. Both chicken and duck myotubes expressed avian and human sialic acid receptors and were readily susceptible to low-pathogenicity (H2N3 A/mallard duck/England/7277/06) and high-pathogenicity (H5N1 A/turkey/England/50-92/91 and H5N1 A/turkey/Turkey/1/05) avian and human H1N1 (A/USSR/77) influenza viruses. Both avian host species produced comparable levels of progeny H5N1 A/turkey/Turkey/1/05 virus. Notably, the rapid accumulation of viral nucleoprotein and matrix (M) gene RNA in chicken and duck myotubes was accompanied by extensive cytopathic damage with marked myotube apoptosis (widespread microscopic blebs, caspase 3/7 activation, and annexin V binding at the plasma membrane). Infected chicken myotubes produced significantly higher levels of proinflammatory cytokines than did the corresponding duck cells. Additionally, in chicken myotubes infected with H5N1 viruses, the induction of interferon beta (IFN-β) and IFN-inducible genes, including the melanoma differentiation-associated protein 5 (MDA-5) gene, was relatively weak compared to infection with the corresponding H2N3 virus. Our findings highlight that avian skeletal muscle fibers are capable of productive influenza virus replication and are a potential tissue source of infection., Importance: Infection with high-pathogenicity H5N1 viruses in ducks is often asymptomatic, and skeletal muscle from such birds could be a source of infection of humans and animals. Little is known about the ability of influenza A viruses to replicate in avian skeletal muscle fibers. We show here that cultured chicken and duck myotubes were highly susceptible to infection with both low- and high-pathogenicity avian influenza viruses. Infected myotubes of both avian species displayed rapid virus accumulation, apoptosis, and extensive cellular damage. Our results indicate that avian skeletal muscle fibers of chicken and duck could be significant contributors to progeny production of highly pathogenic H5N1 viruses., (Copyright © 2015, Baquero-Perez et al.)
- Published
- 2015
- Full Text
- View/download PDF
10. Protective immune responses to the 42-kilodalton (kDa) region of Plasmodium yoelii merozoite surface protein 1 are induced by the C-terminal 19-kDa region but not by the adjacent 33-kDa region.
- Author
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Ahlborg N, Ling IT, Howard W, Holder AA, and Riley EM
- Subjects
- Animals, Antibodies, Protozoan biosynthesis, Antibodies, Protozoan immunology, Enzyme-Linked Immunosorbent Assay methods, Epitopes, B-Lymphocyte genetics, Female, Fluorescent Antibody Technique, Indirect, Glutathione Transferase genetics, Glutathione Transferase immunology, Merozoite Surface Protein 1 genetics, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Molecular Weight, Recombinant Fusion Proteins genetics, Recombinant Fusion Proteins immunology, Vaccination, Epitopes, B-Lymphocyte immunology, Malaria prevention & control, Merozoite Surface Protein 1 immunology, Plasmodium yoelii immunology
- Abstract
Vaccination of mice with the 42-kDa region of Plasmodium yoelii merozoite surface protein 1 (MSP1(42)) or its 19-kDa C-terminal processing product (MSP1(19)) can elicit protective antibody responses in mice. To investigate if the 33-kDa N-terminal fragment (MSP1(33)) of MSP1(42) also induces protection, the gene segment encoding MSP1(33) was expressed as a glutathione S-transferase (GST) fusion protein. C57BL/6 and BALB/c mice were immunized with GST-MSP1(33) and subsequently challenged with the lethal P. yoelii YM blood stage parasite. GST-MSP1(33) failed to induce protection, and all mice developed patent parasitemia at a level similar to that in naive or control (GST-immunized) mice; mice immunized with GST-MSP1(19) were protected, as has been shown previously. Specific prechallenge immunoglobulin G (IgG) antibody responses to MSP1 were analyzed by enzyme-linked immunosorbent assay and immunofluorescence. Despite being unprotected, several mice immunized with MSP1(33) had antibody titers (of all IgG subclasses) that were comparable to or higher than those in mice that were protected following immunization with MSP1(19). The finding that P. yoelii MSP1(33) elicits strong but nonprotective antibody responses may have implications for the design of vaccines for humans based on Plasmodium falciparum or Plasmodium vivax MSP1(42).
- Published
- 2002
- Full Text
- View/download PDF
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