1. Beta-cyanoalanine formation by Chromobacterium violaceum.
- Author
-
Brysk MM, Corpe WA, and Hankes LV
- Subjects
- Amino Acids analysis, Asparagine biosynthesis, Aspartic Acid biosynthesis, Carbon Isotopes, Formaldehyde metabolism, Glycine metabolism, Methionine metabolism, Serine metabolism, Succinates metabolism, Alanine biosynthesis, Chromobacterium metabolism, Cyanides biosynthesis
- Abstract
Nonproliferating cells of Chromobacterium violaceum incubated with glycine, methionine, and succinate as substrates accumulated beta-cyanoalanine in the culture fluid. Tracer experiments showed that carbons-2, -3, and -4 of beta-cyanoalanine are derived from the 2-carbon of glycine. When methionine-methyl-(14)C, succinate-1,4-(14)C, or succinate-2,3-(14)C was used as substrate, beta-cyanoalanine did not become labeled. If K(14)CN and serine were used as substrates, the cyano group of beta-cyanoalanine was labeled. Radioactive beta-cyanoalanine, labeled in the 3-carbon, was formed when glycine and H(14)CHO were used as substrates. (14)C-formic acid did not replace formaldehyde. Asparagine also accumulated in the incubated mixture and was found to be labeled in the amide carbon. Incubation of cells with beta-cyanoalanine-4-(14)C produced labeled aspartic acid in cell hydrolysates.
- Published
- 1969
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