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In Streptomyces coelicolor ParB is required for accurate chromosome partitioning during sporulation. Using a functional ParB-enhanced green fluorescent protein fusion, we observed bright tip-associated foci and other weaker, irregular foci in S. coelicolor vegetative hyphae. In contrast, in aerial hyphae regularly spaced bright loci accompanied sporulation-associated chromosome condensation and septation.

Researchers used subtractive hybridization between genome-specific DNA and total RNA and PCR to analyze gene expression in Streptomyces coelicolor A3(2) and the accessory linear plasmid SCP1. About 24 genes in the silent regions of S. coelicolor A3(2) chromosomes are expressed.

In vitro study was conducted to understand the role of coexpression Cpz31 in Streptomyces coelicolor for the attachment of the L-rhamnose to the caprazamycins aglycones, generating a rare acylated deoxyhexose. The recombineering approach used might provide a useful tool for the assembly of biosynthetic building blocks for heterologous production of microbial compounds.

An efficient method for the heterologous expression of biosynthetic gene clusters in different Streptomyces strains is described. Lambda-Red-mediated recombination in Escherichia coli is used for the introduction of integration functions into the cosmid backbones, which allowed subsequent site-specific integration into the genome of the heterologous host.