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Your search keyword '"Gasser, Romain"' showing total 9 results
Start Over Author "Gasser, Romain" Publisher american society for microbiology
9 results on '"Gasser, Romain"'

1. Correction for Valcourt et al., “Evaluating Humoral Immunity against SARS-CoV-2: Validation of a Plaque-Reduction Neutralization Test and a Multilaboratory Comparison of Conventional and Surrogate Neutralization Assays”

Author

Valcourt, Emelissa J., primary, Manguiat, Kathy, additional, Robinson, Alyssia, additional, Lin, Yi-Chan, additional, Abe, Kento T., additional, Mubarek, Samira, additional, Shigayev, Altynay, additional, Zhong, Zoë, additional, Girardin, Roxie C., additional, DuPuis, Alan, additional, Payne, Anne, additional, McDonough, Kathleen, additional, Wang, Zhen, additional, Gasser, Romain, additional, Laumaea, Annemarie, additional, Benlarbi, Mehdi, additional, Richard, Jonathan, additional, Prévost, Jérémie, additional, Anand, Sai Priya, additional, Dimitrova, Kristina, additional, Phillipson, Clark, additional, Evans, David H., additional, McGeer, Allison, additional, Gingras, Anne-Claude, additional, Liang, Chen, additional, Petric, Martin, additional, Sekirov, Inna, additional, Morshed, Muhammad, additional, Finzi, Andrés, additional, Drebot, Michael, additional, and Wood, Heidi, additional

Published
2022
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2. Detection of the HIV-1 Accessory Proteins Nef and Vpu by Flow Cytometry Represents a New Tool to Study Their Functional Interplay within a Single Infected CD4 + T Cell

Author

Prévost, Jérémie, primary, Richard, Jonathan, additional, Gasser, Romain, additional, Medjahed, Halima, additional, Kirchhoff, Frank, additional, Hahn, Beatrice H., additional, Kappes, John C., additional, Ochsenbauer, Christina, additional, Duerr, Ralf, additional, and Finzi, Andrés, additional

Published
2022
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3. Evaluating Humoral Immunity against SARS-CoV-2: Validation of a Plaque-Reduction Neutralization Test and a Multilaboratory Comparison of Conventional and Surrogate Neutralization Assays

Author

Valcourt, Emelissa J., primary, Manguiat, Kathy, additional, Robinson, Alyssia, additional, Lin, Yi-Chan, additional, Abe, Kento T., additional, Mubareka, Samira, additional, Shigayeva, Altynay, additional, Zhong, Zoë, additional, Girardin, Roxie C., additional, DuPuis, Alan, additional, Payne, Anne, additional, McDonough, Kathleen, additional, Wang, Zhen, additional, Gasser, Romain, additional, Laumaea, Annemarie, additional, Benlarbi, Mehdi, additional, Richard, Jonathan, additional, Prévost, Jérémie, additional, Anand, Sai Priya, additional, Dimitrova, Kristina, additional, Phillipson, Clark, additional, Evans, David H., additional, McGeer, Allison, additional, Gingras, Anne-Claude, additional, Liang, Chen, additional, Petric, Martin, additional, Sekirov, Inna, additional, Morshed, Muhammad, additional, Finzi, Andrés, additional, Drebot, Michael, additional, and Wood, Heidi, additional

Published
2021
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4. Across Functional Boundaries: Making Nonneutralizing Antibodies To Neutralize HIV-1 and Mediate Fc-Mediated Effector Killing of Infected Cells

Author

Richard, Jonathan, primary, Nguyen, Dung N., additional, Tolbert, William D., additional, Gasser, Romain, additional, Ding, Shilei, additional, Vézina, Dani, additional, Yu Gong, Shang, additional, Prévost, Jérémie, additional, Gendron-Lepage, Gabrielle, additional, Medjahed, Halima, additional, Gottumukkala, Suneetha, additional, Finzi, Andrés, additional, and Pazgier, Marzena, additional

Published
2021
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5. Decline of Humoral Responses against SARS-CoV-2 Spike in Convalescent Individuals

Author

Beaudoin-Bussières, Guillaume, primary, Laumaea, Annemarie, additional, Anand, Sai Priya, additional, Prévost, Jérémie, additional, Gasser, Romain, additional, Goyette, Guillaume, additional, Medjahed, Halima, additional, Perreault, Josée, additional, Tremblay, Tony, additional, Lewin, Antoine, additional, Gokool, Laurie, additional, Morrisseau, Chantal, additional, Bégin, Philippe, additional, Tremblay, Cécile, additional, Martel-Laferrière, Valérie, additional, Kaufmann, Daniel E., additional, Richard, Jonathan, additional, Bazin, Renée, additional, and Finzi, Andrés, additional

Published
2020
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8. CD4 Incorporation into HIV-1 Viral Particles Exposes Envelope Epitopes Recognized by CD4-Induced Antibodies.

Author

Shilei Ding, Gasser, Romain, Gendron-Lepage, Gabrielle, Medjahed, Halima, Tolbert, William D., Sodroski, Joseph, Pazgier, Marzena, and Finzi, Andrés

Subjects
*ANTIBODY-dependent cell cytotoxicity, *NEGATIVE regulatory factor, *IMMUNOGLOBULINS, *EPITOPES, *VIRUS inactivation, *VIRAL antibodies
Abstract

CD4 downregulation on infected cells is a highly conserved function of primate lentiviruses. It has been shown to positively impact viral replication by a variety of mechanisms, including enhanced viral release and infectivity, decrease of cell reinfection, and protection from antibody-dependent cellular cytotoxicity (ADCC), which is often mediated by antibodies that require CD4 to change envelope (Env) conformation. Here, we report that incorporation of CD4 into HIV-1 viral particles affects Env conformation resulting in the exposure of occluded epitopes recognized by CD4-induced antibodies. This translates into enhanced neutralization susceptibility by these otherwise nonneutralizing antibodies but is prevented by the HIV-1 Nef accessory protein. Altogether, these findings suggest that another functional consequence of Nef-mediated CD4 downregulation is the protection of viral particles from neutralization by commonly elicited CD4-induced antibodies. [ABSTRACT FROM AUTHOR]

Published
2019
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9. Detection of the HIV-1 Accessory Proteins Nef and Vpu by Flow Cytometry Represents a New Tool to Study Their Functional Interplay within a Single Infected CD4+ T Cell.

Author

Prévost, Jérémie, Richard, Jonathan, Gasser, Romain, Medjahed, Halima, Kirchhoff, Frank, Hahn, Beatrice H., Kappes, John C., Ochsenbauer, Christina, Duerr, Ralf, and Finzi, Andrés

Subjects
*NEGATIVE regulatory factor, *ANTIBODY-dependent cell cytotoxicity, *T cells, *FLOW cytometry, *VIRAL proteins, *HIV
Abstract

The HIV-1 Nef and Vpu accessory proteins are known to protect infected cells from antibody-dependent cellular cytotoxicity (ADCC) responses by limiting exposure of CD4-induced (CD4+) envelope (Env) epitopes at the cell surface. Although both proteins target the host receptor CD4 for degradation, the extent of their functional redundancy is unknown. Here, we developed an intracellular staining technique that permits the intracellular detection of both Nef and Vpu in primary CD41 T cells by flow cytometry. Using this method, we show that the combined expression of Nef and Vpu predicts the susceptibility of HIV-1-infected primary CD4+ T cells to ADCC by HIV1 plasma. We also show that Vpu cannot compensate for the absence of Nef, thus providing an explanation for why some infectious molecular clones that carry a LucR reporter gene upstream of Nef render infected cells more susceptible to ADCC responses. Our method thus represents a new tool to dissect the biological activity of Nef and Vpu in the context of other host and viral proteins within single infected CD4+ T cells. [ABSTRACT FROM AUTHOR]

Published
2022
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