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Start Over Author "G, van Santen" Publisher american society for microbiology
4 results on '"G, van Santen"'

1. Genetic Characteristics of Methicillin-Resistant Staphylococcus argenteus Isolates Collected in the Dutch National MRSA Surveillance from 2008 to 2021

Author

Sandra Witteveen, Antoni P. A. Hendrickx, Angela de Haan, Daan W. Notermans, Fabian Landman, Marga G. van Santen-Verheuvel, Sabine C. de Greeff, Ed J. Kuijper, Noortje M. van Maarseveen, Saara Vainio, and Leo M. Schouls

Subjects
MRSArg, SCCmec type IV, SCCmec subtype IVc(2B), resistance genes, enterotoxins, Staphylococcus argenteus, Microbiology, QR1-502
Abstract

ABSTRACT Staphylococcus argenteus is a recently described member of the Staphylococcus aureus complex (SAC) and is associated with human disease. The frequency and intensity of infections caused by S. argenteus are similar to those of Staphylococcus aureus. S. argenteus can harbor antibiotic resistance genes and a variety of virulence factors analogous to methicillin-resistant S. aureus (MRSA). The aim of our study was to analyze a collection of isolates in the Dutch national MRSA surveillance from January 2008 until March 2021 that were nontypeable by multilocus variable-number tandem-repeat analysis (MLVA). Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-ToF MS) was used for identifying the S. argenteus isolates, and whole-genome sequencing and SeqSphere were used to generate an in-house whole-genome multilocus sequence typing (wgMLST) scheme for typing the isolates. Furthermore, the presence of antibiotic resistance genes, replicons, and virulence genes was determined. Of 52,467 isolates submitted as MRSA from January 2008 until March 2021, 64 isolates (0.12%) were nontypeable with MLVA, and 54 of them were identified with mass spectrometry (MALDI-ToF MS) as S. argenteus. It appeared in retrospect that the first methicillin-resistant S. argenteus (MRSArg) was already submitted in 2008. An in-house-developed S. argenteus wgMLST scheme revealed that S. argenteus isolates clustered in 5 genomic groups which were characterized by distinct MLST types, resistomes, plasmid replicon families, and virulence factors. All but one isolate carried the staphylococcal chromosomal cassette mec (SCCmec) type IV harboring the methicillin resistance gene mecA and represent MRSArg. Most of the isolates with SCCmec subtype IVc(2B) had a trimethoprim resistance gene, dfrG, and harbored a blaZ-carrying plasmid, and most MRSArg isolates have the immune-modulating genes scn and sak. Nine of the 47 isolates carried enterotoxin-encoding genes seg, sei, sem, seo, and seu, which might be able to cause food poisoning. In some persons there was long-term persistence of MRSArg, and there were several genetically related MRSArg isolates in people living in close proximity, suggesting direct human-human transmission. IMPORTANCE We show that MRSArg has been circulating in the Netherlands since at least 2008. Although MRSArg is distinct from MRSA, it has a comparable population structure and carries similar resistance and virulence genes. The Dutch national MRSA surveillance has been expanded to include other methicillin-resistant members of the S. aureus complex, such as S. argenteus and Staphylococcus schweitzeri.

Published
2022
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2. Multiple Cases of Familial Transmission of Community-Acquired Methicillin-Resistant Staphylococcus aureus

Author

Max Heck, Gerlinde N. Pluister, M. G. van Santen-Verheuvel, A J de Neeling, E. Spalburg, W J B Wannet, Bart A. Eijkelkamp, and X. W. Huijsdens

Subjects
Adult, Male, Microbiology (medical), Staphylococcus aureus, medicine.medical_specialty, Familial transmission, medicine.disease_cause, Microbiology, medicine, Humans, Child, Aged, Netherlands, Family Health, business.industry, Public health, Infant, Bacteriology, biochemical phenomena, metabolism, and nutrition, Middle Aged, Staphylococcal Infections, bacterial infections and mycoses, Methicillin-resistant Staphylococcus aureus, Community-Acquired Infections, Child, Preschool, Female, Methicillin Resistance, business
Abstract

The worldwide emergence of community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) can have severe public health implications. Familial transmissions of CA-MRSA in The Netherlands were investigated. Among the families studied, two clusters of CA-MRSA could be identified. This report demonstrates that family members can serve as reservoirs of CA-MRSA which may become a serious problem in containing the spread of MRSA.

Published
2006
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3. Different Levels of Genetic Homogeneity in Vancomycin-Resistant and -Susceptible Enterococcus faecium Isolates from Different Human and Animal Sources Analyzed by Amplified-Fragment Length Polymorphism

Author

N. London, Rob J. L. Willems, Nienke Bruinsma, Christel Driessen, Anthony E. van den Bogaard, Ellen E. Stobberingh, and Marga G van Santen-Verheuvel

Subjects
Genotype, Swine, Enterococcus faecium, Genetic relationship, Biology, Poultry, Microbiology, Feces, Mechanisms of Resistance, Vancomycin, Animals, Humans, Pharmacology (medical), Gram-Positive Bacterial Infections, Antibacterial agent, Pharmacology, Genetic diversity, Vancomycin Resistance, biology.organism_classification, Anti-Bacterial Agents, Infectious Diseases, Phenotype, Amplified fragment length polymorphism, Restriction fragment length polymorphism, Algorithms, Polymorphism, Restriction Fragment Length
Abstract

The genetic relationship among fecal vancomycin-resistant Enterococcus faecium (VREF) and vancomycin-susceptible E. faecium (VSEF) isolates ( n = 178) from the same populations of pigs, human healthy volunteers, and hospitalized patients (from The Netherlands) and chickens (from The Netherlands and Greece) was studied by amplified-fragment length polymorphism (AFLP). The majority of VREF isolates from pigs, healthy volunteers, and hospitalized patients grouped together (genetic similarity, ≥65%). In a previous AFLP study by our group the VREF isolates from hospitalized patients grouped separately, most likely because these were clinical and not fecal isolates as in the present study. Furthermore, VSEF isolates from humans and pigs were found much more genetically diverse than VREF isolates, whereas VREF and VSEF isolates from chickens clustered together in a separate genogroup (genetic similarity, ≥65%), a pattern clearly distinct from the patterns for human and pig isolates. The present study suggests that pigs are a more important source of VREF for humans than chickens and that human- and pig-derived VSEF isolates seem much more heterogeneous than VREF isolates.

Published
2002

4. Antimicrobial Resistance of Neisseria gonorrhoeae and Emerging Ciprofloxacin Resistance in The Netherlands, 1991 to 1998

Author

Albert J. de Neeling, Rob J. L. Willems, Joke Spaargaren, and Marga G van Santen-Verheuvel

Subjects
DNA Topoisomerase IV, Tetracycline, medicine.medical_treatment, Microbial Sensitivity Tests, Drug resistance, medicine.disease_cause, beta-Lactamases, Microbiology, Antibiotic resistance, Anti-Infective Agents, Ciprofloxacin, medicine, Humans, Pharmacology (medical), Netherlands, Antibacterial agent, Pharmacology, biology, Drug Resistance, Microbial, biochemical phenomena, metabolism, and nutrition, bacterial infections and mycoses, biology.organism_classification, Virology, Neisseria gonorrhoeae, DNA Topoisomerases, Type II, Infectious Diseases, Susceptibility, DNA Gyrase, Beta-lactamase, Neisseriaceae, medicine.drug
Abstract

Surveillance of antibiotic resistance in Neisseria gonorrhoeae showed a decrease in the percentage of β-lactamase-producing isolates but an increase in intermediately penicillin-resistant strains and strains resistant to a high level of tetracycline. MICs for the ciprofloxacin-resistant isolates that emerged increased, and these isolates had mutations in gyrA and parC similar to those observed in the Far East.

Published
2000
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