1. Escherichia coli cytotoxic necrotizing factor 1 blocks cell cycle G2/M transition in uroepithelial cells.
- Author
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Falzano L, Filippini P, Travaglione S, Miraglia AG, Fabbri A, and Fiorentini C
- Subjects
- Bacterial Toxins, Cell Line, Tumor, Cyclin B metabolism, Cyclin B1, Escherichia coli pathogenicity, Humans, Urothelium microbiology, rho GTP-Binding Proteins physiology, Cell Division physiology, Cytotoxins physiology, Escherichia coli physiology, Escherichia coli Proteins physiology, G2 Phase physiology, Growth Inhibitors physiology, Urinary Bladder cytology, Urinary Bladder microbiology
- Abstract
Evidence is accumulating that a growing number of bacterial toxins act by modulating the eukaryotic cell cycle machinery. In this context, we provide evidence that a protein toxin named cytotoxic necrotizing factor 1 (CNF1) from uropathogenic Escherichia coli is able to block cell cycle G(2)/M transition in the uroepithelial cell line T24. CNF1 permanently activates the small GTP-binding proteins of the Rho family that, beside controlling the actin cytoskeleton organization, also play a pivotal role in a large number of other cellular processes, including cell cycle regulation. The results reported here show that CNF1 is able to induce the accumulation of cells in the G(2)/M phase by sequestering cyclin B1 in the cytoplasm and down-regulating its expression. The possible role played by the Rho GTPases in the toxin-induced cell cycle deregulation has been investigated and discussed. The activity of CNF1 on cell cycle progression can offer a novel view of E. coli pathogenicity.
- Published
- 2006
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