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1. Serotype analysis of Streptococcus pneumoniae in lung and nasopharyngeal aspirates from children in the Gambia by MassTag PCR.

Author

Tokarz R, Briese T, Morris G, Ideh R, Chimah O, Ebruke B, Desai A, Haq S, Sameroff S, Howie SR, and Lipkin WI

Subjects
Child, Preschool, Gambia epidemiology, Humans, Infant, Pneumonia, Pneumococcal microbiology, Streptococcus pneumoniae isolation & purification, Lung microbiology, Molecular Typing, Nasopharynx microbiology, Pneumonia, Pneumococcal epidemiology, Polymerase Chain Reaction methods, Serotyping, Streptococcus pneumoniae classification
Abstract

Streptococcus pneumoniae strains comprise >90 serotypes. Here we describe establishment of a MassTag PCR assay designed to serotype S. pneumoniae and demonstrate its utility in tests using 31 paired lung aspirate and nasopharyngeal aspirate samples from children with pneumonia in the Gambia. Serotypes 1, 5, and 14 in were implicated in 90% of lung infections. With 5 exceptions, serotypes found in lung aspirates were also found in nasopharyngeal aspirates.

Published
2013
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