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The standardized disk diffusion test, in which a 10-micrograms enoxacin disk is used, was performed and microbroth dilution MICs were determined to establish individual test control values with Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, and S. aureus ATCC 29213. In addition, regression analysis correlating inhibitory zone diameter with MICs for approximately 400 gram-negative clinical isolates was performed. Based on linear regression and error rate-bounded analyses, criteria for the category calls of isolates are proposed.

Enoxacin (CI-919) was evaluated for activity against 120 multiply resistant clinical isolates of the family Enterobacteriaceae and Pseudomonas aeruginosa. The MIC of enoxacin for 95% of the strains tested was less than or equal to 2 micrograms/ml. Approximately 90% of the isolates were susceptible to enoxacin, norfloxacin, ceftazidime, moxalactam, cefotaxime, cefoperazone, and amikacin. Marked resistance to the other seven antimicrobial agents tested was observed.

Comparisons of the antimicrobial susceptibility patterns of clinical isolates of the family Enterobacteriaceae and Pseudomonas aeruginosa were made by using Kirby-Bauer disk diffusion, the MicroMedia system broth microdilution, and the AutoMicrobic system. The Kirby-Bauer system compared favorably with the MicroMedia system (95% essential correlation with 90% complete agreement). The AutoMicrobic system also compared favorably with both the Kirby-Bauer system (93% essential correlation with 87% complete agreement) and the MicroMedia system (97% essential correlation with 85% complete agreement). These data indicate that the AutoMicrobic system results are comparable to those of the MicroMedia and the Kirby-Bauer systems. Furthermore, the AutoMicrobic system offers a more rapid system (6 to 10 h), than the traditional systems, without sacrificing accuracy (16 to 24 h).

An enzyme immunoassay (Gonozyme; Abbott Laboratories, North Chicago, Ill.) for detection of Neisseria gonorrhoeae antigens was used to screen 184 urethral or uncentrifuged first-voided urine or both specimens from males and 78 cervical specimens. When compared with culture, the sensitivity and specificity of Gonozyme for cervical and urethral specimens were comparable to those in published reports. The sensitivity and specificity for urine specimens were 91.6 and 97.9%, respectively.

Transformation of a Neisseria gonorrhoeae auxotroph (uracil and arginine deficient) to prototrophy was attempted with wild-type deoxyribonucleic acid from 71 random clinical N. gonorrhoeae cultures. Of these 71 cultures, 97.1% transformed the nutritionally deficient mutant to prototrophy. The procedure was reliable and economical and offered several distinct advantages over other methods used for the confirmation of N. gonorrhoeae.