49 results on '"BARTONELLA quintana"'
Search Results
2. Laboratory Diagnosis of 37 Cases of Bartonella Endocarditis Based on Enzyme Immunoassay and Real-Time PCR
- Author
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Ora Halutz, Merav Graidy-Varon, Ariel Velan, Eugene Katchman, Moshe Ephros, Adi Treves, Michal Rasis, Inbal Binsky Ehrenreich, Noam Maisler, Cecilia Leibovitch, Yasmin Maor, Lev Shapira, and Michael Giladi
- Subjects
0301 basic medicine ,Microbiology (medical) ,Bartonella ,030231 tropical medicine ,030106 microbiology ,Real-Time Polymerase Chain Reaction ,Serology ,Immunoenzyme Techniques ,03 medical and health sciences ,0302 clinical medicine ,Bartonella quintana ,Bartonella Infections ,Humans ,Medicine ,Endocarditis ,Serologic Tests ,Bartonella henselae ,biology ,medicine.diagnostic_test ,business.industry ,Bacteriology ,Brucellosis ,bacterial infections and mycoses ,medicine.disease ,biology.organism_classification ,Coxiella burnetii ,Antibodies, Bacterial ,Virology ,Immunoassay ,bacteria ,business - Abstract
Bartonella spp., mostly Bartonella quintana and B. henselae, are a common cause of culture-negative endocarditis. Serology using immunofluorescence assay (IFA) and PCR performed on cardiac tissues are the mainstays of diagnosis. We developed an enzyme immunoassay (EIA) and a novel multiplex real-time PCR assay, utilizing Bartonella genus-specific, B. henselae-specific, and B. quintana-specific SimpleProbe probes, for diagnosis of Bartonella endocarditis. We aimed to evaluate the performance of these assays. Thirty-seven patients with definite endocarditis, 18 with B. henselae, 18 with B. quintana, and 1 with B. koehlerae, were studied. Diagnosis was confirmed by conventional PCR and DNA sequencing of surgical cardiac specimens. Similar to the case with IFA, anti-Bartonella IgG titers of ≥1:800 were found in 94% of patients by EIA; cross-reactivity between B. henselae and B. quintana precluded species-specific serodiagnosis, and frequent (41%) but low-titer cross-reactivity between Coxiella burnetii antibodies and B. henselae antigen was found in patients with Q fever endocarditis. Low-titer (1:100) cross-reactivity was uncommonly found also in patients with brucellosis and culture-positive endocarditis, particularly Enterococcus faecalis endocarditis. Real-time PCR performed on explanted heart valves/vegetations was in complete agreement with results of sequence-based diagnosis with characteristic melting curves. The genus-specific probe identified five additional endocarditis-associated Bartonella spp. at the genus level. In conclusion, EIA coupled with a novel real-time PCR assay can play an important role in Bartonella endocarditis diagnosis and expand the diagnostic arsenal at the disposal of the clinical microbiologist. Since serology remains a major diagnostic tool, recognizing its pitfalls is essential to avoid incorrect diagnosis.
- Published
- 2021
3. Bartonella quintana Aortitis in a Man with AIDS, Diagnosed by Needle Biopsy and 16S rRNA Gene Amplification
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Gina M. Borgo, Jane E. Koehler, Brad T. Cookson, Anne F Luetkemeyer, Michael A. Ohliger, Sulggi A. Lee, Miles Conrad, Sara K. Plett, Dhruba J. Sengupta, and Onderdonk, AB
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Male ,Pathology ,Rifabutin ,Biopsy ,Case Reports ,Medical and Health Sciences ,Bartonella quintana ,hemic and lymphatic diseases ,RNA, Ribosomal, 16S ,Needle ,Cluster Analysis ,rRNA ,Tomography ,Phylogeny ,Doxycycline ,Microscopy ,biology ,medicine.diagnostic_test ,Histocytochemistry ,Biopsy, Needle ,Pain Research ,Bacterial ,Middle Aged ,Biological Sciences ,Antibodies, Bacterial ,Trench Fever ,Trench fever ,Anti-Bacterial Agents ,X-Ray Computed ,Treatment Outcome ,Infectious Diseases ,Sequence Analysis ,Bartonella Infection ,medicine.drug ,DNA, Bacterial ,Microbiology (medical) ,Bartonella ,16S ,medicine.medical_specialty ,Molecular Sequence Data ,DNA, Ribosomal ,Microbiology ,Antibodies ,medicine ,Humans ,Aortitis ,Ribosomal ,Acquired Immunodeficiency Syndrome ,Agricultural and Veterinary Sciences ,Genes, rRNA ,Sequence Analysis, DNA ,DNA ,bacterial infections and mycoses ,biology.organism_classification ,medicine.disease ,Vector-Borne Diseases ,Genes ,bacteria ,RNA ,Tomography, X-Ray Computed - Abstract
A man with newly diagnosed AIDS presented with months of back pain and fever. Computed tomography (CT) results demonstrated aortitis with periaortic tissue thickening. DNA amplification of biopsy tissue revealed Bartonella quintana , and Bartonella serologies were subsequently noted to be positive. The patient improved with prolonged doxycycline and rifabutin treatment. This case illustrates how molecular techniques are increasingly important in diagnosing Bartonella infections.
- Published
- 2015
4. Laboratory Diagnosis of 37 Cases of Bartonella Endocarditis Based on Enzyme Immunoassay and Real-Time PCR.
- Author
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Shapira L, Rasis M, Binsky Ehrenreich I, Maor Y, Katchman EA, Treves A, Velan A, Halutz O, Graidy-Varon M, Leibovitch C, Maisler N, Ephros M, and Giladi M
- Subjects
- Antibodies, Bacterial, Humans, Immunoenzyme Techniques, Real-Time Polymerase Chain Reaction, Serologic Tests, Bartonella genetics, Bartonella Infections diagnosis, Bartonella henselae genetics, Bartonella quintana genetics, Endocarditis
- Abstract
Bartonella spp., mostly Bartonella quintana and B. henselae , are a common cause of culture-negative endocarditis. Serology using immunofluorescence assay (IFA) and PCR performed on cardiac tissues are the mainstays of diagnosis. We developed an enzyme immunoassay (EIA) and a novel multiplex real-time PCR assay, utilizing Bartonella genus-specific, B. henselae -specific, and B. quintana -specific SimpleProbe probes, for diagnosis of Bartonella endocarditis. We aimed to evaluate the performance of these assays. Thirty-seven patients with definite endocarditis, 18 with B. henselae , 18 with B. quintana , and 1 with B. koehlerae , were studied. Diagnosis was confirmed by conventional PCR and DNA sequencing of surgical cardiac specimens. Similar to the case with IFA, anti- Bartonella IgG titers of ≥1:800 were found in 94% of patients by EIA; cross-reactivity between B. henselae and B. quintana precluded species-specific serodiagnosis, and frequent (41%) but low-titer cross-reactivity between Coxiella burnetii antibodies and B. henselae antigen was found in patients with Q fever endocarditis. Low-titer (1:100) cross-reactivity was uncommonly found also in patients with brucellosis and culture-positive endocarditis, particularly Enterococcus faecalis endocarditis. Real-time PCR performed on explanted heart valves/vegetations was in complete agreement with results of sequence-based diagnosis with characteristic melting curves. The genus-specific probe identified five additional endocarditis-associated Bartonella spp. at the genus level. In conclusion, EIA coupled with a novel real-time PCR assay can play an important role in Bartonella endocarditis diagnosis and expand the diagnostic arsenal at the disposal of the clinical microbiologist. Since serology remains a major diagnostic tool, recognizing its pitfalls is essential to avoid incorrect diagnosis., (Copyright © 2021 American Society for Microbiology.)
- Published
- 2021
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5. 'Candidatus Mycoplasma haemomacaque' and Bartonella quintana Bacteremia in Cynomolgus Monkeys
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Nandhakumar Balakrishnan, Patricia E. Mascarelli, Lila Ramaiah, Catherine M. Kelly, Ricardo G. Maggi, Michael W. Leach, Edward B. Breitschwerdt, and Cynthia M. Rohde
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Microbiology (medical) ,RNase P ,Molecular Sequence Data ,Bacteremia ,Sequence alignment ,medicine.disease_cause ,Ribonuclease P ,Microbiology ,Mycoplasma ,Bartonella quintana ,Phylogenetics ,RNA, Ribosomal, 16S ,medicine ,Animals ,Mycoplasma Infections ,Phylogeny ,Base Sequence ,biology ,Monkey Diseases ,Nucleic acid sequence ,Bacteriology ,Sequence Analysis, DNA ,bacterial infections and mycoses ,biology.organism_classification ,16S ribosomal RNA ,medicine.disease ,Virology ,Trench Fever ,Trench fever ,Bacterial Typing Techniques ,Macaca fascicularis ,bacteria ,Sequence Alignment - Abstract
Here, we report latent infections with Bartonella quintana and a hemotropic Mycoplasma sp. in a research colony of cynomolgus monkeys ( Macaca fascicularis ). Sequence alignments, evolutionary analysis, and signature nucleotide sequence motifs of the hemotropic Mycoplasma 16S rRNA and RNase P genes indicate the presence of a novel organism.
- Published
- 2013
6. Hemin-Binding Proteins as Potent Markers for Serological Diagnosis of Infections with Bartonella quintana
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Mayumi Matsuoka, Keigo Shibayama, Mutsuo Kobayashi, Tsuguo Sasaki, Yuko Sasaki, Toshinori Sasaki, Kyoko Sawabe, Yoshichika Arakawa, and Naomi Seki
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Hemeproteins ,Microbiology (medical) ,Antigenicity ,Clinical Biochemistry ,Immunology ,Enzyme-Linked Immunosorbent Assay ,Immunofluorescence ,Serology ,Heme-Binding Proteins ,Antigen ,Bartonella quintana ,Diagnostic Laboratory Immunology ,medicine ,Humans ,Immunology and Allergy ,Serologic Tests ,Sequence Deletion ,Antigens, Bacterial ,biology ,medicine.diagnostic_test ,bacterial infections and mycoses ,biology.organism_classification ,Antibodies, Bacterial ,Virology ,Recombinant Proteins ,Trench Fever ,Epitope mapping ,Immunoglobulin G ,biology.protein ,bacteria ,Antibody ,Carrier Proteins ,Biomarkers ,Epitope Mapping ,Bartonella Infection - Abstract
It is difficult to distinguish infections with different Bartonella species using commercially available immunofluorescence (indirect immunofluorescent antibody [IFA]) assay kits. To identify appropriate proteins for serodiagnosis of Bartonella quintana infections, we investigated the antigenicity of B. quintana proteins using sera from homeless people with high B. quintana IgG titers in IFA assay. These sera reacted strongly to an outer membrane protein, hemin-binding protein D (HbpD). Further, serum from an endocarditis patient infected with B. quintana reacted to HbpB and HbpD. To locate the antigenic sites within the proteins, we generated deletion mutants of HbpB and HbpD. Amino acid residues 89 to 220 of HbpB and 151 to 200 of HbpD were identified as the minimum regions required for recognition by these sera. Several oligopeptides comprising parts of the minimum regions of HbpB and HbpD were synthesized, and their immunoreactivity with the above-mentioned sera was tested by enzyme-linked immunosorbent assay (ELISA). Serum from the endocarditis patient reacted similarly to synthetic peptides HbpB2 (amino acid residues 144 to 173 of HbpB) and HbpD3 (151 to 200 residues of HbpD), while sera from the other subjects reacted to HbpD3. These results indicate that synthetic peptides HbpB2 and HbpD3 might be suitable for developing serological tools for differential diagnosis of B. quintana infections from other Bartonella infections.
- Published
- 2013
7. Trimeric Autotransporter Adhesin-Dependent Adherence of Bartonella henselae, Bartonella quintana, and Yersinia enterocolitica to Matrix Components and Endothelial Cells under Static and Dynamic Flow Conditions
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Volkhard A. J. Kempf, Dirk Linke, Tanja Riess, Andrea I. Schäfer, Johannes A. Eble, Patrick O. Kaiser, Heinz Schwarz, and Niklas F. Müller
- Subjects
Bartonella ,Umbilical Veins ,Virulence Factors ,Immunology ,Fluorescent Antibody Technique ,Yersinia ,Microbiology ,Bacterial Adhesion ,Cell-Matrix Junctions ,Microscopy, Electron, Transmission ,Bartonella quintana ,parasitic diseases ,Humans ,Trimeric autotransporter adhesin ,Adhesins, Bacterial ,Yersinia enterocolitica ,Cells, Cultured ,Bartonella henselae ,biology ,Endothelial Cells ,biology.organism_classification ,Molecular Pathogenesis ,Extracellular Matrix ,Bacterial adhesin ,Infectious Diseases ,Host-Pathogen Interactions ,bacteria ,Parasitology ,Bacterial outer membrane ,Bacterial Outer Membrane Proteins - Abstract
Trimeric autotransporter adhesins (TAAs) are important virulence factors of Gram-negative bacteria responsible for adherence to extracellular matrix (ECM) and host cells. Here, we analyzed three different TAAs ( Bartonella adhesin A [BadA] of Bartonella henselae , variably expressed outer membrane proteins [Vomps] of Bartonella quintana , and Yersinia adhesin A [YadA] of Yersinia enterocolitica ) for mediating bacterial adherence to ECM and endothelial cells. Using static (cell culture vials) and dynamic (capillary flow chambers) experimental settings, adherence of wild-type bacteria and of the respective TAA-negative strains was analyzed. Under static conditions, ECM adherence of B. henselae , B. quintana , and Y. enterocolitica was strongly dependent on the expression of their particular TAAs. YadA of Y. enterocolitica did not mediate bacterial binding to plasma or cellular fibronectin under either static or dynamic conditions. TAA-dependent host cell adherence appeared more significant under dynamic conditions although the total number of bound bacteria was diminished compared to the number under static conditions. Dynamic models expand the methodology to perform bacterial adherence experiments under more realistic, bloodstream-like conditions and allow dissection of the biological role of TAAs in ECM and host cell adherence under static and dynamic conditions.
- Published
- 2011
8. Duplex PCR Assay Simultaneously Detecting and Differentiating Bartonella quintana , B . henselae , and Coxiella burnetii in Surgical Heart Valve Specimens
- Author
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Yi-Wei Tang
- Subjects
Microbiology (medical) ,Bartonella henselae ,biology ,Q fever ,biology.organism_classification ,Coxiella burnetii ,medicine.disease ,Virology ,Rickettsiaceae ,Trench fever ,law.invention ,Microbiology ,law ,medicine ,Bartonella quintana ,Rickettsiales ,Polymerase chain reaction - Abstract
A duplex PCR (dPCR) assay was developed to simultaneously detect and differentiate Bartonella quintana , Bartonella henselae , and Coxiella burnetii from surgical heart valve tissue specimens with an analytic sensitivity of 10 copies/reaction. Among 17 specimens collected from patients with a clinical diagnosis of culture-negative endocarditis, 2, 4, and 2 were positive for B . quintana , B . henselae , and C . burnetii , respectively, by the dPCR assay, which matched the results obtained by universal bacterial 16S rRNA gene amplification and sequencing.
- Published
- 2009
9. Proteomic and Immunoblot Analyses of Bartonella quintana Total Membrane Proteins Identify Antigens Recognized by Sera from Infected Patients
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Jane E. Koehler, Helen L. Gerns, Jenni K. Boonjakuakul, Linda D. Hicks, Michael F. Minnick, Yu Ting Chen, Steven C. Hall, and Scott E. Dixon
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Proteome ,Immunoblotting ,Immunology ,Virulence ,Peptide Mapping ,Microbiology ,Bacterial Proteins ,Antigen ,Peptide mass fingerprinting ,Bartonella quintana ,Humans ,Electrophoresis, Gel, Two-Dimensional ,Antigens, Bacterial ,biology ,Immune Sera ,Membrane Proteins ,Bacterial Infections ,bacterial infections and mycoses ,biology.organism_classification ,Antibodies, Bacterial ,Trench Fever ,Bacterial adhesin ,Infectious Diseases ,Membrane protein ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,bacteria ,Parasitology ,Bacterial outer membrane - Abstract
Bartonella quintana is a fastidious, gram-negative, rod-shaped bacterium that causes prolonged bacteremia in immunocompetent humans and severe infections in immunocompromised individuals. We sought to define the outer membrane subproteome of B. quintana in order to obtain insight into the biology and pathogenesis of this emerging pathogen and to identify the predominant B. quintana antigens targeted by the human immune system during infection. We isolated the total membrane proteins of B. quintana and identified 60 proteins by two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and peptide mass fingerprinting. Using the newly constructed proteome map, we then utilized two-dimensional immunoblotting with sera from 21 B. quintana -infected patients to identify 24 consistently recognized, immunoreactive B. quintana antigens that have potential relevance for pathogenesis and diagnosis. Among the outer membrane proteins, the v ariably expressed o uter m embrane p rotein adhesins (VompA and VompB), peptidyl-prolyl cis-trans -isomerase (PpI), and h emin- b inding p rotein E (HbpE) were recognized most frequently by sera from patients, which is consistent with surface expression of these virulence factors during human infection.
- Published
- 2007
10. Bartonella quintana Variably Expressed Outer Membrane Proteins Mediate Vascular Endothelial Growth Factor Secretion but Not Host Cell Adherence
- Author
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Tanja Riess, Martin Schaller, Volkhard A. J. Kempf, Sandra Klumpp, Dirk Linke, Berit Schulte, and Ingo B. Autenrieth
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medicine.medical_treatment ,Blotting, Western ,Molecular Sequence Data ,Immunology ,Fluorescent Antibody Technique ,Biology ,Immunofluorescence ,Microbiology ,chemistry.chemical_compound ,Bartonella quintana ,Cell Adhesion ,medicine ,Humans ,Secretion ,Amino Acid Sequence ,Neovascularization, Pathologic ,medicine.diagnostic_test ,Vascular Endothelial Growth Factors ,Macrophages ,Growth factor ,Epithelial Cells ,bacterial infections and mycoses ,biology.organism_classification ,Bacillary angiomatosis ,medicine.disease ,Molecular Pathogenesis ,Trench Fever ,Fibronectins ,Protein Structure, Tertiary ,Vascular endothelial growth factor ,Infectious Diseases ,chemistry ,Membrane protein ,bacteria ,Parasitology ,Bacterial outer membrane ,Bacterial Outer Membrane Proteins ,HeLa Cells - Abstract
Bartonella quintana causes trench fever, endocarditis, and the vasculoproliferative disorders bacillary angiomatosis and peliosis hepatis in humans. Little is known about the interaction of this pathogen with host cells. We attempted to elucidate the interaction of B. quintana with human macrophages (THP-1) and epithelial cells (HeLa 229). Remarkably, only B. quintana strain JK-31 induced secretion of vascular endothelial growth factor (VEGF) from THP-1 and HeLa 229 cells upon infection similar to the secretion induced by B. henselae Marseille, whereas other strains ( B. quintana 2-D70, B. quintana Toulouse, and B. quintana Munich) did not induce such secretion. Immunofluorescence testing and electron microscopy revealed that the B. quintana strains unable to induce VEGF secretion did not express the variable outer membrane proteins (Vomps) on their surfaces. Surprisingly, the increase in VEGF secretion mediated by B. quintana JK-31 was not paralleled by elevated host cell adherence rates compared with the rates for Vomp-negative B. quintana strains. Our results suggest that the Vomps play a leading role in the angiogenic reprogramming of host cells by B. quintana but not in the adherence to host cells.
- Published
- 2006
11. Characterization of the Genome Composition of Bartonella koehlerae by Microarray Comparative Genomic Hybridization Profiling
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Christoph Dehio, Olga Vinnere, Siv G. E. Andersson, Alex Mira, Dirk Repsilber, Michaela Dehio, Hillevi Lindroos, and Kristina Näslund
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DNA, Bacterial ,Bartonella ,Genetics ,Bartonella koehlerae ,Bartonella henselae ,Genomics and Proteomics ,biology ,Gene Expression Profiling ,Restriction Mapping ,Nucleic Acid Hybridization ,Genomics ,biology.organism_classification ,Microbiology ,Genome ,Electrophoresis, Gel, Pulsed-Field ,Bartonella quintana ,Molecular Biology ,Genome size ,Genome, Bacterial ,Polymorphism, Restriction Fragment Length ,Oligonucleotide Array Sequence Analysis ,Comparative genomic hybridization - Abstract
Bartonella henselae is present in a wide range of wild and domestic feline hosts and causes cat-scratch disease and bacillary angiomatosis in humans. We have estimated here the gene content of Bartonella koehlerae , a novel species isolated from cats that was recently identified as an agent of human endocarditis. The investigation was accomplished by comparative genomic hybridization (CGH) to a microarray constructed from the sequenced 1.93-Mb genome of B. henselae . Control hybridizations of labeled DNA from the human pathogen Bartonella quintana with a reduced genome of 1.58 Mb were performed to evaluate the accuracy of the array for genes with known levels of sequence divergence. Genome size estimates of B. koehlerae by pulsed-field gel electrophoresis matched that calculated by the CGH, indicating a genome of 1.7 to 1.8 Mb with few unique genes. As in B. quintana , sequences in the prophage and the genomic islands were reported absent in B. koehlerae . In addition, sequence variability was recorded in the chromosome II-like region, where B. koehlerae showed an intermediate retention pattern of both coding and noncoding sequences. Although most of the genes missing in B. koehlerae are also absent from B. quintana , its phylogenetic placement near B. henselae suggests independent deletion events, indicating that host specificity is not solely attributed to genes in the genomic islands. Rather, the results underscore the instability of the genomic islands even within bacterial populations adapted to the same host-vector system, as in the case of B. henselae and B. koehlerae .
- Published
- 2005
12. The Bartonella vinsonii subsp. arupensis Immunodominant Surface Antigen BrpA Gene, Encoding a 382-Kilodalton Protein Composed of Repetitive Sequences, Is a Member of a Multigene Family Conserved among Bartonella Species
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Travis M. Bellville, Steven L. Sviat, Michael Frace, and Robert D. Gilmore
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Bartonella ,Genetics ,Bartonella vinsonii ,Bartonella henselae ,biology ,Protein family ,Immunology ,biology.organism_classification ,Microbiology ,Conserved sequence ,ComputingMethodologies_PATTERNRECOGNITION ,Infectious Diseases ,Bartonella quintana ,Parasitology ,Gene ,Bartonella Infection - Abstract
Bartonella proteins that elicit an antibody response during an infection are poorly defined; therefore, to characterize antigens recognized by the host, a Bartonella genomic expression library was screened with serum from an infected mouse. This process led to the discovery of a Bartonella vinsonii subsp. arupensis gene encoding a 382-kDa protein, part of a gene family encoding large proteins, each containing multiple regions of repetitive segments. The genes were termed brpA to -C ( b artonella r epeat p rotein) and bore significant similarity to genes encoding the BadA adhesin protein and members of the variably expressed outer membrane protein family of proteins from Bartonella henselae and Bartonella quintana , respectively.
- Published
- 2005
13. Multispacer Typing Technique for Sequence-Based Typing of Bartonella quintana
- Author
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Didier Raoult, Cédric Foucault, B. La Scola, Siv G. E. Andersson, and Hillevi Lindroos
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DNA, Bacterial ,Microbiology (medical) ,Genotype ,Sequence analysis ,Molecular Sequence Data ,Bacteremia ,Biology ,DNA sequencing ,Microbiology ,Bartonella quintana ,DNA, Ribosomal Spacer ,Phthiraptera ,Pulsed-field gel electrophoresis ,medicine ,Animals ,Humans ,Typing ,Genetics ,Bacteriology ,Sequence Analysis, DNA ,bacterial infections and mycoses ,medicine.disease ,biology.organism_classification ,Bacillary angiomatosis ,Heart Valves ,Trench Fever ,Trench fever ,Bacterial Typing Techniques ,Electrophoresis, Gel, Pulsed-Field - Abstract
Bartonella quintana is a worldwide fastidious bacterium of the Alphaproteobacteria responsible for bacillary angiomatosis, trench fever, chronic lymphadenopathy, and culture-negative endocarditis. The recent genome sequencing of a B. quintana isolate allowed us to propose a genome-wide sequence-based typing method. To ensure sequence discrimination based on highly polymorphic areas, we amplified and sequenced 34 spacers in a large collection of B. quintana isolates. Six of these exhibited polymorphisms and allowed the characterization of 4 genotypes. However, the strain variants suggested by the noncoding sequences did not correlate with the results of pulsed-field gel electrophoresis (PFGE), which suggested a higher degree of variability. Modification of the PFGE profile of one isolate after nine subcultures confirmed that rearrangement frequencies are high in this species, making PFGE unreliable for epidemiological purposes. The low extent of sequence heterogeneity in the species suggests a recent emergence of this bacterium as a human pathogen. Direct typing of natural samples allowed the identification of a fifth genotype in the DNA extracted from a human body louse collected in Burundi. We have named the typing technique herein described multispacer typing.
- Published
- 2005
14. Molecular Characterization of the sucB Gene Encoding the Immunogenic Dihydrolipoamide Succinyltransferase Protein of Bartonella vinsonii subsp. berkhoffii and Bartonella quintana
- Author
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Robert D. Gilmore, Kenneth L. Gage, Amber M. Carpio, and Michael Y. Kosoy
- Subjects
Bartonella ,Bartonella vinsonii ,biology ,Immunology ,Cat-scratch disease ,bacterial infections and mycoses ,biology.organism_classification ,medicine.disease ,Microbiology ,Virology ,Trench fever ,Infectious Diseases ,Rickettsia typhi ,medicine ,bacteria ,Bartonella quintana ,Parasitology ,Francisella tularensis ,Bartonella Infection - Abstract
Members of the genus Bartonella have historically been connected with human disease, such as cat scratch disease, trench fever, and Carrion's disease, and recently have been recognized as emerging pathogens causing other clinical manifestations in humans. However, because little is known about the antigens that elicit antibody production in response to Bartonella infections, this project was undertaken to identify and molecularly characterize these immunogens. Immunologic screening of a Bartonella vinsonii subsp. berkhoffii genomic expression library with anti- Bartonella antibodies led to the identification of the sucB gene, which encodes the enzyme dihydrolipoamide succinyltransferase. Antiserum from a mouse experimentally infected with live Bartonella was reactive against recombinant SucB, indicating the mounting of an anti-SucB response following infection. Antigenic cross-reactivity was observed with antiserum against other Bartonella spp. Antibodies against Coxiella burnetti , Francisella tularensis , and Rickettsia typhi also reacted with our recombinant Bartonella SucB. Potential SucB antigenic cross-reactivity presents a challenge to the development of serodiagnostic tests for other intracellular pathogens that cause diseases such as Q fever, rickettsioses, brucelloses, tularemia, and other bartonelloses.
- Published
- 2003
15. Western Immunoblotting for Bartonella Endocarditis
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Didier Raoult and Pierre Houpikian
- Subjects
Male ,Microbiology (medical) ,Bartonella ,Blotting, Western ,Clinical Biochemistry ,Immunology ,Bacteremia ,Cross Reactions ,Serology ,Bartonella Infections ,medicine ,Humans ,Immunology and Allergy ,Endocarditis ,Serologic Tests ,Antigens, Bacterial ,biology ,Cat-Scratch Disease ,Cat-scratch disease ,Endocarditis, Bacterial ,Middle Aged ,bacterial infections and mycoses ,medicine.disease ,biology.organism_classification ,Antibodies, Bacterial ,Virology ,biology.protein ,Bartonella quintana ,Female ,Microbial Immunology ,Antibody ,Bartonella Infection - Abstract
To differentiate infectious endocarditis (IE) from other Bartonella infections and to identify infecting Bartonella bacteria at the species level on a serological basis, we used Western immunoblotting to test sera from 51 patients with Bartonella IE (of which 27 had previously benefited from species identification by molecular techniques), 11 patients with chronic Bartonella quintana bacteremia, and 10 patients with cat scratch disease. Patients with IE were Western blot positive in 49 of 51 cases, and significant cross-reactivity with three heterologous Bartonella antigens was found in 45 of 49 cases. Sera from bacteremic patients did not react with more than one heterologous antigen, and sera from patients with cat scratch disease gave negative results. Sera reacted only with B. henselae in four cases of IE, including one with a positive PCR result for valve tissue. Western blot and cross-adsorption performed on serum samples from patients with IE (the identity of the causative species having been determined by PCR) were demonstrated to identify efficiently the causative species in all cases. When applied to patients diagnosed on the basis of serological tests only, this technique allowed identification of the causative species in 20 of 22 cases. The results were in accordance with epidemiological features. Six reactive bands of B. quintana (of molecular sizes from 10 to 83 kDa) demonstrated significant association with sera from patients with B. quintana endocarditis. Overall, Western blotting and cross-adsorption made it possible to identify the causative species in 49 of 51 (96%) IE cases.
- Published
- 2003
16. Molecular Characterization of First Human Bartonella Strain Isolated in Italy
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Andrea Petrucca, Simonetta Ciarrocchi, L. Ciceroni, Enrico Farnetti, A. Fabio, Bruno B Chomel, Lucio Bonazzi, Alessandra Ciervo, and A. Pinto
- Subjects
Adult ,Male ,Microbiology (medical) ,Bartonella ,Sequence analysis ,HIV Infections ,Polymerase Chain Reaction ,Microbiology ,law.invention ,Bartonella quintana ,law ,RNA, Ribosomal, 16S ,medicine ,Humans ,Polymerase chain reaction ,Strain (chemistry) ,biology ,Fatty Acids ,Bacteriology ,Sequence Analysis, DNA ,Ribosomal RNA ,bacterial infections and mycoses ,Bacillary angiomatosis ,medicine.disease ,biology.organism_classification ,16S ribosomal RNA ,Virology ,Bacterial Typing Techniques ,Electrophoresis, Gel, Pulsed-Field ,Italy ,Angiomatosis, Bacillary ,bacteria ,Polymorphism, Restriction Fragment Length - Abstract
The aim of this study was to characterize a Bartonella strain (BA-1) isolated from a blood culture of an Italian, human immunodeficiency virus-positive patient with bacillary angiomatosis. We analyzed the isolate using molecular biology methods such as whole-cell fatty acid analysis, PCR-restriction fragment length polymorphism analysis, type-specific 16S rRNA PCRs, sequence analysis of the 16S rRNA, pulsed-field gel electrophoresis, and arbitrarily primed PCR. The BA-1 isolate turned out to be a Bartonella quintana strain, similar but not identical to B. quintana Oklahoma, which was used as a control strain.
- Published
- 2001
17. Use of rpoB Gene Analysis for Detection and Identification of Bartonella Species
- Author
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Didier Raoult, Joanny Gouvernet, Michel Drancourt, Patricia Renesto, and Véronique Roux
- Subjects
DNA, Bacterial ,Microbiology (medical) ,Bartonella ,Bartonella tribocorum ,Chlamydiology and Rickettsiology ,Molecular Sequence Data ,Polymerase Chain Reaction ,Bartonella quintana ,Bartonella Infections ,Sequence Homology, Nucleic Acid ,Genotype ,Animals ,Humans ,Genetics ,Bartonella henselae ,Suppuration ,Base Sequence ,biology ,DNA-Directed RNA Polymerases ,biology.organism_classification ,rpoB ,Bartonella elizabethae ,Bartonella grahamii ,Cats ,Lymph Nodes ,Restriction fragment length polymorphism ,Sequence Alignment ,Polymorphism, Restriction Fragment Length ,Bartonella Infection - Abstract
Identification of Bartonella species is of increasing importance as the number of infections in which these bacteria are involved increases. To date, these gram-negative bacilli have been identified by various serological, biochemical, and genotypic methods. However, the development of alternative tools is required, principally to circumvent a major risk of contamination during sample manipulation. The aim of our study was to investigate the possible identification of various Bartonella species by comparison of RNA polymerase beta-subunit gene ( rpoB ) sequences. This approach has previously been shown to be useful for the identification of members of the family Enterobacteriaceae (C. M. Mollet, M. Drancourt, and D. Raoult, Mol. Microbiol. 26:1005–1011, 1997). Following PCR amplification with specific oligonucleotides, a 825-bp region of the rpoB gene was sequenced from 13 distinct Bartonella strains. Analysis of these sequences allowed selection of three restriction enzymes ( Apo I, Alu I, and Afl III) useful for discerning the different strains by PCR-restriction fragment length polymorphism (PCR-RFLP) analysis. To confirm the potential value of such an approach for identification of Bartonella , the rpoB PCR was then applied to 94 clinical samples, and the results obtained were identical to those obtained by our reference PCR method. Twenty-four isolates were also adequately identified by PCR-RFLP analysis. In all cases, our results were in accordance with those of the reference method. Moreover, conserved regions of DNA were chosen as suitable primer targets for PCR amplification of a 439-bp fragment which can be easily sequenced.
- Published
- 2001
18. Hemin-Binding Surface Protein from Bartonella quintana
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Laura S. Smitherman, Michael F. Minnick, James A. Carroll, and Sherry A. Coleman
- Subjects
Immunoelectron microscopy ,Molecular Sequence Data ,Immunology ,medicine.disease_cause ,Microbiology ,chemistry.chemical_compound ,Bartonella quintana ,Escherichia coli ,medicine ,Amino Acid Sequence ,Cloning, Molecular ,Cellular Microbiology: Pathogen-Host Cell Molecular Interactions ,Base Sequence ,biology ,Binding protein ,biology.organism_classification ,Recombinant Proteins ,Molecular Weight ,Infectious Diseases ,chemistry ,Porin ,biology.protein ,Hemin ,Parasitology ,Carrier Proteins ,Bacterial outer membrane ,Protein A - Abstract
Bartonella quintana , the agent of trench fever and a cause of endocarditis and bacillary angiomatosis in humans, has the highest reported in vitro hemin requirement for any bacterium. We determined that eight membrane-associated proteins from B. quintana bind hemin and that a ∼25-kDa protein (HbpA) was the dominant hemin-binding protein. Like many outer membrane proteins, HbpA partitions to the detergent phase of a Triton X-114 extract of the cell and is heat modifiable, displaying an apparent molecular mass shift from approximately 25 to 30 kDa when solubilized at 100°C. Immunoblots of purified outer and inner membranes and immunoelectron microscopy with whole cells show that HbpA is strictly located in the outer membrane and surface exposed, respectively. The N-terminal sequence of mature HbpA was determined and used to clone the HbpA-encoding gene ( hbpA ) from a lambda genomic library. The hbpA gene is 816 bp in length, encoding a predicted immature protein of approximately 29.3 kDa and a mature protein of 27.1 kDa. A Fur box homolog with 53% identity to the Escherichia coli Fur consensus is located upstream of hbpA and may be involved in regulating expression. BLAST searches indicate that the closest homologs to HbpA include the Bartonella henselae phage-associated membrane protein, Pap31 (58.4% identity), and the OMP31 porin from Brucella melitensis (31.7% identity). High-stringency Southern blots indicate that all five pathogenic Bartonella spp. possess hbpA homologs. Recombinant HbpA can bind hemin in vitro; however, it does not confer a hemin-binding phenotype upon E. coli . Intact B. quintana treated with purified anti-HbpA Fab fragments show a significant ( P < 0.004) dose-dependent decrease in hemin binding relative to controls, suggesting that HbpA plays an active role in hemin acquisition and therefore pathogenesis. HbpA is the first potential virulence determinant characterized from B. quintana .
- Published
- 2000
19. Species-Specific Monoclonal Antibodies for Rapid Identification ofBartonella quintana
- Author
-
Zhongxing Liang and Didier Raoult
- Subjects
Microbiology (medical) ,Bartonella ,medicine.drug_class ,Blotting, Western ,Clinical Biochemistry ,Immunology ,Antibiotics ,Fluorescent Antibody Technique ,Bacillus ,Cross Reactions ,Microbiology ,Epitopes ,Species Specificity ,Bartonella quintana ,Gram-Negative Bacteria ,medicine ,Animals ,Immunology and Allergy ,Endocarditis ,biology ,Microchemistry ,Pediculus ,Antibodies, Monoclonal ,bacterial infections and mycoses ,biology.organism_classification ,medicine.disease ,Bacillary angiomatosis ,Virology ,Trench fever ,Immunoglobulin G ,Bacteremia ,bacteria ,Electrophoresis, Polyacrylamide Gel ,Microbial Immunology - Abstract
Bartonella spp. are gram-negative, short-rod bacteria. Presently there are 14 recognized species within the genus Bartonella (2, 3, 9, 11, 12), and of these, four species are currently recognized as human pathogens: B. bacilliformis, B. quintana, B. henselae, and B. elizabethae (6). B. bacilliformis was the earliest species of this genus to be described (23) and is the agent of Carrion's disease. Infections with B. bacilliformis have yet to be reported from outside a very restricted geographic region in the Andes of western South America. B. quintana was first recognized during World War I as the etiological agent of trench fever. Although Vinson and Fuller (28) isolated the organism in 1961, there was little scientific interest in the organism or trench fever for the next 20 years, as they were apparently only very rarely encountered. Recent investigations, however, have led to the reemergence of B. quintana as an organism of medical importance. Bacillary angiomatosis was initially characterized by the appearance of multiple cutaneous lesions, which were assumed to be infectious because these lesions contained bacilli that stained with Warthin-Starry stain (1, 5, 16) and resolved with antibiotic treatment (5). Subsequently the observed bacillus was characterized by PCR and 16S rRNA gene sequencing, which showed it to be a new organism closely related to B. quintana (22), and in 1992 B. quintana was isolated from skin lesions of bacillary angiomatosis patients (14). The organism has also been found to be associated with other, less specific clinical syndromes, such as bacteremia (26), endocarditis (7, 19, 27), chronic lymphadenopathy (20), neurological disorders (29), and chronic bacteremia in homeless patients (4). There is a need, then, for rapid and specific methods to identify B. quintana and differentiate it from other Bartonella species. In this report we describe the characteristics and specificities of seven species-specific monoclonal antibodies (MAbs) that we produced against B. quintana.
- Published
- 2000
20. Semiquantitative Species-Specific Detection of Bartonella henselae and Bartonella quintana by PCR-Enzyme Immunoassay
- Author
-
Anna Sander and Susanne Penno
- Subjects
DNA, Bacterial ,Microbiology (medical) ,Bartonella ,Dot blot ,Polymerase Chain Reaction ,Sensitivity and Specificity ,Microbiology ,law.invention ,Immunoenzyme Techniques ,Species Specificity ,Bartonella quintana ,law ,medicine ,Humans ,Polymerase chain reaction ,Bartonella henselae ,biology ,medicine.diagnostic_test ,Bacteriology ,Cat-scratch disease ,bacterial infections and mycoses ,biology.organism_classification ,medicine.disease ,Bacillary angiomatosis ,Molecular biology ,Immunoassay ,bacteria - Abstract
Bartonella henselae is the main causative agent of cat-scratch disease, and both B. henselae and Bartonella quintana cause angioproliferative disorders such as bacillary angiomatosis. To increase the sensitivity of Bartonella detection by PCR and to improve the species differentiation, we developed a semiquantitative, species-specific PCR-based enzyme immunoassay (EIA). The 16S rRNA gene was selected as the target sequence. Internal nucleotide sequences derived from the amplified 16S rRNA region were used to develop species-specific oligonucleotide probes for B. henselae and B. quintana . Biotin-labeled PCR products were immobilized on streptavidin-coated microtiter plates, hybridized to a digoxigenin-labeled probe, and detected with antidigoxigenin peroxidase conjugate. No cross-hybridization with other Bartonella or non- Bartonella species was observed. This EIA was as sensitive as dot blot hybridization and was 10 times more sensitive than visualization of PCR products on agarose gels. Serial dilutions of B. henselae and B. quintana suspensions demonstrated that an optical density (OD) of approximately 0.200 was equivalent to 5 CFU in the reaction mixture. By comparing the OD of the bacterial dilutions with that obtained from clinical specimens we could determine that the number of CFU in clinical samples ranged from 10 3 to 10 6 CFU/ml. The PCR-EIA developed in the present study is a rapid, sensitive, and simple method for the diagnosis of B. henselae and B. quintana infections.
- Published
- 1999
21. Diagnosis of Rickettsial Diseases Using Samples Dried on Blotting Paper
- Author
-
Didier Raoult and Florence Fenollar
- Subjects
Paper ,Microbiology (medical) ,Serial dilution ,Clinical Biochemistry ,Immunology ,Fluorescent Antibody Technique ,Article ,Serology ,Humans ,Immunology and Allergy ,False Positive Reactions ,Rickettsia ,Blood Specimen Collection ,Venipuncture ,biology ,Microchemistry ,Rickettsia Infections ,Coxiella burnetii ,biology.organism_classification ,Antibodies, Bacterial ,Virology ,Titer ,Bartonella quintana ,Sample collection ,Rickettsia conorii ,Filtration - Abstract
The use of filter paper is an inexpensive and convenient method for collecting, storing, and transporting blood samples for serological studies. In addition, samples occupy little space and can be readily transported without refrigeration. Rickettsial diseases often evolve according to an epidemic mode and are now considered reemerging diseases, especially in developing countries, under conditions where fieldwork could be difficult. The suitability of collecting whole-blood specimens on filter paper discs for rickettsial antibody assay was evaluated. Dried blood specimens from 64 individuals with antibodies to Coxiella burnetii , Bartonella quintana , or Rickettsia conorii were tested for rickettsial antibodies by microimmunofluorescence. Although occasional titers were 1 or 2 dilutions lower than those of tested serum samples, no statistically significant differences were observed. Among patients with negative serology, no false positives were found. This study demonstrated that the recovery of antibodies from finger-stick blood dried on filter paper after elution produces results comparable to those obtained by recovering antibodies from serum. Storing paper samples for 1 month at room temperature or at 4°C did not significantly affect the level of antibodies recovered. This report shows the utility of this sample collection method in developing countries where refrigeration is not possible and venipuncture is problematic.
- Published
- 1999
22. Culture of Bartonella quintana and Bartonella henselae from Human Samples: a 5-Year Experience (1993 to 1998)
- Author
-
Didier Raoult and Bernard La Scola
- Subjects
Microbiology (medical) ,Pathology ,medicine.medical_specialty ,Bacteremia ,Polymerase Chain Reaction ,Sensitivity and Specificity ,Microbiology ,Bartonella quintana ,Animals ,Humans ,Medicine ,Endocarditis ,Blood culture ,Serotyping ,Bartonella henselae ,medicine.diagnostic_test ,biology ,Bartonellosis ,business.industry ,Cat-Scratch Disease ,Bacteriology ,Cat-scratch disease ,Endocarditis, Bacterial ,medicine.disease ,biology.organism_classification ,Bacillary angiomatosis ,Trench Fever ,Bacterial Typing Techniques ,Ill-Housed Persons ,Angiomatosis, Bacillary ,Cats ,France ,business - Abstract
Bartonella quintana and Bartonella henselae are fastidious gram-negative bacteria responsible for bacillary angiomatosis, trench fever, cat scratch disease, and endocarditis. During a 5-year period, we received 2,043 samples for culture of Bartonella sp. We found Bartonella sp. to be the etiologic agent in 38 cases of endocarditis, 78 cases of cat scratch disease, 16 cases of bacteremia in homeless people, and 7 cases of bacillary angiomatosis. We correlated the results of positive cultures with the clinical form of the disease, type of sample, culture procedure, PCR-based genomic detection, and antibody determination. Seventy-two isolates of B. quintana and nine isolates of B. henselae from 43 patients were obtained. Sixty-three of the B. quintana isolates and two of the B. henselae isolates, obtained from patients with no prior antibiotic therapy, were stably subcultured. The sensitivity of culture was low when compared with that of PCR-based detection methods in valves of patients with endocarditis (44 and 81%, respectively), skin biopsy samples of patients with bacillary angiomatosis (43 and 100%, respectively), and lymph nodes of cat scratch disease (13 and 30%, respectively). Serological diagnosis was also more sensitive in cases of endocarditis (97%) and cat scratch disease (90%). Among endocarditis patients, the sensitivity of the shell vial culture assay was 28% when inoculated with blood samples and 44% when inoculated with valvular biopsy samples, and the sensitivity of both was significantly higher than that of culture on agar (5% for blood [ P = 0.045] and 4% for valve biopsy samples [ P < 0.0005]). The most efficient culture procedure was the subculture of blood culture broth into shell vials (sensitivity, 71%). For patients with endocarditis, previous antibiotic therapy significantly affected results of blood culture; no patient who had been administered antibiotics yielded a positive blood culture, whereas 80% of patients with no previous antibiotic therapy yielded positive blood cultures ( P = 0.0006). Previous antibiotic therapy did not, however, prevent isolation of Bartonella sp. from cardiac valves but did prevent the establishment of strains, as none of the 15 isolates from treated patients could be successfully subcultured. For the diagnosis of B. quintana bacteremia in homeless people, the efficiency of systematic subculture of blood culture broth onto agar was higher than that of direct blood plating (respective sensitivities, 98 and 10% [ P < 10 −7 ]). Nevertheless, both procedures are complementary, since when used together their sensitivity reached 100%. All homeless people with positive blood cultures had negative serology. The isolation rate of B. henselae from PCR-positive lymph nodes, in patients with cat scratch disease, was significantly lower than that from valves of endocarditis patients and skin biopsy samples from bacillary angiomatosis patients (13 and 33%, respectively [ P = 0.084]). In cases of bacillary angiomatosis for which an agent was identified to species level, the isolation rate of B. henselae was lower than the isolation rate of B. quintana (28 and 64%, respectively [ P = 0.003]). If culture is to be considered an efficient tool for the diagnosis of several Bartonella -related diseases, methodologies need to be improved, notably for the recovery of B. henselae from lymph nodes of patients with cat scratch disease.
- Published
- 1999
23. Seroprevalence of Antibodies to Bartonella henselae in Patients with Cat Scratch Disease and in Healthy Controls: Evaluation and Comparison of Two Commercial Serological Tests
- Author
-
Karin Oberle, Miriam Posselt, Anna Sander, and Wolfgang Bredt
- Subjects
Adult ,Microbiology (medical) ,Adolescent ,Clinical Biochemistry ,Immunology ,Cross Reactions ,Sensitivity and Specificity ,Article ,Immunoglobulin G ,Serology ,Bartonella quintana ,Germany ,medicine ,Animals ,Humans ,Immunology and Allergy ,Seroprevalence ,Serologic Tests ,Child ,Fluorescent Antibody Technique, Indirect ,Bartonella henselae ,biology ,Cat-Scratch Disease ,Cat-scratch disease ,Middle Aged ,biology.organism_classification ,medicine.disease ,Antibodies, Bacterial ,Virology ,Titer ,Immunoglobulin M ,Case-Control Studies ,Child, Preschool ,Cats ,biology.protein - Abstract
Serologic testing for the presence of antibodies to Bartonella henselae is a widely accepted diagnostic procedure for laboratory confirmation of the diagnosis of cat scratch disease (CSD). In this study a commercially available indirect immunofluorescence assay (IFA) based on B. henselae -infected human larynx carcinoma cells (test A) was evaluated. Sera from 42 patients with CSD (20 confirmed by PCR) and 270 sera from healthy controls (consisting of 63 cat owners, 65 individuals whose last close contact with cats was >6 months previously, and 142 persons who had never been exposed to cats) were investigated for antibodies to B. henselae . All patients with CSD had titers of immunoglobulin G (IgG) to B. henselae of 128 or higher (test A; sensitivity, 100%). Of the 270 controls 189 (70%) were seronegative (titer, B. henselae and Bartonella quintana (test B). The sensitivity and specificity of test B were 93 and 73%, respectively. For patients with CSD the cross-reactivity between B. henselae and B. quintana in this test was 95%. Both systems are highly sensitive but less specific for detection of IgG antibodies to B. henselae in samples from patients with clinically apparent CSD. For detection of IgM antibodies, test A seems to be more sensitive (88%) and more specific (95%) than test B (sensitivity and specificity of 64 and 86%, respectively). The data show that the seroprevalence of antibodies to B. henselae in German individuals is high (30%). Low antibody levels are not sufficient evidence of active or prior infection.
- Published
- 1998
24. Detection and identification of two Bartonella henselae variants in domestic cats in Germany
- Author
-
C Bühler, Wolfgang Bredt, E von Cramm, K. Pelz, and Anna Sander
- Subjects
Male ,Microbiology (medical) ,Bartonella ,Bacteremia ,Polymerase Chain Reaction ,Bartonella clarridgeiae ,Microbiology ,law.invention ,Species Specificity ,Bartonella quintana ,law ,Bartonella Infections ,Germany ,RNA, Ribosomal, 16S ,Animals ,Polymerase chain reaction ,Bartonella henselae ,CATS ,biology ,Fatty Acids ,Genetic Variation ,biology.organism_classification ,Virology ,RNA, Bacterial ,Gram staining ,Cats ,Female ,Bartonella Infection ,Research Article - Abstract
To determine the prevalence of bacteremia caused by Bartonella henselae in domestic cats in the region of Freiburg, Germany, we investigated culture of blood from 100 cats from 89 different households over a 12-month period. B. henselae could be isolated from 13% (13 of 100) of these cats. In eight households with two cats each and in one household with three cats, B. henselae bacteremia was found either in all of the animals or in none of the animals. Positive cultures were more likely to be found for female, young (24 months of age or younger) cats than for male or older cats. Identification of the Bartonella isolates was made by colony morphology, by Gram staining, biochemically by RapID ANA II or Rapid ID 32 A systems, and by whole-cell fatty acid analysis. Differentiation between B. henselae and Bartonella quintana was only possible by 16S rRNA sequencing, enterobacterial repetitive intergenic consensus (ERIC)-PCR and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Genomic fingerprinting of the B. henselae isolates by ERIC-PCR yielded two different patterns based on three distinct bands.
- Published
- 1997
25. Detection of Bartonella quintana by Direct Immunofluorescence Examination of Blood Smears of a Patient with Acute Trench Fever
- Author
-
Cédric Foucault, Jean-Marc Rolain, Didier Raoult, and Philippe Brouqui
- Subjects
Male ,Microbiology (medical) ,Pathology ,medicine.medical_specialty ,Erythrocytes ,Erythroblasts ,Bartonellaceae ,Fluorescent Antibody Technique ,HIV Infections ,Case Reports ,Immunofluorescence ,Bartonella quintana ,hemic and lymphatic diseases ,medicine ,Humans ,Direct fluorescent antibody ,Blood Specimen Collection ,biology ,medicine.diagnostic_test ,Middle Aged ,bacterial infections and mycoses ,medicine.disease ,biology.organism_classification ,Virology ,Trench Fever ,Trench fever ,Rickettsiosis ,Blood smear ,Acute Disease ,Ill-Housed Persons ,bacteria - Abstract
We report a case of Bartonella quintana acute symptomatic infection in a homeless man, presenting as a typical trench fever. B. quintana has been retrieved in erythrocytes in large clusters and in erythroblasts. Direct immunofluorescence of blood smears allows a rapid diagnosis.
- Published
- 2004
26. Bartonella henselae and Bartonella quintana adherence to and entry into cultured human epithelial cells
- Author
-
Stanley Falkow, Lucy S. Tompkins, H J Batterman, J S Loutit, and J A Peek
- Subjects
Immunology ,Biology ,Microbiology ,Bacterial Adhesion ,Epithelium ,Pilus ,Cell–cell interaction ,Bartonella quintana ,Serial passage ,Humans ,Cells, Cultured ,Phase variation ,Bartonella henselae ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,Virology ,Infectious Diseases ,Cell culture ,Fimbriae, Bacterial ,bacteria ,Parasitology ,Bacteria ,Research Article - Abstract
Bartonella henselae expresses pili phenotypically similar to type 4 pili. B. henselae pilus expression undergoes phase variation with multiple passages. Low-passage-number, piliated B. henselae adhered to and invaded HEp-2 cells to a greater extent than did multiply passaged B. henselae with reduced pilus expression. Pili may be a pathogenic determinant for Bartonella species.
- Published
- 1995
27. Inter- and intraspecies identification of Bartonella (Rochalimaea) species
- Author
-
Didier Raoult and Véronique Roux
- Subjects
Microbiology (medical) ,Bartonella ,Genetics ,Bartonella koehlerae ,biology ,Cat-scratch disease ,bacterial infections and mycoses ,medicine.disease ,biology.organism_classification ,Bacillary angiomatosis ,HaeIII ,medicine ,bacteria ,Bartonella quintana ,Restriction fragment length polymorphism ,Bartonella Infection ,Research Article ,medicine.drug - Abstract
Species of the genus Rochalimaea, recently renamed Bartonella, are of a growing medical interest. Bartonella quintana was reported as the cause of trench fever, endocarditis, and bacillary angiomatosis. B. henselae has been implicated in symptoms and infections of human immunodeficiency virus-infected patients, such as fever, endocarditis, and bacillary angiomatosis, and is involved in the etiology of cat scratch disease. Such a wide spectrum of infections makes it necessary to obtain an intraspecies identification tool in order to perform epidemiological studies. B. vinsonii, B. elizabethae, seven isolates of B. quintana, and four isolates of B. henselae were studied by pulsed-field gel electrophoresis (PFGE) after restriction with the infrequently cutting endonucleases NotI, EagI, and SmaI. Specific profiles were obtained for each of the four Bartonella species. Comparison of genomic fingerprints of isolates of the same species showed polymorphism in DNA restriction patterns, and a specific profile was obtained for each isolate. A phylogenetic analysis of the B. quintana isolates was obtained by using the Dice coefficient, UPGMA (unweighted pair-group method of arithmetic averages), and Package Philip programming. Amplification by PCR and subsequent sequencing using an automated laser fluorescent DNA sequencer (Pharmacia) was performed on the intergenic spacer region (ITS) between the 16 and 23S rRNA genes. It was found that each B. henselae isolate had a specific sequence, while the B. quintana isolates fell into only two groups. When endonuclease restriction analysis of the ITS PCR product was done, three enzymes, TaqI, HindIII, and HaeIII, allowed species identification of Bartonella spp. Restriction fragment length polymorphism after PCR amplification of the 16S-23S rRNA gene ITS may be useful for rapid species identification, and PFGE could be an efficient method for isolate identification.
- Published
- 1995
28. High Frequency of Tropheryma whipplei in Culture-Negative Endocarditis
- Author
-
Andreas J. Morguet, Florence Fenollar, René Tandler, Christian Bogdan, Annette Moter, Christoph Loddenkemper, Andreas Jansen, Walter Geissdörfer, Didier Raoult, Verena Moos, and Thomas Schneider
- Subjects
Microbiology (medical) ,DNA, Bacterial ,Male ,Tropheryma ,Eikenella corrodens ,DNA, Ribosomal ,Polymerase Chain Reaction ,Microbiology ,Tropheryma whipplei ,Cohort Studies ,Germany ,RNA, Ribosomal, 16S ,medicine ,Endocarditis ,Humans ,In Situ Hybridization, Fluorescence ,Aged ,Academic Medical Centers ,Bacteriological Techniques ,Microscopy ,biology ,Histocytochemistry ,Incidence ,Kingella kingae ,Bacteriology ,Endocarditis, Bacterial ,Sequence Analysis, DNA ,Middle Aged ,biology.organism_classification ,medicine.disease ,Virology ,Heart Valves ,Immunohistochemistry ,Infective endocarditis ,Bartonella quintana ,Female ,Cardiobacterium hominis ,Actinomycetales Infections - Abstract
“Classical” Whipple's disease (cWD) is caused by Tropheryma whipplei and is characterized by arthropathy, weight loss, and diarrhea. T. whipplei infectious endocarditis (TWIE) is rarely reported, either in the context of cWD or as isolated TWIE without signs of systemic infection. The frequency of TWIE is unknown, and systematic studies are lacking. Here, we performed an observational cohort study on the incidence of T. whipplei infection in explanted heart valves in two German university centers. Cardiac valves from 1,135 patients were analyzed for bacterial infection using conventional culture techniques, PCR amplification of the bacterial 16S rRNA gene, and subsequent sequencing. T. whipplei -positive heart valves were confirmed by specific PCR, fluorescence in situ hybridization, immunohistochemistry, histological examination, and culture for T. whipplei . Bacterial endocarditis was diagnosed in 255 patients, with streptococci, staphylococci, and enterococci being the main pathogens. T. whipplei was the fourth most frequent pathogen, found in 16 (6.3%) cases, and clearly outnumbered Bartonella quintana , Coxiella burnetii , and members of the HACEK group ( Haemophilus species, Actinobacillus actinomycetemcomitans , Cardiobacterium hominis , Eikenella corrodens , and Kingella kingae ). In this cohort, T. whipplei was the most commonly found pathogen associated with culture-negative infective endocarditis.
- Published
- 2012
29. Detection of Bartonella (Rochalimaea) quintana by routine acridine orange staining of broth blood cultures
- Author
-
D J Nowowiejski, Ghassan M. Matar, B Swaminathan, Molly J. Dougherty, Marie B. Coyle, Ann M. Larson, and D F Welch
- Subjects
Adult ,Microbiology (medical) ,Bartonella ,Time Factors ,Bacteremia ,Microbiology ,Chocolate agar ,chemistry.chemical_compound ,Bacterial Proteins ,Rickettsiaceae ,Species Specificity ,medicine ,Humans ,Blood culture ,Bacteriological Techniques ,Staining and Labeling ,biology ,medicine.diagnostic_test ,Fatty Acids ,Acridine orange ,bacterial infections and mycoses ,biology.organism_classification ,medicine.disease ,Acridine Orange ,Aerobiosis ,Trench Fever ,Culture Media ,Staining ,chemistry ,bacteria ,Bartonella quintana ,Subculture (biology) ,Research Article - Abstract
Bartonella quintana was isolated from 34 BACTEC nonradiometric aerobic resin blood cultures for 10 adults. Nine patients were initially diagnosed by routine acridine orange staining of routine cultures that had been incubated for 8 days. All subcultures grew on chocolate agar within 3 to 12 days (median, 6 days). The PLUS 26 high-volume aerobic resin medium, combined with acridine orange stain and subculture, is an effective system for detection and isolation of B. quintana from blood.
- Published
- 1994
30. Isolation and characterization by immunofluorescence, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, western blot, restriction fragment length polymorphism-PCR, 16S rRNA gene sequencing, and pulsed-field gel electrophoresis of Rochalimaea quintana from a patient with bacillary angiomatosis
- Author
-
Véronique Roux, R. Viraben, F. Ferrier, Max Maurin, Didier Raoult, and Andreas Stein
- Subjects
Adult ,Male ,Microbiology (medical) ,Blotting, Western ,Molecular Sequence Data ,Fluorescent Antibody Technique ,Bacteremia ,Biology ,Polymerase Chain Reaction ,Restriction fragment ,Microbiology ,Bacterial Proteins ,Western blot ,RNA, Ribosomal, 16S ,Pulsed-field gel electrophoresis ,medicine ,Humans ,Rickettsia ,Polyacrylamide gel electrophoresis ,Gel electrophoresis ,AIDS-Related Opportunistic Infections ,Base Sequence ,medicine.diagnostic_test ,Sodium Dodecyl Sulfate ,Drug Resistance, Microbial ,biology.organism_classification ,Bacillary angiomatosis ,medicine.disease ,Molecular biology ,Trench Fever ,Electrophoresis, Gel, Pulsed-Field ,RNA, Bacterial ,Genes, Bacterial ,Angiomatosis, Bacillary ,biology.protein ,Bartonella quintana ,Electrophoresis, Polyacrylamide Gel ,Restriction fragment length polymorphism ,Polymorphism, Restriction Fragment Length ,Research Article - Abstract
Rochalimaea quintana was isolated from the blood of a French human immunodeficiency virus-infected patient with bacillary angiomatosis. The isolate showed the typical growth characteristics of Rochalimaea species and was inert when typical biochemical testing was used. The purpose of the present work was to characterize and compare this new isolate with reference strains of R. quintana, Rochalimaea vinsonii, and Rochalimaea henselae by using immunofluorescence, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), Western blot (immunoblot), restriction fragment length polymorphism-PCR of the citrate synthase gene, 16S rRNA gene sequencing, and pulsed-field gel electrophoresis. SDS-PAGE, Western blot, restriction fragment length polymorphism-PCR with TaqI enzyme, and 16S rRNA gene sequencing could differentiate the three Rochalimaea species and allowed characterization of the French isolate as R. quintana. However, identification of the Rochalimaea isolate to the species level was more easily obtained by immunofluorescence with specific murine antisera. Pulsed-field gel electrophoresis allowed differentiation of the French R. quintana isolate from R. quintana Fuller and may serve as an epidemiological tool.
- Published
- 1994
31. Production ofBartonellaGenus-Specific Monoclonal Antibodies
- Author
-
Hubert Lepidi, Didier Raoult, Bernard La Scola, and Zhongxing Liang
- Subjects
Microbiology (medical) ,Bartonella ,medicine.drug_class ,Clinical Biochemistry ,Immunology ,medicine.disease_cause ,Monoclonal antibody ,Microbiology ,Mice ,Antigen ,Bartonella quintana ,Phthiraptera ,medicine ,Animals ,Humans ,Immunology and Allergy ,Escherichia coli ,Antigens, Bacterial ,Mice, Inbred BALB C ,Bartonella henselae ,biology ,Antibodies, Monoclonal ,bacterial infections and mycoses ,biology.organism_classification ,Antibodies, Bacterial ,Virology ,biology.protein ,bacteria ,Female ,Rabbits ,Microbial Immunology ,Antibody ,Chlamydia trachomatis - Abstract
Monoclonal antibodies (MAbs) which react with heat-resistant proteins with molecular masses of 32 to 33 kDa of 14 differentBartonellaspecies were produced. These antibodies did not react with antigens of 26 diverse bacterial strains by microimmunofluorescence assay except MAb B3D4, which reacted withChlamydia psittaciandChlamydia trachomatisat low titers. The identification of a commonBartonellaantigenic protein will make it possible to later produce a diagnostic antigen by cloning and expressing it inEscherichia coli. Moreover, these MAbs allow allBartonellaspecies to be identified to the genus level.
- Published
- 2001
32. Detection and Culture of Bartonella quintana, Serratia marcescens , and Acinetobacter spp. from Decontaminated Human Body Lice
- Author
-
Pierre-Edouard Fournier, Didier Raoult, Bernard La Scola, and Philippe Brouqui
- Subjects
DNA, Bacterial ,Microbiology (medical) ,Pediculus humanus ,Polymerase Chain Reaction ,Sensitivity and Specificity ,Microbiology ,Bartonella quintana ,Staphylococcus epidermidis ,parasitic diseases ,medicine ,Animals ,Humans ,skin and connective tissue diseases ,Axenic ,Decontamination ,Serratia marcescens ,Acinetobacter ,Ethanol ,biology ,Pediculus ,Bacteriology ,bacterial infections and mycoses ,biology.organism_classification ,Isolation (microbiology) ,medicine.disease ,Trench Fever ,Trench fever ,Culture Media ,Ill-Housed Persons ,bacteria ,Iodine - Abstract
As part of a survey for trench fever among homeless people in Marseilles, France, we attempted isolation of Bartonella quintana from body lice. A decontamination protocol of immersion in 70% ethanol with 0.2% iodine was devised and was tested with a laboratory colony of body lice. Lice which had been experimentally contaminated with either Escherichia coli, Staphylococcus epidermidis , or Acinetobacter spp. were successfully decontaminated, and this process did not prevent the culture of B. quintana from these lice. One hundred sixty-one lice obtained from homeless patients were studied by the protocol. B. quintana was isolated on axenic medium from 15 of 161 body lice and was detected in 41 of 161 lice by PCR. Acinetobacter spp. and Serratia marcescens were also isolated from body lice. The sensitivities of PCR and culture of B. quintana were 98 and 36%, respectively. These procedures may be useful for epidemiologic studies of trench fever and for the recovery of strains for characterization and comparison.
- Published
- 2001
33. Rochalimaea henselae sp. nov., a cause of septicemia, bacillary angiomatosis, and parenchymal bacillary peliosis
- Author
-
Leonard N. Slater, D J Brenner, Denise A. Pickett, A G Steigerwalt, and David F. Welch
- Subjects
Adult ,DNA, Bacterial ,Microbiology (medical) ,Rickettsiaceae Infections ,Microbiology ,Rickettsiaceae ,Sepsis ,Sequence Homology, Nucleic Acid ,medicine ,Humans ,Peliosis Hepatis ,Afipia felis ,Bartonellosis ,biology ,Fatty Acids ,Cat-scratch disease ,Middle Aged ,medicine.disease ,biology.organism_classification ,Bacillary angiomatosis ,Bacillary peliosis ,Bartonella elizabethae ,Phenotype ,Angiomatosis, Bacillary ,Bartonella quintana ,Bartonella bacilliformis ,Research Article - Abstract
Nine strains of Rochalimaea spp. that were isolated from patients over a period of 4.5 years were characterized for their enzyme activities, cellular fatty acid compositions, and DNA interrelatedness among Rochalimaea spp., Bartonella bacilliformis, and Afipia felis (cat scratch disease bacillus). All except one isolate, which was Rochalimaea quintana, were determined to belong to a newly proposed species, Rochalimaea henselae sp. nov. After recovery from clinical material, colonies required 5 to 15 days of incubation to become apparent. Cells were small, gram-negative, curved bacilli and displayed twitching motility. Enzyme specificities for amino acid and carbohydrate substrates showed that R. henselae could be distinguished from Rochalimaea vinsonii by L-arginyl-L-arginine and L-lysyl-L-alanine peptidases, but not all strains could be distinguished from R. quintana on the basis of peptidases or carbohydrate utilization. R. henselae also closely resembled R. quintana in cellular fatty acid composition, with both consisting mainly of C18:1, C18:0, and C16:0 fatty acids. However, the strains of R. henselae all contained C18:0 in amounts averaging greater than or equal to 22%, in contrast to R. quintana, which contained this cellular fatty acid in amounts averaging 16 and 18%. DNA hybridization confirmed the identification of one clinical isolate as R. quintana and showed a close interrelatedness (92 to 100%) among the other strains. Under optimal conditions for DNA reassociation, R. henselae showed approximately 70% relatedness to R. quintana and approximately 60% relatedness to R. vinsonii. Relatedness with DNA from B. baciliformis was 43%. R. henselae was unrelated to A. felis. R. henselae is the proposed species of a newly recognized member of the family Rickettsiaceae, which is a pathogen that may be encountered in immunocompromised or immunocompetent patients. Prolonged fever with bacteremia or vascular proliferative lesions are clinical manifestations of the agent.
- Published
- 1992
34. In Vitro Activities of Telithromycin (HMR 3647) against Rickettsia rickettsii , Rickettsia conorii , Rickettsia africae , Rickettsia typhi , Rickettsia prowazekii , Coxiella burnetii , Bartonella henselae , Bartonella quintana , Bartonella bacilliformis , and Ehrlichia chaffeensis
- Author
-
Didier Raoult, André Bryskier, Jean-Marc Rolain, and Max Maurin
- Subjects
Pharmacology ,Bartonella ,biology ,biology.organism_classification ,Coxiella burnetii ,Rickettsia africae ,Rickettsia rickettsii ,Virology ,Microbiology ,Infectious Diseases ,Rickettsia typhi ,Ehrlichia chaffeensis ,Bartonella quintana ,Pharmacology (medical) ,Bartonella bacilliformis - Abstract
In vitro activities of telithromycin compared to those of erythromycin against Rickettsia spp., Bartonella spp., Coxiella burnetii , and Ehrlichia chaffeensis were determined. Telithromycin was more active than erythromycin against Rickettsia , Bartonella , and Coxiella burnetii , with MICs of 0.5 μg/ml, 0.003 to 0.015 μg/ml, and 1 μg/ml, respectively, but was inactive against Ehrlichia chaffeensis .
- Published
- 2000
35. Multispacer Typing To Study the Genotypic Distribution of Bartonella henselae Populations
- Author
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Lynn Guptil, Anna Sander, Bruno B Chomel, Wenjun Li, Soichi Maruyama, Didier Raoult, and Pierre-Edouard Fournier
- Subjects
Microbiology (medical) ,DNA, Bacterial ,Asia ,Genotype ,Epidemiology ,Population ,Molecular Sequence Data ,Biology ,Cat Diseases ,Bartonella quintana ,Bartonella Infections ,Sequence Homology, Nucleic Acid ,Animals ,Cluster Analysis ,Typing ,education ,Genotyping ,Phylogeny ,Genetics ,education.field_of_study ,Molecular Epidemiology ,Bartonella henselae ,Molecular epidemiology ,Sequence Analysis, DNA ,biology.organism_classification ,Virology ,United States ,Bacterial Typing Techniques ,Europe ,Cats ,Multilocus sequence typing ,DNA, Intergenic ,human activities ,Bartonella Infection - Abstract
Bartonella henselae, a worldwide fastidious bacterium, has a feline reservoir and is pathogenic for humans. However, the relationship between human and cat isolates ofB. henselae, as well as its population dynamics and geographic heterogeneity, is not fully understood, in part because of the absence of appropriate typing methods. Multilocus sequence typing (MLST), the most discriminatory genotyping method forB. henselae, identified seven genotypes and suggested that human isolates arose from a limited number of cat isolates. Herein, we estimated the discriminatory power of multispacer typing (MST) by studying 126B. henselaecat isolates from various areas of Europe, Asia, and the United States. We identified the nine most variable intergenic spacers conserved by bothB. henselaeandBartonella quintanagenomes. By comparing the sequences obtained from these nine spacers for each studied isolate, we identified 39 MST genotypes. The distribution of isolates into MST genotypes matched their phylogenetic organization into four clusters. MST showed that European and Asian isolates were different, in contrast with American isolates, but failed to identify pandemic strains. Our study demonstrated that MST is a powerful method for genotypingB. henselaeat the strain level and may serve in studying the population dynamics of this bacterium and understanding the relationships between cat and human isolates. Finally, we provide a free-access MST-Rick online software program (http://ifr48.timone.univ-mrs.fr/MST_BHenselae/mst) that investigators may use to compare their own MST sequences to our database.
- Published
- 2006
36. Bartonella (Rochalimaea) quintana infection in a seronegative hemodialyzed patient
- Author
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Philippe Brunet, Didier Raoult, Y. Berland, V. Moal, Michel Drancourt, and B. Dussol
- Subjects
Adult ,DNA, Bacterial ,Microbiology (medical) ,Bartonella ,Pathology ,medicine.medical_specialty ,Molecular Sequence Data ,Mice ,Bartonella quintana ,Bone Marrow ,Renal Dialysis ,medicine ,Animals ,Humans ,Endocarditis ,DNA Primers ,Base Sequence ,biology ,Bartonellosis ,bacterial infections and mycoses ,biology.organism_classification ,medicine.disease ,Bacillary angiomatosis ,Antibodies, Bacterial ,Pancytopenia ,Virology ,Trench Fever ,Trench fever ,Sjogren's Syndrome ,Animals, Domestic ,Bacteremia ,Cats ,bacteria ,Female ,Research Article - Abstract
Bartonella quintana is a reemerging pathogen responsible for trench fever, endocarditis, bacteremia, and bacillary angiomatosis. We previously reported the first case of a patient with B. quintana-induced chronic adenomegaly, and here we present a report on a second patient. A hemodialyzed patient with Sjögren's syndrome presented with mediastinal adenomegalies and secondary pancytopenia. All diagnostic investigations remained negative, except that a Bartonella-like microorganism was isolated from a bone marrow biopsy. The isolate was identified as B. quintana by a specific mouse polyclonal antibody and by determination of a partial gltA (citrate synthase-encoding) gene and 16S rRNA gene sequences. DNA of the pathogen was also detected in the adenomegaly and in the serum of the patient by PCR amplification of the gltA gene. Anti-B. quintana antibodies were never detected in the patient's serum throughout the 12-month follow-up but were detected in the serum of the patient's cat. The patient's outcome was favorable after treatment with gentamicin. Chronic adenomegaly in seronegative patients is a new clinical entity due to B. quintana.
- Published
- 1996
37. Predominant Outer Membrane Antigens of Bartonella henselae
- Author
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Craig E. Greene, Duncan C. Krause, Frank C. Gherardini, and Matthew R. Chenoweth
- Subjects
Immunology ,Blotting, Western ,Cat Diseases ,Microbiology ,Antigen ,Animals ,Electrophoresis, Gel, Two-Dimensional ,Heat-Shock Proteins ,Antigens, Bacterial ,Bartonella henselae ,CATS ,biology ,Cell Membrane ,biology.organism_classification ,Molecular Pathogenesis ,Antibodies, Bacterial ,Infectious Diseases ,Membrane protein ,Humoral immunity ,biology.protein ,Angiomatosis, Bacillary ,Cats ,Bartonella quintana ,Parasitology ,Electrophoresis, Polyacrylamide Gel ,Antibody ,Bacterial outer membrane ,Bacterial Outer Membrane Proteins - Abstract
A hallmark of Bartonella henselae is persistent bacteremia in cats despite the presence of a vigorous host immune response. To understand better the long-term survival of B. henselae in cats, we examined the feline humoral immune response to B. henselae outer membrane (OM) proteins in naturally and experimentally infected cats. Initially, a panel of sera ( n = 42) collected throughout North America from naturally infected cats was used to probe B. henselae total membranes to detect commonly recognized antigens. Twelve antigens reacted with sera from at least 85% of cats, and five were recognized by sera from all cats. To localize these antigens further, OMs were purified on discontinuous sucrose density step gradients. Each membrane fraction (OM, hybrid or inner membrane [IM]) contained less than 1% of the total malate dehydrogenase activity (soluble marker), indicating very little contamination by cytoplasmic proteins. FtsI, an integral IM cell division protein, was used to identify the low-density fraction (ρ = 1.13 g/cm 3 ) as putative IM (Bartonella quintana heme-binding protein A (HbpA), defined the high-density fraction (ρ = 1.20 g/cm 3 ) as putative OM. Additionally, little evidence of cross-contamination between the IM and OM was evident by two-dimensional gel electrophoresis. When purified OMs were probed with feline sera, antigenic proteins profiles were very similar to those observed with total membranes, indicating that many, but not all, of the immunoreactive proteins detected in the initial immunoblots were OM components. Interestingly, two-dimensional immunoblots indicated that B. henselae LPS and members of the Hbp family of proteins did not appear to stimulate an humoral response in any infected cats. Seven proteins were recognized by at least 70% of sera tested, but only three were recognized by all sera. Nanospray-tandem mass spectrometry was used to identify OM components, including the immunodominant OM proteins. Recognition of the nonimmunogenic nature of the major OM components, such as LPS, and identification of the predominant immunogens should elucidate the mechanisms by which B. henselae establishes persistent bacteremic infections within cats. Additionally, the common antigens may serve as potential feline vaccine candidates to eliminate the pathogen from its animal reservoir.
- Published
- 2004
38. Pericardial Effusion in a Homeless Man Due to Bartonella quintana
- Author
-
Pierre-Yves Levy, Pierre-Edouard Fournier, Didier Raoult, and M. Carta
- Subjects
Microbiology (medical) ,Bartonella ,Adult ,Male ,Chlamydiology and Rickettsiology ,Pericardial effusion ,Polymerase Chain Reaction ,Pericardial Effusion ,Microbiology ,Pericarditis ,Bartonella quintana ,hemic and lymphatic diseases ,medicine ,Endocarditis ,Humans ,cardiovascular diseases ,DNA Primers ,biology ,Base Sequence ,Amoxicillin ,biology.organism_classification ,medicine.disease ,Bacillary angiomatosis ,bacterial infections and mycoses ,Virology ,Trench fever ,Trench Fever ,Anti-Bacterial Agents ,Treatment Outcome ,Ill-Housed Persons ,bacteria ,Rickettsia conorii - Abstract
Bartonella quintana may cause trench fever, endocarditis, bacillary angiomatosis, and chronic bacteremia, and a reemergence among homeless populations in cities has been noted. Pericarditis from Rickettsia conorii and Coxiella burnetii infection has been described, but there have been no reports of pericarditis due to Bartonella spp. We report a case of pericardial effusion due to Bartonella quintana in a homeless man, diagnosed on the basis of PCR detection of Bartonella quintana in a pericardial biopsy sample and a fourfold rise in antibody titers. The patient recovered within 2 weeks with antibiotics active against bartonellae.
- Published
- 2003
39. Randomized Open Trial of Gentamicin and Doxycycline for Eradication of Bartonella quintana from Blood in Patients with Chronic Bacteremia
- Author
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Didier Raoult, Cédric Foucault, and Philippe Brouqui
- Subjects
Adult ,Male ,medicine.medical_specialty ,medicine.drug_class ,Antibiotics ,Bacteremia ,Clinical Therapeutics ,Gastroenterology ,law.invention ,Randomized controlled trial ,law ,Bartonella quintana ,Internal medicine ,medicine ,Humans ,Pharmacology (medical) ,Antibacterial agent ,Aged ,Pharmacology ,Doxycycline ,biology ,business.industry ,Aminoglycoside ,Middle Aged ,biology.organism_classification ,medicine.disease ,bacterial infections and mycoses ,Trench Fever ,Surgery ,Anti-Bacterial Agents ,Infectious Diseases ,Treatment Outcome ,Chronic Disease ,Ill-Housed Persons ,Gentamicin ,Drug Therapy, Combination ,Female ,Gentamicins ,business ,medicine.drug - Abstract
Chronic Bartonella quintana bacteremia is known to occur in homeless people exposed to lice. We present here the results of an open randomized trial performed to evaluate the efficacy of doxycycline in combination with gentamicin in the eradication of B. quintana bacteremia. From 1 January 2001 to 1 April 2002, homeless people with blood cultures positive for B. quintana were randomized to receive either no treatment (untreated controls) or a combination of gentamicin (3 mg/kg of body weight/day intravenously for 14 days) and doxycycline (200 mg/day orally for 28 days). Patients were evaluated from the results of blood cultures performed between day 28 (the end of treatment) and day 90 postinclusion. Intention-to-treat analysis of 20 included patients showed eradication of bacteremia in 7 out of 9 treated patients versus 2 out of 11 untreated controls ( P = 0.01). In the per-protocol analysis, eradication was obtained for 7 out of 7 treated patients versus 2 out of 9 untreated controls ( P = 0.003). This study demonstrates the efficiency of the combination of doxycycline and gentamicin in eradicating B. quintana bacteremia.
- Published
- 2003
40. Five-Member Gene Family of Bartonella quintana
- Author
-
Siv G. E. Andersson, Olof Karlberg, Michael F. Minnick, James A. Carroll, Kate N. Sappington, and Laura S. Smitherman
- Subjects
Sequence analysis ,Lipoproteins ,Immunology ,Molecular Sequence Data ,Biology ,Microbiology ,Tandem repeat ,Bacterial Proteins ,Bartonella quintana ,Gene family ,Amino Acid Sequence ,Gene ,Peptide sequence ,Genetics ,Bartonella henselae ,Base Sequence ,Reverse Transcriptase Polymerase Chain Reaction ,Genetic Complementation Test ,Nucleic acid sequence ,Sequence Analysis, DNA ,biology.organism_classification ,Molecular Pathogenesis ,Infectious Diseases ,Genes, Bacterial ,Multigene Family ,Mutation ,Mutagenesis, Site-Directed ,Hemin ,Parasitology ,Carrier Proteins ,Sequence Alignment - Abstract
Bartonella quintana , the agent of trench fever and an etiologic agent of bacillary angiomatosis, has an extraordinarily high hemin requirement for growth compared to other bacterial pathogens. We previously identified the major hemin receptor of the pathogen as a 30-kDa surface protein, termed HbpA. This report describes four additional homologues that share approximately 48% amino acid sequence identity with hbpA . Three of the genes form a paralagous cluster, termed hbpCAB , whereas the other members, hbpD and hbpE , are unlinked. Secondary structure predictions and other evidence suggest that Hbp family members are β-barrels located in the outer membrane and contain eight transmembrane domains plus four extracellular loops. Homologs from a variety of gram-negative pathogens were identified, including Bartonella henselae Pap31, Brucella Omp31, Agrobacterium tumefaciens Omp25, and neisserial opacity proteins (Opa). Family members expressed in vitro-synthesized proteins ranging from ca. 26.5 to 35.1 kDa, with the exception of HbpB, an ∼55.9-kDa protein whose respective gene has been disrupted by a ∼510 GC-rich element containing variable-number tandem repeats. Transcription analysis by quantitative reverse transcriptase-PCR (RT-PCR) indicates that all family members are expressed under normal culture conditions, with hbpD and hbpB transcripts being the most abundant and the rarest, respectively. Mutagenesis of hbpA by allelic exchange produced a strain that exhibited an enhanced hemin-binding phenotype relative to the parental strain, and analysis by quantitative RT-PCR showed elevated transcript levels for the other hbp family members, suggesting that compensatory expression occurs.
- Published
- 2003
41. Value of Microimmunofluorescence for Diagnosis and Follow-up of Bartonella Endocarditis
- Author
-
Jean-Luc Mainardi, Didier Raoult, and Pierre-Edouard Fournier
- Subjects
Microbiology (medical) ,Bartonella ,medicine.medical_specialty ,Clinical Biochemistry ,Immunology ,Population ,Fluorescent Antibody Technique ,Serology ,Bartonella quintana ,Predictive Value of Tests ,Recurrence ,Reference Values ,Internal medicine ,hemic and lymphatic diseases ,Bartonella Infections ,medicine ,Immunology and Allergy ,Endocarditis ,Humans ,Serologic Tests ,education ,education.field_of_study ,Bartonella henselae ,biology ,medicine.disease ,biology.organism_classification ,bacterial infections and mycoses ,Antibodies, Bacterial ,Kinetics ,Infective endocarditis ,bacteria ,Microbial Immunology ,Bartonella Infection ,Follow-Up Studies - Abstract
Bartonella endocarditis is a disease of emerging importance that causes serious complications and high rates of mortality. Due to the fastidious nature of Bartonella species and their high degrees of antibiotic susceptibility, cultures of clinical samples most often remain sterile and valvular biopsy specimens, the best specimens for PCR amplification, are seldom available. Therefore, serology appears to be the easiest diagnostic tool. In order to determine the best cutoff value for serology and its predictive values for the detection of Bartonella endocarditis, we studied 48 patients with culture- and/or PCR-confirmed Bartonella endocarditis. We also applied these serological criteria to 156 patients with blood culture-negative endocarditis. Furthermore, we compared the kinetics of the antibody responses to Bartonella spp. in order to estimate the value of serology for prediction of the occurrence of relapses. A titer of ≥1:800 for immunoglobulin G antibodies to either Bartonella henselae or B. quintana has a positive predictive value of 0.810 for the detection of chronic Bartonella infections in the general population and a value of 0.955 for the detection of Bartonella infections among patients with endocarditis. When this cutoff was applied to 156 patients with blood culture-negative endocarditis, we were able to diagnose Bartonella infections in an additional 45 patients with definite endocarditis for whom a positive Bartonella serology was the only evidence of infection. On follow-up, the kinetics of the decrease in antibody titers were significantly delayed in two patients with relapses. In conclusion, we recommend the determination of antibodies to both B. quintana and B. henselae and the use of a cutoff value of 1:800 for the diagnosis of Bartonella endocarditis. We propose that this criterion, which may also help with the detection of late relapses, be included as a major criterion in the Duke criteria for the diagnosis of infective endocarditis.
- Published
- 2002
42. Natural History of Bartonella Infections (an Exception to Koch’s Postulate)
- Author
-
Véronique Jacomo, Patrick J. Kelly, and Didier Raoult
- Subjects
Microbiology (medical) ,Bartonella ,Clinical Biochemistry ,Immunology ,Normal tissue ,Microbiology ,Species Specificity ,Bartonella quintana ,Bartonella Infections ,medicine ,Immunology and Allergy ,Animals ,Humans ,biology ,Cat-Scratch Disease ,Cat-scratch disease ,Endocarditis, Bacterial ,biology.organism_classification ,medicine.disease ,Natural history ,Normal blood ,Minireview ,Bartonella Infection ,Bacteria - Abstract
“I have, on many occasions, examined normal blood and normal tissues using methods that ensure that such organisms are not overlooked, and I have never, in a single instance, found bacteria. I therefore conclude that bacteria do not occur in the blood or tissues of healthy animals or humans” (R
- Published
- 2002
43. Evaluation of Human Seroreactivity to Bartonella Species in Sweden
- Author
-
Russell L. Regnery, J Darelid, Eva Hjelm, Lars Wesslén, Göran Friman, Lars Blad, Martin Holmberg, Svena McGill, Christian Ehrenborg, and L Engstrand
- Subjects
Microbiology (medical) ,Bartonella ,Adult ,Male ,Adolescent ,Chlamydiology and Rickettsiology ,Population ,Blood Donors ,Serology ,medicine ,Humans ,education ,Aged ,Retrospective Studies ,education.field_of_study ,Antigens, Bacterial ,Bartonella henselae ,biology ,Bartonellosis ,Middle Aged ,biology.organism_classification ,medicine.disease ,bacterial infections and mycoses ,Virology ,Antibodies, Bacterial ,Bartonella elizabethae ,Immunology ,Bartonella quintana ,Female ,Bartonella Infection - Abstract
Among the species that compose the expanding genus Bartonella , thus far only B. henselae and B. quintana have reportedly been isolated from humans in Europe. To evaluate the prevalence of Bartonella infection in Sweden, we conducted a retrospective serological examination of 126 human serum samples. These samples were analyzed for antibodies to B. henselae , B. quintana , and B. elizabethae . Serum samples from 100 blood donors, who spanned the ages of 20 to 60 and had no apparent clinical signs of illness, were also studied as a control group. An immunoglobulin G indirect fluorescence antibody assay revealed 4 and 8.3% Bartonella positivity rates for the blood donor and patient group, respectively, when a cutoff titer of ≥64 was chosen. Among the blood donors, four were seropositive to B. elizabethae ; one of these also had concordant positive titer to B. henselae . In the patient group, 14 serum samples were positive against Bartonella spp. These serum specimens represented nine patients. In three of these seropositive patients, paired serum samples displayed a fourfold increase in antibody titer to at least one of the three antigens. These three patients are discussed. In this report we also present a case study of a 60-year-old Swedish male with fatal myocarditis. Postmortem serological analysis revealed a high titer against B. elizabethae . PCR and nucleotide sequencing of the myocardial tissue from this patient, and of liver tissue from one of the other three patients, showed sequences similar to B. quintana . The age, geographical origin, animal contacts, and serological response pattern to the different Bartonella antigens differed among the four patients. This study substantiates the presence of Bartonella spp. in Sweden, documents the seroreactivity to three Bartonella antigens in Swedish patients, and reports the first two cases of B. quintana -like infections in Sweden.
- Published
- 1999
44. Body Lice as Tools for Diagnosis and Surveillance of Reemerging Diseases
- Author
-
Didier Raoult and Véronique Roux
- Subjects
Microbiology (medical) ,Zimbabwe ,Epidemic typhus ,relapsing fever ,Epidemiology ,Burundi ,Louse ,Polymerase Chain Reaction ,Russia ,Bartonella quintana ,biology.animal ,parasitic diseases ,Peru ,medicine ,Animals ,Humans ,Rickettsia prowazekii ,skin and connective tissue diseases ,Refugees ,biology ,Borrelia ,Pediculus ,Relapsing Fever ,Lice Infestations ,medicine.disease ,biology.organism_classification ,bacterial infections and mycoses ,Virology ,Trench fever ,Trench Fever ,Insect Vectors ,Congo ,Population Surveillance ,Ill-Housed Persons ,bacteria ,France ,Borrelia recurrentis ,Typhus ,Typhus, Epidemic Louse-Borne - Abstract
Body lice are vectors of three bacteria which cause human disease: Rickettsia prowazekii , the agent of epidemic typhus; Bartonella quintana , the agent of trench fever; and Borrelia recurrentis , the agent of relapsing fever. A recrudescence of body lice is being observed as the numbers of individuals living under social conditions which predispose individuals to infestation have increased. Because this phenomenon may lead to the reemergence of infections transmitted by body lice, we aimed to assess the occurrence and prevalence of the three agents described above in more than 600 body lice collected from infested individuals in the African countries of Congo, Zimbabwe, and Burundi, in France, in Russia, and in Peru. The presence of the three bacteria in each louse was determined by specific PCR amplification, and the identities of the organisms detected were confirmed by determination of the nucleotide base sequences of the amplification products. Using this approach, we were able to confirm the presence of R. prowazekii in lice collected from refugees in Burundi, among whom typhus was epidemic, and the presence of B. quintana in lice collected from all locations except the Congo. B. recurrentis was never found. Molecular approaches are convenient tools for the detection and identification of bacterial DNA in body lice and for the epidemiological study of louse-borne bacteria from countries where no medical and biological laboratory facilities are available.
- Published
- 1999
45. Use of the Cell Division Protein FtsZ as a Means of Differentiating among Bartonella Species
- Author
-
Timothy M. Kelly, Barbara R. Baumstark, and Indira Padmalayam
- Subjects
Microbiology (medical) ,Bartonella ,DNA, Bacterial ,Clinical Biochemistry ,Immunology ,Blotting, Western ,Molecular Sequence Data ,Sequence alignment ,macromolecular substances ,physiological processes ,Polymerase Chain Reaction ,Article ,Microbiology ,Open Reading Frames ,Bacterial Proteins ,Species Specificity ,Bartonella quintana ,Immunology and Allergy ,Animals ,Amino Acid Sequence ,ORFS ,Cloning, Molecular ,FtsZ ,Gene ,Bartonella henselae ,biology ,Base Sequence ,Cat-Scratch Disease ,Sequence Analysis, DNA ,biology.organism_classification ,bacterial infections and mycoses ,Trench Fever ,Cytoskeletal Proteins ,biology.protein ,bacteria ,Bartonella bacilliformis ,Rabbits ,biological phenomena, cell phenomena, and immunity ,Sequence Alignment - Abstract
Genes coding for homologs of the highly conserved cell division protein FtsZ were isolated from Bartonella henselae and Bartonella quintana , the causative agents of cat scratch disease and trench fever, respectively. DNA fragments coding for the ftsZ open reading frames (ORFs) were cloned into Escherichia coli following PCR amplification with primers based on the ftsZ sequence of the closely related species Bartonella bacilliformis . The amino acid sequences predicted from the cloned B. henselae and B. quintana ftsZ ORFs are 81 to 83% identical to the corresponding protein in B. bacilliformis . Like the FtsZ protein of B. bacilliformis , the B. henselae and B. quintana homologs are about twice as large as the FtsZ proteins reported in most other organisms. Localized sequence differences within the C-terminal coding regions of the Bartonella ftsZ genes were used as the basis for species-specific identification of these organisms at both the DNA and protein levels. Oligonucleotide primers which permit the amplification of an ftsZ fragment from each of the Bartonella species without amplifying DNA from the other two species were designed. Anti-FtsZ antisera raised in rabbits against synthetic peptides corresponding to the relatively divergent C-terminal regions were shown via Western blot analysis to react only with the FtsZ protein from the cognate Bartonella species. These observations raise the possibility that the differences in ftsZ sequences can be used as the basis for diagnostic tests to differentiate among these closely related pathogens.
- Published
- 1998
46. Complete Genome Sequence of Bartonella quintana, a Bacterium Isolated from Rhesus Macaques
- Author
-
Yigang Tong, Wu-Chun Cao, Hong Yang, Jie-Ying Bai, Yong Huang, Wei Liu, and Hao Li
- Subjects
Whole genome sequencing ,Molecular Sequence Data ,Monkey Diseases ,Biology ,bacterial infections and mycoses ,biology.organism_classification ,Macaca mulatta ,Microbiology ,Virology ,Genome ,Trench Fever ,Genome Announcements ,Broad spectrum ,Bartonella quintana ,bacteria ,Animals ,Molecular Biology ,Pathogen ,Genome, Bacterial ,Bacteria - Abstract
Bartonella quintana is a re-emerging pathogen and the causative agent of a broad spectrum of disease manifestations in humans. The present study reports the complete genome of B. quintana strain RM_11, which was isolated from rhesus macaques.
- Published
- 2012
47. Randomized open trial of gentamicin and doxycycline for eradication of Bartonella quintana from blood in patients with chronic bacteremia.
- Author
-
Foucault C, Raoult D, and Brouqui P
- Subjects
- Adult, Aged, Bacteremia drug therapy, Chronic Disease, Drug Therapy, Combination, Female, Ill-Housed Persons, Humans, Male, Middle Aged, Treatment Outcome, Anti-Bacterial Agents therapeutic use, Bartonella quintana, Doxycycline therapeutic use, Gentamicins therapeutic use, Trench Fever drug therapy
- Abstract
Chronic Bartonella quintana bacteremia is known to occur in homeless people exposed to lice. We present here the results of an open randomized trial performed to evaluate the efficacy of doxycycline in combination with gentamicin in the eradication of B. quintana bacteremia. From 1 January 2001 to 1 April 2002, homeless people with blood cultures positive for B. quintana were randomized to receive either no treatment (untreated controls) or a combination of gentamicin (3 mg/kg of body weight/day intravenously for 14 days) and doxycycline (200 mg/day orally for 28 days). Patients were evaluated from the results of blood cultures performed between day 28 (the end of treatment) and day 90 postinclusion. Intention-to-treat analysis of 20 included patients showed eradication of bacteremia in 7 out of 9 treated patients versus 2 out of 11 untreated controls (P = 0.01). In the per-protocol analysis, eradication was obtained for 7 out of 7 treated patients versus 2 out of 9 untreated controls (P = 0.003). This study demonstrates the efficiency of the combination of doxycycline and gentamicin in eradicating B. quintana bacteremia.
- Published
- 2003
- Full Text
- View/download PDF
48. Natural history of Bartonella infections (an exception to Koch's postulate).
- Author
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Jacomo V, Kelly PJ, and Raoult D
- Subjects
- Animals, Bartonella classification, Bartonella Infections microbiology, Bartonella Infections transmission, Bartonella quintana, Cat-Scratch Disease etiology, Endocarditis, Bacterial etiology, Humans, Species Specificity, Bartonella Infections etiology
- Published
- 2002
- Full Text
- View/download PDF
49. FINE STRUCTURE OF RICKETTSIA QUINTANA CULTIVATED IN VITRO AND IN THE LOUSE.
- Author
-
ITO S and VINSON JW
- Subjects
- Animals, In Vitro Techniques, Bartonella quintana, Cell Membrane, Cell Wall, Cytoplasm, DNA, Electrons, Histocytochemistry, Microscopy, Microscopy, Electron, Phthiraptera, RNA, Research, Ribosomes, Rickettsia
- Abstract
Ito, Susumu (Harvard Medical School, Boston, Mass.), and J. W. Vinson. Fine structure of Rickettsia quintana cultivated in vitro and in the louse. J. Bacteriol. 89:481-495. 1965.-Usually rod-shaped, Rickettsia quintana cells measure about 0.2 to 0.5 mu wide and up to 1.6 mu long. The rickettsiae have both an outer cell wall, about 80 A thick, and a plasma membrane, about 70 A thick, each of which is trilaminar. Occasional vesicular invaginations of the plasma membrane occur. The nuclear material, distributed in irregular zones throughout the cytoplasm, appears as a loose network of fine fibrils when postfixed with uranyl acetate and as thick strands or clumps after routine OsO(4) fixation. The cytoplasm is densely packed with numerous granules, presumably ribosomes, about 150 A in diameter. Histochemical studies revealed the presence of both ribonucleic and deoxyribonucleic acids. During binary fission, a constricting furrow is formed by the cell wall and plasmalemma. No difference in fine structure was observed between R. quintana propagated on cell-free media and in the louse.
- Published
- 1965
- Full Text
- View/download PDF
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