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Start Over Author "Anne Maki" Publisher american society for microbiology
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1. Mycobacterium tuberculosis in Ontario, Canada: Insights from IS 6110 Restriction Fragment Length Polymorphism and Mycobacterial Interspersed Repetitive-Unit-Variable-Number Tandem-Repeat Genotyping

Author

Natalia Kurepina, Jennifer L. Guthrie, Steven J. Drews, Frances B. Jamieson, Pamela Chedore, Anne Maki, Daria Pyskir, David C. Alexander, and Barry N. Kreiswirth

Subjects
DNA, Bacterial, Microbiology (medical), Genotype, Minisatellite Repeat, Minisatellite Repeats, Biology, Mycobacterium tuberculosis, Cluster Analysis, Humans, Tuberculosis, Genotyping, Ontario, Genetics, Molecular Epidemiology, Polymorphism, Genetic, Mycobacteriology and Aerobic Actinomycetes, bacterial infections and mycoses, biology.organism_classification, DNA Fingerprinting, Bacterial Typing Techniques, Variable number tandem repeat, Minisatellite, DNA profiling, DNA Transposable Elements, Restriction fragment length polymorphism, Polymorphism, Restriction Fragment Length
Abstract

A collection of 1,308 clinical Mycobacterium tuberculosis isolates from Ontario, Canada, was genotyped by IS 6110 restriction fragment length polymorphism (RFLP) and mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) analysis. RFLP or >12 MIRU-VNTR loci were necessary for resolution of Indo-Oceanic strains. The low clustering rate and high strain diversity indicate that, in Ontario, most tuberculosis results from reactivation of latent infections.

Published
2009
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