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Your search keyword '"Almeida, D F"' showing total 6 results
Start Over Author "Almeida, D F" Publisher american society for microbiology
6 results on '"Almeida, D F"'

5. Adjuvant activity of a nontoxic mutant of Escherichia coli heat-labile enterotoxin on systemic and mucosal immune responses elicited against a heterologous antigen carried by a live Salmonella enterica serovar Typhimurium vaccine strain.

Author

Guillobel HC, Carinhanha JI, Cárdenas L, Clements JD, de Almeida DF, and Ferreira LC

Subjects
Animals, Antigens, Bacterial administration & dosage, Antigens, Bacterial genetics, Bacterial Proteins administration & dosage, Bacterial Proteins genetics, Bacterial Toxins genetics, Bacterial Vaccines administration & dosage, Bacterial Vaccines genetics, Enterotoxins genetics, Escherichia coli genetics, Lipopolysaccharides administration & dosage, Mice, Mice, Inbred BALB C, Mutation, Salmonella typhimurium genetics, Salmonella typhimurium immunology, Vaccines, Synthetic administration & dosage, Vaccines, Synthetic genetics, Adjuvants, Immunologic pharmacology, Antibodies, Bacterial biosynthesis, Bacterial Toxins pharmacology, Enterotoxins pharmacology, Escherichia coli immunology, Escherichia coli Proteins, Fimbriae Proteins, Immunity, Mucosal
Abstract

Systemic and mucosal antibody responses against both the major subunit of colonization factor antigen I (CFA/I) of enterotoxigenic Escherichia coli (ETEC) and the somatic lipopolysaccharide expressed by recombinant bivalent Salmonella vaccine strains were significantly enhanced by coadministration of a detoxified derivative with preserved adjuvant effects of the ETEC heat-labile toxin, LT((R192G)). The results further support the adjuvant effects of LT((R192G)) and represent a simple alternative to improve responses against passenger antigens expressed by orally delivered Salmonella vaccine strains.

Published
2000
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6. Clonal nature of enterotoxigenic Escherichia coli serotype O6:H16 revealed by randomly amplified polymorphic DNA analysis.

Author

Pacheco AB, Soares KC, de Almeida DF, Viboud GI, Binsztein N, and Ferreira LC

Subjects
Bacterial Typing Techniques, Escherichia coli pathogenicity, Escherichia coli Infections microbiology, Humans, Phylogeny, Serotyping, Virulence, Bacterial Toxins biosynthesis, Enterotoxins biosynthesis, Escherichia coli classification, Escherichia coli genetics, Escherichia coli Proteins, Random Amplified Polymorphic DNA Technique
Abstract

The genetic relatedness among 29 enterotoxigenic Escherichia coli strains of serotype O6:H16 was investigated by randomly amplified polymorphic DNA (RAPD) analysis. The strains were isolated in different parts of the world, displayed CS1-CS3 or CS2-CS3 profiles, and expressed heat-labile toxin (LT) and heat-stable toxin; a single strain expressed only LT. Ten RAPD types were distinguished and showed significant similarity, having on average 82% of the amplified bands in common. These results indicated that, irrespective of the different geographical origin or virulence factors, these strains belonged to a widespread clonal group.

Published
1998
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