1. Primary Swine Respiratory Epithelial Cell Lines for the Efficient Isolation and Propagation of Influenza A Viruses.
- Author
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Meliopoulos, Victoria, Cherry, Sean, Wohlgemuth, Nicholas, Honce, Rebekah, Barnard, Karen, Gauger, Phillip, Davis, Todd, Shult, Peter, Parrish, Colin, and Schultz-Cherry, Stacey
- Subjects
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EPITHELIAL cells , *INFLUENZA viruses , *CELL lines , *SWINE , *SWINE influenza - Abstract
Influenza virus isolation from clinical samples is critical for the identification and characterization of circulating and emerging viruses. Yet efficient isolation can be difficult. In these studies, we isolated primary swine nasal and tracheal respiratory epithelial cells and immortalized swine nasal epithelial cells (siNEC) and tracheal epithelial cells (siTEC) that retained the abilities to form tight junctions and cilia and to differentiate at the air-liquid interface like primary cells. Critically, both human and swine influenza viruses replicated in the immortalized cells, which generally yielded higher-titer viral isolates from human and swine nasal swabs, supported the replication of isolates that failed to grow in Madin-Darby canine kidney (MDCK) cells, and resulted in fewer dominating mutations during viral passaging than MDCK cells. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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