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Your search keyword '"Manoil, Colin"' showing total 2 results
Start Over Author "Manoil, Colin" Topic escherichia coli Publisher american society for microbiology
2 results on '"Manoil, Colin"'

1. Role of a small cytoplasmic domain in the establishment of serine chemoreceptor membrane topology.

Author

Kimbrough, Tyler G. and Manoil, Colin

Subjects
*ESCHERICHIA coli, *CYTOGENETICS
Abstract

Discusses the role of the N-terminal domain in the establishment of serine chemoreceptor membrane topology in Escherichia coli. Separation of N and C termini from a central periplasmic domain; Use of alkaline phosphate gene fusions; Effect of elimination of the positive charge of the domain; Requirements of efficient export of C-terminal domain.

Published
1994
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2. Exploiting a Natural Auxotrophy for Genetic Selection.

Author

Ramage, Elizabeth, Gallagher, Larry, and Manoil, Colin

Subjects
*HISTIDINE, *AUXOTROPHY, *DEHYDROGENASES, *BIOMARKERS, *FRANCISELLA, *BACTERIAL growth, *ESCHERICHIA coli
Abstract

We exploited the natural histidine auxotrophy of FrawciseHa species to develop hisD (encodes histidinol dehydrogenase) as a positive selection marker. A shuttle plasmid (pBR103) carrying Escherichia coli hisD and designed for cloning of PCR fragments replicated in both attenuated and highly virulent Francisella strains. During this work, we formulated a simplified defined growth medium for Francisella novicida. [ABSTRACT FROM AUTHOR]

Published
2012
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