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Lawns represent one of the largest cultivated areas in urban landscapes, and in the Upper Midwest of the United States, lawns are typically composed of a small number of cool-season turfgrass species. There is increased interest in enhancing areas dedicated to lawns using flowering species for conservation purposes-for example, to support pollinators. In this study we used a model flowering forb, Kura clover (Trifolium ambiguum M. Bieb.), because-like many flowering species of conservation interest-it is slow to establish and is sensitive to grass competition. We varied the Kura clover seeding rate into four different turfgrass species treatments: kentucky bluegrass (Poa pratensis L.), hard fescue (Festuca brevipila Tracy), tall fescue [Schedonorus arundinaceum (Schreb.) Darbysh.], and perennial ryegrass (Lolium perenne L.) in two separate trials. Establishment and bloom of Kura clover was significantly greater in trial 1 for kentucky bluegrass and hard fescue than tall fescue and perennial ryegrass. In trial 2, Kura clover established significantly greater in kentucky bluegrass compared with tall fescue and perennial ryegrass, whereas Kura establishment in hard fescue was not significantly different from the other treatments. The seeding rate of Kura clover did not affect establishment in either trial. The results from this study suggest kentucky bluegrass and hard fescue are promising turf companion grasses for future forb/turf interseeding research. [ABSTRACT FROM AUTHOR]

Golf courses in the western United States increasingly are being irrigated with recycled water. Research was conducted on eight golf courses in a semiarid region, including three courses with recycled water irrigation for 10 years, three courses with recycled water irrigation for 18 to 26 years, and two courses with surface water for irrigation for 15 and 18 years. Turf quality of kentucky bluegrass (Poa pratensis) (KBG), the most widely used turfgrass species in the United States, was evaluated on 25 roughs from the aforementioned golf courses. Concurrently, KBG shoot samples and soil samples from these sites were collected. Shoots of KBG were analyzed for mineral concentrations, including sodium (Na), calcium (Ca), magnesium (Mg), potassium (K), chlorine (Cl), boron (B), sulfur (S), phosphorus (P), manganese, iron, zinc, copper, and molybdenum. Electrical conductivity (EC) and sodium absorption ratio (SAR) of soil saturated paste were determined. Recycled water irrigation for 10 and >18 years increased clipping Na by 4.3 and 9.9 times and Cl by 1.5 and 1.3 times, respectively. Compared with surface water irrigation, B concentration in KBG shoots increased by 3.5 times and K concentration reduced by 16% on sites with recycled water irrigation for >18 years. Multiple regression analysis was conducted to identify the relationships between mineral concentration in shoots and turf quality. There was a negative linear relationship between turf quality and Na concentration in the shoots (R² = 0.65). Soil SAR in 0 to 20 cm depth was highly associated with KBG shoot Na, as documented by a logarithmic regression of R² = 0.70. Stepwise regression indicated that Na accumulation in the shoots was the leading plant variable causing the decline of turf quality under recycled water irrigation. Therefore, it is reasonable to believe that water treatment and management practices that can reduce soil SAR and Na concentration in KBG shoots would improve turf quality and plant health. [ABSTRACT FROM AUTHOR]

Turf managers are continually seeking improved grasses, management practices, and products that enhance heat and drought tolerance and reduce supplemental irrigation needs. To this end, products like seaweed extract (SWE) have been extensively studied on short-cut (≤12 mm) golf turf and seedlings of various turfgrass species exposed to stress conditions. Few studies, however, have reported SWE effects on mature, higher cut (≥38 mm) cool-season turfgrass swards. A 3-year field study (2013-15) was conducted in Connecticut to determine the effect of various SWE treatments on the normalized difference vegetative index (NDVI) response of nonirrigated kentucky bluegrass (Poa pratensis L.) and tall fescue (Festuca arundinacea Schreb.) turf managed as a lawn and cut at 76.2 mm. Separate experiments for each species were set out as randomized complete block designs with three replicates. Throughout the growing season in each year, various liquid SWEs were applied at a concentration of 9.55 L·ha-1 weekly or 19.1 L·ha-1 biweekly. A nontreated control was included. The study lacked extreme heat stress conditions during the yearly growing seasons, but periodic moisture deficits below normal were present. Within each year, there were no significant SWE effects on the NDVI of either species. The results suggest that there is no improvement in the NDVI by applying SWEs to mature, higher cut cool-season turfgrass lawns under less than extreme heat-stress conditions, water-stress conditions, or both. Because this study was conducted only at one site without extreme stress, further research of SWE applications to established, higher cut cool-season turfgrass lawns should be conducted across different locations and soils to determine the effects of applying SWE to these stands under extreme heat-stress conditions, water-stress conditions, or both. [ABSTRACT FROM AUTHOR]

Rusts are destructive fungal diseases that can cause severe thinning and unattractive discoloration of kentucky bluegrass (KBG; Poa pratensis L.). Currently, turfgrass breeding programs rely on field evaluations to screen KBG germplasm for rust resistance; methods that are expensive, labor intensive, and require large turf areas. The availability of a greenhouse-based assay to perform prescreening of KBG germplasm for resistance to rust diseases before field trials would allow breeders to remove the poorest performing plants before field evaluations thus enhancing breeding efficiency. In this study, we set out to develop a reliable, low-cost greenhouse inoculation protocol for the two most common rust pathogens of KBG in temperate growing regions: Puccinia coronata and Puccinia graminis, the causal agents of crown and stem rust, respectively. Using a modified inoculation assay and custom-built plexi-glass chambers adapted from protocols used for cereal rust pathogens, urediniospores of crown and stem rust fungi developed on inoculated plants 10 to 14 days postinoculation. Real-time polymerase chain reaction (PCR) assays, disease symptomology, and morphology of urediniospores confirmed the presence and identity of both rust pathogens from inoculated host tissue. The inoculation protocols described here represent an effective method to accelerate screening of KBG germplasm for resistance to crown and stem rust diseases. Infection of KBG plants in the greenhouse will also allow breeders to maintain populations of crown and stem rust fungi throughout the year, providing a reliable and ongoing source of pathogen inoculum for experimentation and screening in the future. [ABSTRACT FROM AUTHOR]

Adequate turfgrass sod strength for harvesting and handling is typically determined by the producer's past experience and subjective appraisal. This study was conducted to determine the relationship between producer subjective sod-strength assessments and quantitative shear-strength measurements with predominantly kentucky bluegrass (Poa pratensis L.) turf. Across three consecutive growing seasons, 93 samples were collected from sod fields in Rhode Island and assessed for sod strength by subjective and quantitative methods. Producer subjective ratings of sod strength were significantly (P < 0.0001) associated with quantitative measurements of peak force required to shear a sod strip. Minimally acceptable strength occurred most frequently when peak shear force was between 55 and 85 kg⋅m-1 width of sod; whereas preferred sod strength occurred most frequently when peak shear force was between 70 and 140 kg⋅m-1 width of sod. Once peak force exceeded 58 and 86 kg⋅m-1, there was a > 50% probability that sod strength would be judged at least adequate and at preferred strength, respectively, up to a peak force of 140 kg⋅m-1. The results suggest that quantitative measurements of shear strength can be related to producer subjective assessments, and provide unbiased benchmark values to guide management decisions for kentucky bluegrass sod production. [ABSTRACT FROM AUTHOR]

Mixtures of turfgrass seed are commonly used to establish lawns, with kentucky bluegrass (KBG) and perennial ryegrass (PRG) comprising two of the more commonly used species. In the humid regions of the Midwest United States, KBG is a desirable species, but slow germination makes it difficult to establish compared with PRG. The objective of our study was to evaluate establishment rate and species composition over 3 years of a turf stand seeded with different ratios of KBG and PRG (wt:wt) maintained as a lawn. Repeat experiments were initiated in 2007 and 2008 and conducted for 3 years in West Lafayette, IN, with seed mixtures of KBG:PRG of 100:0, 90:10, 80:20, 70:30, 50:50, and 0:100 wt:wt of pure live seed. Plots were seeded late August each year and percent turfgrass cover was rated up to 6 weeks after seeding (WAS). To evaluate stand composition after establishment, percent KBG cover was rated annually in August for 3 years using transect counts after selective removal of PRG with the herbicide chlorsulfuron. Likely as a result of greater crabgrass (Digiaria sp.) competition during establishment in 2007, 100% PRG, 50:50, 70:30, or 80:20 KBG:PRG ratio had the highest percentage turf cover at 6 WAS, whereas there was no difference between treatments at 6 WAS in 2008 when crabgrass competition was lower. Regardless of turf cover during establishment, all treatments except 100% PRG shifted to greater than 95% KBG cover by 3 years after establishment. For the region in which our study was conducted, it may be desirable to seed with a higher proportion (greater than 50%) of PRG to speed initial establishment for customer satisfaction, erosion control, and/or to offset years with high weed pressure. Under lawn conditions similar to our study, seeding ratios with high KBG (80:20 or 90:10 KBG:PRG) will likely shift to a stand composition of greater than 95% KBG within 2 years, whereas all other ratios lower in KBG will likely shift similarly within 3 years. Chemical names used: 2-chloro-N-{[(4-methoxy-6-methyl-1,3,5-triazin-2 yl)amino]carbonyl}benzenesulfonamide (chlorsulfuron) [ABSTRACT FROM AUTHOR]

Municipalities often restrict irrigation of urban landscapes, causing plants to experience drought stress. Few data are available regarding drought resistance of non-turfgrass landscape species. This study evaluated the performance of one turfgrass (Poa pratensis L. 'Apollo') and eight herbaceous landscape species (Achillea millifolium L., Ajuga reptans L. 'Bronze Beauty', Liriope muscari Decne., Pachysandra terminalis Siebold and Zucc, Sedum album L., Thymus serpyllum L., Vinca major L., and Vinca minor L.) during a severe drydown and subsequent recovery. This greenhouse study was conducted in the spring/summer and again in the fall of 2010. S. album performed the best, averaging 254 days to decline to a drought rating of 1 (1 to 9 scale, 1 = dead/dormant and 9 = best quality). L. muscari and P. terminalis also performed well, averaging 86 days to a drought rating of 1. V. minor and V. major declined faster than the previous species, averaging 63 days. A. millifolium, A. reptans, P. pratensis, and T. serpyllum declined the fastest to a drought rating of 1 (mean 52 days). Thereafter, the only species to recover after 60 days of resuming irrigation were P. pratensis [46% pot cover (PC)], S. album (38% PC), and V.. major (35% PC) in the spring/summer study; no species recovered during the fall study. Results indicate S. album, L. muscari, and P. terminalis are the most drought-resistant among the species evaluated in landscapes where severe drought may occur. V. minor and V. major are good selections in less severe droughts as is P. pratensis if periods of dormancy are acceptable. [ABSTRACT FROM AUTHOR]

Tall fescue (Festuca arundinacea) and hybrid bluegrass (Poa pratensis L. x Poa arachnifera) can both be successfully grown in the transition zone of the United States. However, each grass has limitations. Tall fescue is susceptible to the fungai pathogen Rhizoctonia solani, whereas slow establishment and susceptibility to weed infestations limit hybrid bluegrass. Previous studies have shown the benefits of combining kentucky bluegrass with tall fescue in seeding mixtures. Research was conducted to evaluate the impact of two seeding combinations of hybrid bluegrass and tall fescue (one combination seeded at a 1.9:1 seed count ratio favoring tall fescue, the other combination seeded at a 1:1.8 seed count ratio favoring hybrid bluegrass) as well as monocultures of the species on turfgrass cover, weed species infestation, brown patch disease severity caused by R. solani, sod strength and species ecology. The seeding combinations had lower weed density during establishment and greater turf cover than the monoculture of hybrid bluegrass. The monoculture of tall fescue was subjected to more brown patch disease than the seeding combinations during and after the first year of establishment. Brown patch infestations likely reduced tall fescue cover and led to a species shift favoring hybrid bluegrass in the seeding combinations based on tiller count and weight data. Seeding combinations of tall fescue and hybrid bluegrass are beneficial from an epidemiological perspective because they reduce disease and weed infestations compared with monocultures of either species. From an agronomic perspective, the seeding combination favoring tall fescue provided the densest turf, whereas the seeding combination favoring hybrid had the greatest sod strength. Chemical name used: clopyralid (3,6 dichloropyridine-2 carboxylic acid) [ABSTRACT FROM AUTHOR]

Kentucky bluegrass (Poa pratensis L.) is an important facultative apomictic temperate perennial grass species used for both forage and cultivated turf. Through apomixis, this species is able to propagate diverse and odd ploidy levels, resulting in many genetically distinct phenotypes. A wide range of diverse cultivars and accessions kentucky bluegrass have been previously characterized based on pedigree, common turf performance, and morphological characteristics to create a kentucky bluegrass cultivar classification system. The objectives of the current study were to assess the amount genetic divergence among kentucky bluegrass cultivars, experimental selections, and plant collections and revise/update the original pedigree, turf performance, and morphological characteristics kentucky bluegrass classification system using recently described kentucky bluegrass microsatellite [simple sequence repeat (SSR)] markers. In this study, 247 kentucky bluegrass cultivars, experimental selections, and collections were genotyped using 25 SSR markers. SSR markers showed a strong correlation between genetic relatedness as assessed by molecular markers and the original kentucky bluegrass classification system and also provided justification for a revision/update of the classification system. Traditional classification types that were supported by the current SSR analysis include BVMG, Compact, Compact-America, Julia, Mid-Atlantic, Midnight, and Shamrock types. Newly proposed classification types included Cynthia, Jefferson/Washington, Limousine, P-105, Sydsport, and three Eurasian types. The majority of cultivars, experimental selections, and collections were uniquely identified with the current set SSR markers. Genetic relationships of individuals as assessed by SSR markers closely matched known pedigrees. The current set of SSR markers can be used to rapidly genotype and assign new cultivars/accessions to kentucky bluegrass classification types and assess genetic relatedness among individuals and should be considered for use a kentucky bluegrass plant variety protection program. [ABSTRACT FROM AUTHOR]

Trinexapac-ethyl (TE) is a popular plant growth regulator in the turfgrass industry that inhibits gibberellic acid (GA) biosynthesis and effectively reduces leaf elongation and subsequent clipping production. This greenhouse sand culture experiment was conducted to determine effects of TE application on kentucky bluegrass (Poa pratensis L.) responses to salinity stress. The five salinity levels (0, 20, 40, 60, and 80 mM NaCl) were applied in nutrient solutions and TE treatments (0, 1, and 1.7 g/100 m²) were applied twice at 4-week intervals. Under non-saline conditions and low level salinity conditions, application of TE at 1 g/100 m² (TE1) increased turf quality (TQ), leaf total non-structural carbohydrates (TNC), and chlorophyll (Chl) content. In high salinity, TE1 alleviated the decline in TQ, antioxidant enzyme activities, leaf TNC, Chl, and K+ content. In addition, treated turf with TE at 1 g/100 m² had lower proline, Na+, and malondialdehyde (MDA) contents. However, the adverse effects of high salinities were more pronounced when turf was treated by TE at 1.7 g/100 m² (TE1.7), suggesting that effects of TE on salt tolerance vary with its dosages and salinity levels. We concluded that moderate inhibition of GA biosynthesis by TE enhances salt tolerance in kentucky bluegrass and suggest that enhancement is the result of the maintenance of antioxidant activities, leading to more root growth and greater levels of TNC and Chl content. Chemical names used: 4-(cyclopropyl-β-hydroxymethylene)-3, 5-dioxocyclohexanecarboxylic acid ethyl ester (trinexapac-ethyl). [ABSTRACT FROM AUTHOR]

Six field trials were conducted in 2009 and 2010 to study postemergence control of annual bluegrass (Poa annua L. var. Hausskn Timm) in kentucky bluegrass (Poa pratensis L.) with mesotrione. Mesotrione was applied at 11 different rate and application intervals to an area of kentucky bluegrass that was naturally infested with annual bluegrass. Mesotrione rates of 56 g·ha-1 applied two or three times per week for a total of 10 applications or 84 g·ha-1 applied two times per week for a total of seven applications provided consistent control of annual bluegrass but required significant application labor and resulted in minor kentucky bluegrass injury. Other treatments that required fewer applications, 110 g·ha-1 applied twice per week for five applications or 186 g-ha-1 per week for three applications, also achieved high levels of control under high air temperatures, but control levels can vary significantly as temperatures fluctuate and seasons change. Mesotrione can successfully control annual bluegrass in kentucky bluegrass when frequent applications at low rates are applied or when environmental conditions are conducive to control. [ABSTRACT FROM AUTHOR]

Germination of five turfgrass species ['Barrister' kentucky bluegrass (Poa pratensis L.), 'Barvado' tall rescue (Festuca arundinacea Schreb.), 'Premier II' perennial ryegrass (Lolium perenne L.), 'Bargusto' bermudagrass (Cynodon dactylon L. Pers.), and 'Sea Spray' seashore paspalum (Paspalum vaginatum O. Swartz)] from coated (ZEBA® cornstarch coating; Absorbent Technologies Inc., Beaverton, OR) and uncoated seeds was evaluated on both filler paper and agar. Final germination percentage (FGP) and germination rate (GR) were determined at salinity levels of 0.6 (tap water, control), 2.2 (saline groundwater from a local shallow aquifer), and 7.0, 12.5, and 22.5 dS·m-1 [sodium chloride and calcium chloride (1:1, w:w) dissolved in tap water]. Final germination percentage for kentucky bluegrass, perennial ryegrass, and tall fescue was greater in agar at all salinity levels but was unaffected by the medium at any of the salinities except for 7 dS·m-1 for bermudagrass and seashore paspalum. Coated seashore paspalum and coated perennial ryegrass seed exhibited greater germination than uncoated seed at four of the five salinity levels. Seed coating had no effect on FGP of bermudagrass at any salinity level and coated kentucky bluegrass seed showed reduced germination at 0.6 and 7.0 dS·m-1. Final germination percentage for seashore paspalum improved from 22% to 54% at 12.5 dS·m-1 and from 8% to 20% at 22.5 dS·m-1 when coated seed was used instead of uncoated seed. Germination rates were unaffected by salinity levels ranging from 0.6 to 12.5 dS·m-1 and were higher on agar (10%/day) than on paper (8%/day). Our study suggests that the choice of medium can influence the outcome of germination tests and that results can also vary depending on the salinity level tested and whether the seed are coated. [ABSTRACT FROM AUTHOR]

Broad concerns over water shortages and drought where irrigated urban landscapes are common in high desert regions have focused attention on drought tolerance of turfgrass species. We investigated the physiological responses of kentucky bluegrass (KBG) and tall fescue (TF) under a prolonged drought under high desert conditions. The experimental design was a split plot with three replicates. Two irrigation treatments as a whole plot--well-watered and no water--were applied to subplots of 'Midnight' KBG and 'Gazelle' TF. Stomatal conductance (gs), canopy temperature, and predawn leaf water potential were measured over two seasons. KBG gs and leaf water potential decreased faster and to a greater extent than TF in response to soil drying, and KBG was in complete dormancy and brown within 5 weeks after cessation of irrigation. By contrast, TF maintained a green canopy throughout the drought periods, in the nowater plots, TF appeared to consume water from the deepest measured soil profiles (80- to 100-cm depth), whereas KBG used most of the water in the 50- to 60-cm depths. When watered for recovery in late summer, KBG plots were mostly green within 3 weeks after rewatering. The surface temperature of the well-watered plots was 6-13 °C cooler than the no-water plots and TF showed 5-7 °C lower temperature than KBG in no-water plots. TF is suitable for deep soil, exploiting a larger volume of water to avoid drought, whereas KBG's rapid drought avoidance would likely perform better in shallow landscape soils under drought. [ABSTRACT FROM AUTHOR]

Prairie junegrass (Koeleria macrantha) is a perennial, cool-season, native grass that has shown potential for use as a turfgrass species in the northern Great Plains; however, limited information is available on its salt tolerance, in this study, salinity tolerance of four junegrass populations from North America (Colorado, Minnesota, Nebraska, and North Dakota) and two improved turf-type cultivars from Europe ('Barleria' and 'Barkoel') was evaluated and compared with kentucky bluegrass (Poa pratensis), perennial ryegrass (Loliumperenne), sheep rescue (Festuca ovina), hard fescue (F. brevipila), and tall fescue (F. arundinacea). Salinity tolerance was determined based on the predicted salinity level causing 50% reduction of final germination rate (PSLF) and daily germination rate (PSLD) as well as electrolyte leakage (EL), tissue dry weight (DW), and visual quality (VQ) of mature plants. All populations of prairie junegrass showed similar salt tolerance with an average of PSLF and PSLD being 7.1 and 5.3 g⋅L-1 NaCl, respectively, comparable to kentucky bluegrass and hard and sheep fescue but lower than tall fescue and perennial ryegrass. Larger variations were observed in VQ in the junegrasses compared with EL and DW, in which 'Barleria' from the European population showed the highest VQ, following two salt-tolerant grasses, tall fescue and sheep fescue. Nebraska population was the least salt-tolerant within the species but still exhibited similar or higher tolerance than kentucky bluegrass and perennial ryegrass cv. Arctic Green. Overall, junegrass was more salt-sensitive during germination but more tolerant to salinity when mature. Salinity tolerance of junegrass may be further improved through turfgrass breeding because salinity tolerance varied in different populations. [ABSTRACT FROM AUTHOR]

Salinity tolerance ofl2 turfgrasses in four groups, creeping bentgrass (Agrostis stolonifera L.), fescues (Festuca spp.), kentucky bluegrass (Poa protests L.), and alkaligrass [Puccinellia distans (Jacq.) Parl.], was evaluated using three germination methods. Seeds were germinated on 1% agar medium, on germination paper, or in a hydroponic system under salinity levels of 0,5,10,15, or20g⋅L-1 NaCl. Germination rate and seedling growth of each grass were determined. Salinity reduced the final germination rate (FGR), daily germination rate (DGR), and seedling leaf area (LA) in all tests. On agar medium, no significant difference in salinity tolerance was observed among the four turf groups; however, 'Turf Blue' kentucky bluegrass with a corn starch-based coating (coated 'Turf Blue') showed a significant higher salinity tolerance than the uncoated one. Using germination paper, creeping bentgrass required the highest salinity level to cause 50% reduction in FGR followed by alkaligrass, fescues, and kentucky bluegrass. Kentucky bluegrass required the lowest salinity level (9.5 g⋅L-1) to reduce DGR by 50%. With the hydroponic system, alkaligrass required a salinity level of 26.3 g⋅L-1 to reduce FGR by 50%, the highest among the four groups. Alkaligrass showed again the highest salinity tolerance with an average of 12.7 g⋅L-1 needed to reduce LA by 50%. Among the grasses, coated 'Turf Blue' kentucky bluegrass, 'Declaration' creeping bentgrass, and 'Fults' alkaligrass showed the highest salinity tolerance when evaluated on agar medium, on germination paper, or in the hydroponic system, respectively. The present study determined the salinity tolerance of 12 turfgrasses at seed germination and early seedling growth stages and showed that the germination method was a factor affecting the evaluation result and it should be considered in a seed germination test of turfgrass for salinity tolerance. [ABSTRACT FROM AUTHOR]

Understanding turfgrass response to silicon (Si) application under salinity conditions is important to find a way to improve turfgrass salt tolerance for turf management. The objective of the study was to investigate effects of increasing amendment concentrations of Na2SiO3 on turf growth and distribution of Na+ and K+ in seedlings of kentucky bluegrass (KBG) (Poa pratensis L.) under salinity stress. This growth chamber experiment was consisted of a control (no salinity, and no Si) and five Si amendment treatments (0, 0.24, 0.48, 0.72, and 0.96 g Si/kg saline soil) under 10 g⋅kg-1 salinity conditions. Seed germination rate was significantly increased after 12 d under 0.48 g⋅kg-1 Si treatment. Plant height and canopy coverage were increased under 0.72 g⋅kg-1 Si treatment after 40 and 44 d of treatment, respectively, and tiller number was increased under 0.96 g⋅kg-1 Si treatment compared with 0 Si under saline conditions. With the supplement of Si at 0.48 to 0.96 g⋅kg-1, the ratio of Na+/K+ in shoots was decreased and individual leaf area was increased compared with 0 Si under saline conditions. The increase in individual leaf area was mainly the result of the increase in the leaf blade length. The concentration of K+ in shoots was significantly increased, whereas the concentrations of Na+ in roots were significantly decreased under all Si amendment treatments. The content of K+ was higher in shoots than in roots, but the ratio of Na+/K+ in roots was higher than in shoots in all Si amendment treatments. The results indicate that under saline conditions, Si induced the transfer of K+ from roots to shoots but inhibited the absorption and transfer of Na+, which may contribute to better turf quality and growth with Si treatment under saline conditions. [ABSTRACT FROM AUTHOR]

Kentucky bluegrass (Poa pratensis L.) is an important facultative apomictic temperate perennial grass species used for both forage and cultivated turf. Through apomixis, this species is able to propagate diverse and odd ploidy levels, resulting in many genetically distinct phenotypes. A wide range of diverse cultivars and accessions of kentucky bluegrass have been previously characterized based on common turf performance or morphological characteristics as well as by random amplified polymorphic DNA (RAPD) markers. Although previous characterization efforts have provided valuable information, the use of both morphological characteristics and RAPD markers for genetic diversity analysis has limitations. In the current report, we developed and characterized 88 novel microsatellite markers for kentucky bluegrass. Polymorphism for each marker was assessed in 265 kentucky bluegrass cultivars, experimental selections, collections, and hybrids. The number of alleles for individual microsatellites ranged from four to 81 with an average of 38.3 alleles per simple sequence repeat. These polymorphic microsatellite markers would be useful tools for investigating genetic diversity, creation of genetic linkage maps, assessment of levels of apomixis in cultivars and experimental varieties, and identification of aberrant progeny in apomictic kentucky bluegrass breeding programs. [ABSTRACT FROM AUTHOR]

Six cultivars or selections of kentucky bluegrass (Poa pratensis L.) were grown outdoors from vegetative clones in a gravelly sand medium from Apr. to Sept. 2005 in Riverside, CA, at soil water salinities ranging from 2 to 22 dS·m-1. Cultivars Baron, Brilliant, Cabernet, Eagleton, Midnight, and the selection A01-856, a 'Texas' x kentucky bluegrass hybrid (P. arachnifera x P. pratensis), were evaluated for salt tolerance based on relative and absolute cumulative biomass production, growth rates, leaf chloride concentration, and hyperspectral ground-based remote sensing (RS) canopy reflectance measurements. Remotely sensed indices were linearly correlated with absolute biomass production. Three variations of a Normalized Difference Vegetation Index (NDVIred, NDVIprotein, and NDVIinfra) decreased with increasing salinity-induced changes in grass canopies. An index based on the red-edge inflection point increased (became less negative) with increasing salinity. A Floating Water Band Index decreased with decreased leaf moisture content related to increasing salinity but did not discriminate between cultivars. Shoot spreading rate and NDVIinfra were both related to shoot chloride concentration differences among the kentucky bluegrass (Poa pratensis L) (KBG) cultivars or selections. In theory, non-destructive RS monitoring of above-ground turf development, including NDVIinfra, coupled with measurement of leaf chloride concentrations could be useful in turf salt tolerance breeding programs. Salt tolerance rankings among the KBG cultivars varied depending on the evaluation methods and selection criteria used. Based on absolute and relative biomass, growth rate, and RS, cultivars Baron, Brilliant, and Eagleton were rated as more salt-tolerant than 'Cabernet', 'Midnight', and AO1-856. [ABSTRACT FROM AUTHOR]

Heat-tolerant bluegrass varieties were developed to resist dormancy and retain pigmentation during heat stress events. The objective of this study was to investigate the influence of grass species, nitrogen (N) fertilization, and seasonality on the accumulation patterns of lutein, β-carotene, and chlorophyll a and b in the leaf tissues of turfgrass. The heat-tolerant bluegrass cultivars Dura Blue and Thermal Blue (Poa pratensis L. x Poa arachnifera Torr.), Apollo kentucky bluegrass (Poa pratensis L.), and Kentucky 31 tall fescue (Festuca arundinacea Schreb.) were compared for the accumulation of plant pigments. Evaluations were conducted over 2 consecutive years (Years 4 and 5 after establishment) during two different seasons (spring and summer) and under varying N fertilization. Fertilizer applications of 5, 14, and 27 g N/m²/year resulted in a significant positive correlation for the accumulation of leaf blade lutein and chlorophyll a and b, but not for β-carotene. The accumulation of the four measured plant pigments among the grasses was significantly different with 'Apollo' having the largest concentration of pigments followed by 'Dura Blue', 'Thermal Blue', and finally 'Kentucky 31'. Specifically, when comparing the cultivars Apollo and Kentucky 31, the pigment levels decreased 27%, 26%, 26%, and 23% for lutein, β-carotene, and chlorophyll a and b, respectively. The interesting observation of the analysis of the grass pigment concentrations was that the least reported heat-tolerant cultivar in our study ('Apollo') had the largest measured pigment concentrations. [ABSTRACT FROM AUTHOR]

The objective of this study was to compare differential nutrient responses heat stress in relation to heat tolerance for warm-season (C4) common bermudagrass [Cynodon dactylon (L.) Pers.] and cool-season (C3) kentucky bluegrass (Poa pratensis L.). Both species were exposed to two temperature regimes in growth chambers: optimal day/night temperature conditions (24/20 °C for kentucky bluegrass and 34/30 °C for bermudagrass) or heat stress (10 °C above the respective optimal temperature for each species). Heat injury in leaves was evaluated and the concentrations of several major macronutrients [nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), and magnesium (Mg)[ in both grass species were measured at 0, 7, 14, 21, and 28 days of treatment. Heat stress reduced leaf photochemical efficiency and cellular membrane stability in both species, but bermudagrass leaves exhibited less damage in these parameters than kentucky bluegrass. Heat stress caused a significant decline in N, P, and K concentration, beginning at 7 days in kentucky bluegrass, but had no significant effects on N, P, and K concentration in bermudagrass during the 28-day treatment period. The concentration of Ca and Mg increased under heat stress in both kentucky bluegrass and bermudagrass, but there were no significant differences between the species under optimal or high-temperature conditions, suggesting they were not involved in heat responses in either species. The differential responses of N, P, and K to heat stress could at least partially account for the differences in heat tolerance between the two species and demonstrate the importance of sufficient N, P, and K in turfgrass adaptation to heat stress. [ABSTRACT FROM AUTHOR]

As competition for water resources in areas of western North America intensify as a result of increasing human populations, the sustainability of turfgrass irrigation with limited water resources is questionable. A potential part of the solution is the use of recycled wastewater for landscape irrigation. However, as a result of high levels of salt, successful irrigation with recycled wastewater will likely need to be coupled with selection for increased salinity tolerance in turfgrass species. Additionally, salinity tolerant turfgrass will allow production on soils with inherently high salt levels. The study described here characterized the relative salinity tolerance of 93 accessions of Poa germplasm from the USDA National Plant Germplasm System (NPGS). Control cultivars of tall fescue [Lolium arundinaceum (Schreb.) S.J. Darbyshire], perennial ryegrass (Lolium perenne L.), and kentucky bluegrass (Poa pratensis L.) were also evaluated for comparison. Kentucky bluegrass accessions exhibited a wide range of LD50 (salinity dosage necessary to kill 50% of plants) values from 811 ECdays (PI 369296 from Russia) to 1922 ECdays (PI 371768 from the United States). Five kentucky bluegrass accessions exhibited salinity tolerance equal to or better than that of the tall fescue (LD50 = 1815 ECdays) and perennial ryegrass (LD50 = 1754 ECdays) checks. Thus, there is sufficient variation within this species to develop bluegrass with substantially higher salinity tolerance. [ABSTRACT FROM AUTHOR]

The apomictic breeding behavior of Kentucky bluegrass (Poa pratensis L.) results in many unique cultivars. A classification system was previously developed to characterize the large number of Kentucky bluegrass cultivars into different types. However, many new cultivars have been released since the last refinement of the classification system. The objectives of this study were to determine differences in morphological and agronomic characteristics among select Kentucky bluegrass cultivars representing the major classification types and to determine broad-sense heritability estimates for important morphological (plant height, panicle length, flag leaf height, and flag leaf length and width) and agronomic (rhizome spread) traits in Kentucky bluegrass. A spaced-plant nursery trial was established in the spring of 2003 at Adelphia, NJ. One hundred seventy-three cultivars and selections were planted in a randomized complete block design with three replications. The morphological and agronomic traits listed were measured on spaced plants. High Density type cultivars (formerly the Aggressive type cultivars) had the most prostrate growth habit with plant heights of 33 and 43 cm in 2004 and 2005, respectively. Mid-Atlantic and Texas x Kentucky bluegrass hybrids had the widest rhizome spread (Mid-Atlantic = 73 and 121 cm; Texas x Kentucky bluegrass hybrids = 72 and 122 cm) in 2004 and 2005, respectively. Broad-sense heritability estimates were high for plant height (H = 0.84), panicle length (H = 0.88), flag leaf height (H = 0.85), and rhizome spread (H = 0.85); moderate for flag leaf length (H = 0.71); and low for flag leaf width (H = 0.11). This study characterizes new cultivars into respective groups and identifies the genetic inheritance of important morphological and agronomic traits in Kentucky bluegrass. [ABSTRACT FROM AUTHOR]

Annual bluegrass (Poa annua L.) is a problematic weed in Kentucky bluegrass (Poa pratensis L.). Bispyribac-sodium herbicide can effectively control established annual bluegrass in other cool-season turfgrasses, but unacceptable injury to Kentucky bluegrass has been reported. However, only a few Kentucky bluegrass cultivars have been evaluated. The objective of this study was to determine the extent of intraspecific variability among Kentucky bluegrass cultivars and selections to sequential applications of bispyribac-sodium herbicide. Field experiments were conducted in 2004 and 2005 in New Jersey to determine the response of 55 Kentucky bluegrass cultivars and selections to bispyribac-sodium. The herbicide was applied at 188 g·ha-1 followed 3 weeks later by a second application of 281 g·ha-1. Kentucky bluegrass injury ranged from 8% to 93% 8 weeks after initial treatment (WAIT). 'Blackstone', 'Serene', and A98-962 were the most tolerant to bispyribac-sodium, exhibiting less than 20% injury 8 WAIT. Conversely, 'Washington', 95AN-10, and 'Avalanche' were the most susceptible with up to 93% injury 8 WAIT. The range in tolerance to bispyribac-sodium within Kentucky bluegrass indicates the potential for the identification and development of cultivars with improved tolerance to bispyribac-sodium herbicide. [ABSTRACT FROM AUTHOR]

Creeping bentgrass (Agrostis stolonifera L.; CBG) is a common weed in home lawns and golf course roughs in many regions of the United States. Currently, no herbicides are registered for selective control of CBG in cool-season grasses. The objective of this field study was to evaluate the ability of mesotrione and triclopyr ester to selectively remove CBG from Kentucky bluegrass (Poa pratensis L.) and tall fescue (Festuca arundinacea Schreb.). Mesotrione (0.14 and 0.21 kg⋅ha-1 a.i.) and triclopyr ester (0.56 and 1.12 kg⋅ha-1 a.i.) were applied on a 2-week interval two, three, or four times in Connecticut and Maryland in 2005, and three or four times in Maryland in 2006. Two applications of mesotrione at 0.21 kg⋅ha-1 a.i. provided marginally acceptable CBG control, but three or four applications at 0.14 or 0.21 kg⋅ha-1 a.i. provided excellent CBG control. Mesotrione elicited little or no injury to Kentucky bluegrass, but generally caused objectionable injury in tall fescue for about 7 to 14 d after each application. Triclopyr applied at 0.56 kg⋅ha-1 a.i. reduced CBG cover, but the level of control generally was unacceptable, regardless of application frequency. Three or four applications of triclopyr (1.12 kg⋅ha-1 a.i.) effectively controlled CBG in Connecticut in 2005 and Maryland in 2006. Triclopyr caused no visual injury to tall fescue, regardless of rate or application frequency. Four triclopyr applications to Kentucky bluegrass, however, were phytotoxic and reduced stand density, especially at the high rate (1.12 kg⋅ha-1 a.i.). Three summer applications of mesotrione (0.14 kg⋅ha-1 a.i.) or triclopyr (1.12 kg⋅ha-1 a.i.) provided the best combination of turfgrass safety and CBG control. Chemical names used: [(3,5,6-trichloro-2-pyridinyl)oxy]acetic acid (triclopyr ester); 2-[4-(methylsulfonyl)-2-nitrobenzoyl]-l,3-cycilohexanedione (mesotrione) [ABSTRACT FROM AUTHOR]

Sports field construction contracts in cool-season areas often stipulate a 9- to 12-month period between seeding and opening for play. Seed mixtures are usually dominated by slow-establishing Kentucky bluegrass (KBG; Poa pratensis L.) and contain lower proportions of perennial ryegrass (PRG; Lolium perenne L.) for quick cover. Our objective was to evaluate the effect of planting time on three KBG : PRG mixes, a 100% PRG blend, and their ability to sustain football-type traffic. Field plots were seeded in late summer, as a dormant planting in late fall, and in the following spring. Plots received simulated football traffic, split for one or four weekly games, from mid-August through mid-November of the year in which spring seeding occurred. The experimental design was a strip-split-plot, randomized block with four replications. The study was repeated a second year. All seeding dates provided acceptable turf quality regardless of seed type by September. However, summer seedings of KBG-based mixtures provided better turf quality than mixtures planted in the spring, whereas dormant-seeded mixtures provided the poorest turf quality. Turf seeded with 100% PRG was less sensitive to seeding date, with summer or spring seedings providing similar quality and dormant seedings superior to KBG-based dormant seedings. Summer seedings also resulted in the least amount of broadleaf weeds the next year with dormant seedings having the most weeds, particularly with plots seeded to 95% KBG. All KBG-based seed mixtures provided turf containing ≈50% KBG or more by September, although the amount of KBG remaining after traffic was significantly greatest in plots seeded to 95% KBG and least in plots seeded with 70% KBG. Pure PRG swards provided acceptable turf quality throughout the traffic period but should be used cautiously as a result of winterkill potential and crown rust disease (Puccinia coronata Corda f. sp. agropyri Erikss.). Different amounts of traffic did not affect turf species proportions. The most consistently desirable results may be obtained with a mixture containing 70% to 80% KBG and 30% to 20% PRG, respectively. Mixtures dominated by KBG should be seeded in late summer for best results. [ABSTRACT FROM AUTHOR]

Overseeding of tall fescue and perennial ryegrass into pre-existing stands of kentucky bluegrass is viewed as a strategy to enhance the quality and durability of turfgrass lawns. In a 3-year study, the authors investigated the winter survival and establishment of tall fescue ('Bonsai 2000'), with or without Neotyphodium coenophialum, and perennial ryegrass ('Palmer III'), with or without N. lolii, in the province of Quebec, Canada (≈lat., 54°N), a region characterized by rigorous winter conditions. Grass species were overseeded in June 2003 at two different rates (90 and 180 kg⋅ha-1), in experimental plots from two bioclimatological conditions: Quebec City and Boucherville. Turfgrass establishment and endophyte infection were evaluated during the following two spring and fall periods. Both tall fescue and perennial ryegrass had the capacity to establish and survive winter conditions, but performed best when snow cover was thick and present throughout the winter. The proportion of overseeded plants in the turfgrass stand rarely reached 30% over the years. Although the proportion of tall fescue and perennial ryegrass plants was much reduced in the spring, some tillers survived and were able, later in the season, to compete with kentucky bluegrass as tall fescue and perennial ryegrass populations returned to initial establishment populations each summer in mixed stands. Overwinter endophyte survival was species specific, with N. iolii being able to survive the cold winter but not N. coenophialum, which had a low percent of infection. For the perennial ryegrass--N, iolii association, competition with kentucky bluegrass is a primary factor limiting the increase over time in the proportion of endophyte-infected plants in a turfgrass mixture. Seeding rates did not influence the establishment of either grass species. [ABSTRACT FROM AUTHOR]

Waterlogging (WL) affects the growth and physiological responses of turfgrass. The objectives of this study were to compare the relative WL tolerance of Kentucky bluegrass (Poa pratensis L.) cultivars and to investigate the physiological responses of shoots and roots to WL. Ten cultivars differing in growth habit were subjected to 30 d of WL. The turf quality (TQ) and soil redox potential (Eh), as well as the chlorophyll concentration (Chl), decreased with increasing periods of WL. Among all cultivars, root dry weight (RDW) decreased 16.7% to 39.9% under 10 d and 30.0% to 60% under 30 d of WL, respectively. Waterlogging increased the root electrolyte leakage (REL) from 0.6% to 53.2% under 10 d and from 29.1% to 98.0% under 30 d of WL for all cultivars, respectively. The best correlations were observed between Eh and TQ (r = 0.74), REL and TQ (r = 0.75), RDW and root water-soluble carbohydrate content (RWSC) (r = 0.74), and root oxidase activity and RWSC (r = 0.63), respectively. 'Moonlight', 'Serene', and 'Champagne' showed better tolerance to short-term WL conditions, whereas 'Kenblue' and 'Eagleton' were the least tolerant cultivars. 'Limousine', 'Unique', 'Awesome', 'Julia', and "Midnight II' ranked in the middle group. Variations in WL tolerance among Kentucky bluegrasses could potentially be used for enhancing turfgrass management. [ABSTRACT FROM AUTHOR]

'Dura Blue' and 'Thermal Blue' hybrid bluegrass have been selected for heat and drought tolerance. These grasses offer alternatives to traditional Kentucky bluegrass and tall fescue in the transition zone. Experiments were conducted in two locations during 2003 and 2004 at the University of Tennessee in Knoxville, Tenn. Nitrogen (N) was applied at 50, 150, or 300 kg·ha-1 N per year to 'Apollo' Kentucky bluegrass, 'Dura Blue', and 'Thermal Blue' hybrid bluegrass, and 'Dynasty' and 'Kentucky 31' tall fescue. The main effects of turfgrass and N were significant for color and quality observations. However, their interactions were not significant; therefore, only the main effects are shown. Acceptable turfgrass color (>6) and quality (>6) was observed for all varieties in May, August, and November. All N regimens showed acceptable turfgrass color and quality. However, 150 kg·ha-1 N per year was required to achieve optimum color and quality. 'Kentucky 31' produced higher clipping dry weights when N was applied at 50 kg·ha-1 per year than the other varieties. Nitrogen applied at 150 and 300 kg·ha-1 per year on 'Kentucky 31' and 'Thermal Blue' produced higher clipping dry weights than the other varieties. 'Dynasty' and 'Kentucky 31' had similar brown patch incidences at each nitrogen level. Increases in brown patch incidence occurred as N levels decreased from 300 (21%) to 50 kg·ha-1 per year (31%) for 'Dynasty' and 'Kentucky 31'. Dollar spot incidence occurred on all bluegrass varieties from 7% to 24%. However, dollar spot decreased with increased N fertility. All turfgrass species tested were acceptable for use in the transition zone. [ABSTRACT FROM AUTHOR]

Recent changes in soil testing methodology, the important role of P fertilization in early establishment and soil coverage, and new restrictions on P applications to turf suggest a need for soil test calibration research on Kentucky bluegrass (Poa pratensis L.), tall fescue (Festuca arundinacea Schreb), and perennial ryegrass (Lolium perenne L.). Greenhouse and field studies were conducted for 42 days to examine the relationship between soil lest P levels and P needs for rapid grass establishment using 23 NJ soils with a Mehlich-3 extractable P ranging from 6 to 1238 mg⋅kg-1. Soil tests (Mehlich-1 Mehlich-3, and Bray-1) for extractable P were performed by inductively coupled plasma atomic emission spectroscopy (ICP). Mehlich-3 extractable P and AI were measured to evaluate the ratio of P to AI as a predictor of need for P fertilizer. Kentucky bluegrass establishment was more sensitive to low soil P availability than tall rescue nr perennial ryegrass. Soft test extractants Mehlich-1, Bray-1, or Mehlich-3 were each effective predictors of need for P fertilization. The ratio of P to AI (Mehlich-3 P/AI %) was a better predictor of tall fescue and perennial ryegrass establishment response to P fertilization than soil test P alone. The Mehlich-1, Bray-1, and Mehlich-3 soil test P critical levels for clipping yield response were in the range of 170 to 280 mg⋅kg-1, depending on the soil test extractant, for tall fescue and perennial ryegrass. The Mehlich-3 P/AI (%) critical level was 42% for tall fescue and 33% for perennial ryegrass. Soil test critical levels, based on estimates from clipping yield data, could not be determined for Kentucky bluegrass using the soils in this study. Soil testing for P has the potential to aid in protection of water quality by helping to identify sites where P fertilization can accelerate grass establishment and thereby prevent soil erosion, and by identifying sites that do not need P fertilization, thereby preventing further P enrichment of soil and runoff. Because different grass species have varying critical P levels for establishment, both soil test P and the species should be incorporated into the decision-making process regarding P fertilization. [ABSTRACT FROM AUTHOR]

With the potential introduction of glyphosate-resistant kentucky bluegrass (GRKB) (Poapratensis L.), postemergence herbicides must be identified for renovation from glyphosate-resistant stands or control escaped GRKB. Field experiments were conducted in Indiana, Maryland, New Jersey, and Virginia from July to September 2004 to investigate efficacy of postemergence herbicides for kentucky bluegrass control. Herbicides tested included clethodim at 0.28 kg⋅ha-1 (a.i.), fluazifop-p at 0.43 kg⋅ha-1 (a.i.), formasulfuron at 0.03 kg⋅ha-1 (a.i.), glufosinate at 1.12 kg⋅ha-1 (a.i.), glyphosate at 1.68 kg⋅ha-1 (a.i.), rimsulfuron at 0.03 kg⋅ha-1 (a.i.), sethoxydim at 0.53 kg⋅ha-1 (a.i.), and trifloxysulfuron at 0.03 kg⋅ha-1 (a.i.). One and two applications of each herbicide were applied to separate plots with the sequential applied 4 weeks after initial treatments (WAIT). Single applications of glyphosate completely controlled kentucky bluegrass 4 WAIT in Maryland, New Jersey, and Virginia. Glufosinate completely controlled kentucky bluegrass with one application in Maryland and New Jersey but single and sequential applications provided only 80% to 88% control in Indiana and Virginia. Foramsulfuron and rimsulfuron required sequential applications for complete kentucky bluegrass control 8 WAIT in New Jersey and Maryland but <82% control was obtained in Indiana and Virginia. Trifloxysulfuron controlled kentucky bluegrass 95% to 100% with single applications in Maryland, New Jersey, and Virginia. Single applications of clethodim, fluazifop, and sethoxydim provided minimal stand reductions but sequential applications controlled kentucky bluegrass 65% to 100%. Results suggest glufosinate and trifloxysulfuron have the greatest potential for controlling GRKB while other herbicides provided erratic control and require sequential applications. [ABSTRACT FROM AUTHOR]

Previous research has demonstrated that bispyribac-sodium can selectively control established annual bluegrass (Poa annua L.) in creeping bentgrass (Agrostis stolonifera L.). Annual bluegrass is also a problematic weed in other cool-season turfgrass species. However, the relative tolerance of other cool-season turfgrass species to bispyribac is not known. Field experiments were conducted at Adelphia, N.J., in 2002 and 2003 to gain understanding of the phytotoxic effects that bispyribac may have on kentucky bluegrass (Poa pratensis L.), perennial ryegrass (Lolium perenne L.), tall fescue (Festuca arundinacea (L.) Schreb.), and chewings fine fescue (Festuca rubra L. subsp, commutata Gaud.). Single applications of bispyribac at 37 to 296 g ·ha-1 were applied to mature stands of each species on 11 June, 2002 and 10 June, 2003. Visual injury was evaluated and clippings were collected 35 and 70 days after treatment (DAT). Visual injury at 35 DAT increased as bispyribac rate increased. Kentucky bluegrass was least tolerant to bispyribac with up to 28% injury when applied at 296 g ·ha-1. Injury on other species did not exceed 20%. Initial injury on perennial ryegrass, tall fescue, and chewings fine fescue was primarily in the form of chiorosis, while kentucky bluegrass exhibited more severe stunting and thinning symptoms. Bispyribac at rates from 74 to 296 g ·ha-1 reduced kentucky bluegrass clipping weights by 19% to 35%, respectively, as compared to the untreated control at 35 DAT in 2002. Initial visual injury on perennial ryegrass, tall fescue, and chewings fine fescue dissipated to ≤5% by 70 DAT. However, recovery of kentucky bluegrass was less complete. These studies suggest that bispyribac-sodium has potential to severely injure kentucky bluegrass. Injury on perennial ryegrass, tall fescue, and chewings fine fescue appears to be less severe and persistent; therefore, bispyribac can be used for weed control in these species. Chemical names used: 2,6-bis[(4,6-dimethoxy-2-pyrimidinyl)oxy]benzoic acid (bispyribac-sodium). [ABSTRACT FROM AUTHOR]

Late fall N fertilization of cool-season turfgrass in northern climates is a common practice. Previous research has been focused in climates where freezing temperatures prevail. Research in more moderate northern climates where turf may not go through winter dormancy is scarce. Four fertilizer N sources and an untreated control were applied in four different months (November, December, January, or February) to perennial ryegrass (Lolium perenne L.) in Puyallup, Wash., and to kentucky bluegrass (Poa pratensis L.) In Pullman, Wash., to compare their effects in moderate (Puyallup) and freezing (Pullman) winter climates. In Pullman, only November applications of ammonium sulfate (AmS) or polymer coated sulfur coated urea (PCSCU) enhanced winter turfgrass quality. In Puyallup, November or December application of AmS, PCSCU, or polymer coated urea (PCU) resulted in enhanced winter quality. Polymer coated urea yielded a delayed initial response and a longer residual effect in the spring. Isobutylidenediurea (IBDU) did not improve winter turf quality in either Pullman or Puyallup. Although there was no quality response following January fertilizer application, there was suppression of red thread [Laetisaria fuciformis (McAlpine) Burds.] symptoms in Puyallup, indicating N uptake. Late fall fertilizer N in eastern Washington should be confined to November, using soluble or more quickly available slow-release nitrogen fertilizers. The application window can be extended to December in western Washington, and more slowly available coated ureas can be effectively used. [ABSTRACT FROM AUTHOR]

Ground ivy is a common broadleaf weed that disrupts turf uniformity and is difficult to control. The objective of this field research was to evaluate cultural and chemical control of ground ivy. Increasing annual nitrogen fertilizer applications from 0 to 196 and 293 kg⋅ha-1 reduced ground ivy cover by 24% and 32%, respectively. At 26 weeks after treatment, 1.1 kg⋅ha-1 isoxaben applied in May limited ground ivy spread by 34% compared to the control. Triclopyr, 2,4-D, or fluroxypyr applied at the highest-labeled rate in October provided superior ground ivy control by the following May. Combining an annual fertility program of 196 kg⋅ha-1 nitrogen and an application of 1.1 kg⋅ha-1 isoxaben with or after an application of 2,4-D, fluroxypyr, or triclopyr in the fall can maximize ground ivy control. Chemical names used: N-[3-(1-ethyl-l-methylpropyl)-5isoxazolyl]-2,6- dimethoxybenzamide (isoxaben); [(3,5,6-trichloro-2-pyridinyl)oxy]acetic acid (triclopyr); (2,4-dichlorophenoxy)acetic acid (2,4-D); [(4-amino-3,5-dichloro-6-fluoropyridyl)oxy]acetic acid (fluroxypyr). [ABSTRACT FROM AUTHOR]

Drought is a major factor limiting plant growth, which has been associated with the accumulation of abscisic acid (ABA) in various species. The objective of the study was to determine the relationship between ABA accumulation and drought tolerance for kentucky bluegrass (Poa pratensis L.) during short-term drought stress. Eight kentucky bluegrass cultivars ('Midnight', 'A82-204', 'RSP', 'Alpine', 'Moonlight', 'Brilliant', 'Washington', and 'Baruzo') were subjected to drought stress in a growth chamber. Water relations, gas exchange rate, and ABA content of leaves were determined at various times during drought stress. Turf quality decreased with drought duration for all eight cultivars. Leaf ABA content increased linearly with drought stress within 11 days of treatment; the rate of the increase was negatively related to the rate of turf quality decline. The rate of ABA accumulation during drought stress was positively correlated with the rates of decrease in turf quality (r² = 0.6346), increase in electrolyte leakage (r² = 0.7128), and decrease in relative water content (r² = 0.5913). There were highly significant negative correlations between ABA content and leaf water potential (r² = 0.9074), stomatal conductance (r² = 0.6088), transpiration rate (r² = 0.6581), net photosynthesis rate (r² = 0.6956), and a positive correlation between ABA content and electrolyte leakage (r² = 0.7287). The results indicate that drought tolerance is negatively related to ABA accumulation during short-term drought stress. ABA accumulation in response to drought stress could be used as a metabolic factor to select for drought tolerance in kentucky bluegrass. [ABSTRACT FROM AUTHOR]