1. Defective removal of ribonucleotides from DNA promotes systemic autoimmunity
- Author
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Sophia Blum, Manuel Martínez-Bueno, Christine Wolf, Timothy J. Vyse, John D. Isaacs, Annette Bley, Marta E. Alarcón-Riquelme, Louise S. Bicknell, Markus M. Nöthen, L. Unger, Stuart H. Ralston, Inga Melchers, Axel Roers, Claudia Krug, Osvaldo Chara, Franziska Schmidt, Andrea Leitch, K. Lüthke, Elisabeth Mangold, Min Ae Lee-Kirsch, Norbert Hubner, Claudia Günther, Alice Lorenzi, Young-Ae Lee, Nicole Berndt, Torsten Witte, Deborah S. Cunninghame Graham, Katy R. Astell, Martin Aringer, Andrew P. Jackson, Barbara Kind, Karsten Conrad, Victoria Tüngler, Doryen Bubeck, M. Gahr, Andreas Dahl, Anja Bauerfeind, Stefanie Kretschmer, Sarah Koss, Elizabeth A. Blackburn, Georgia Ramantani, David L. Morris, Dimitra Alexopoulou, Martin A M Reijns, and Annegret Kuhn
- Subjects
Heterozygote ,DNA Repair ,RNase P ,DNA repair ,DNA damage ,Biología ,Ribonucleotide excision repair ,DNA Mutational Analysis ,Ribonuclease H ,Gene Expression ,purl.org/becyt/ford/3.5 [https] ,genetics [Lupus Erythematosus, Systemic] ,Autoimmunity ,Biology ,metabolism [Pyrimidine Dimers] ,medicine.disease_cause ,genetics [Pyrimidine Dimers] ,chemistry.chemical_compound ,Rnase H2 ,metabolism [Interferon Type I] ,medicine ,Humans ,ddc:610 ,RNase H ,Aicardi-Goutieres Syndrome ,Gene ,Cells, Cultured ,genetics [Ribonuclease H] ,Cell Proliferation ,Syndrome AGS ,Mutation ,ribonuclease HII ,Lupus Erythematosus ,genetics [Autoimmunity] ,genetics [Interferon Type I] ,General Medicine ,Molecular biology ,Aicardi Syndrome ,chemistry ,Pyrimidine Dimers ,Cardiovascular and Metabolic Diseases ,Interferon Type I ,biology.protein ,purl.org/becyt/ford/3 [https] ,DNA - Abstract
Genome integrity is continuously challenged by the DNA damage that arises during normal cell metabolism. Biallelic mutations in the genes encoding the genome surveillance enzyme ribonuclease H2 (RNase H2) cause Aicardi-Goutières syndrome (AGS), a pediatric disorder that shares features with the autoimmune disease systemic lupus erythematosus (SLE). Here we determined that heterozygous parents of AGS patients exhibit an intermediate autoimmune phenotype and demonstrated a genetic association between rare RNASEH2 sequence variants and SLE. Evaluation of patient cells revealed that SLE- and AGS-associated mutations impair RNase H2 function and result in accumulation of ribonucleotides in genomic DNA. The ensuing chronic low level of DNA damage triggered a DNA damage response characterized by constitutive p53 phosphorylation and senescence. Patient fibroblasts exhibited constitutive upregulation of IFN-stimulated genes and an enhanced type I IFN response to the immunostimulatory nucleic acid polyinosinic:polycytidylic acid and UV light irradiation, linking RNase H2 deficiency to potentiation of innate immune signaling. Moreover, UV-induced cyclobutane pyrimidine dimer formation was markedly enhanced in ribonucleotide-containing DNA, providing a mechanism for photosensitivity in RNase H2-associated SLE. Collectively, our findings implicate RNase H2 in the pathogenesis of SLE and suggest a role of DNA damage-associated pathways in the initiation of autoimmunity., La lista completa de autores que integran el documento puede consultarse en el archivo, Instituto de Física de Líquidos y Sistemas Biológicos
- Published
- 2015