1. Uncoupling of calcium channel alpha(1) and beta subunits in developing neurons
- Author
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Peter H. Larsen, Naweed I. Syed, J. David Spafford, August B. Smit, Gerald W. Zamponi, Jan van Minnen, and Molecular and Cellular Neurobiology
- Subjects
Central Nervous System ,DNA, Complementary ,Patch-Clamp Techniques ,Time Factors ,P-type calcium channel ,Blotting, Western ,Molecular Sequence Data ,Lymnaea stagnalis ,Biology ,N-type calcium channel ,Transfection ,Biochemistry ,03 medical and health sciences ,Calcium Channels, N-Type ,0302 clinical medicine ,Animals ,Protein Isoforms ,Amino Acid Sequence ,Growth cone ,Molecular Biology ,Cells, Cultured ,In Situ Hybridization ,Phylogeny ,Lymnaea ,030304 developmental biology ,Neurons ,Neurotransmitter Agents ,0303 health sciences ,Sequence Homology, Amino Acid ,Voltage-dependent calcium channel ,Calcium channel ,T-type calcium channel ,Brain ,Cell Biology ,Oligonucleotides, Antisense ,biology.organism_classification ,Cell biology ,Electrophysiology ,R-type calcium channel ,Microscopy, Fluorescence ,Synapses ,RNA ,Chickens ,030217 neurology & neurosurgery - Abstract
Calcium channel beta subunits are key modulators of calcium channel function and membrane targeting of the pore-forming alpha1 subunit. Here we show that an invertebrate (Lymnaea stagnalis) homolog of P/Q- and N-type calcium channels (LCav2), although colocalized with beta subunits in synapses of mature neurons, is physically uncoupled from the beta subunits in the leading edge of growth cones of outgrowing neurons. Moreover, LCav2 channels that mediate transmitter release in mature synapses also participate in neuronal outgrowth in growth cones. The differential association of beta subunits with synaptic calcium channels and those expressed in emergent neuronal growth suggests that beta subunits may play a role in the transformation of Cav2 calcium channel function in immature neurons and mature synapses.
- Published
- 2004